HCS and HCA Platform Features

Automated imaging versatility and accelerated throughput

The CellInsight family of HCS/HCA platforms offers widefield, confocal, or brightfield automated imaging modes, all within one protocol. Engineered with speed in mind, real-time imaging and analysis can be conducted in parallel for the fastest time-to-data possible. Learn more about our unique features including publication-quality imaging, real-time analysis software, cell-painting research, and database management software below.

Publication-quality imaging

The CellInsight family of high-content screening (HCS)/high-content analysis (HCA) platforms are designed to provide automated imaging versatility and accelerated throughput for the fastest time-to-data possible. Choose from widefield, confocal, or brightfield automated imaging modes, all within one protocol. Our instruments provide up to 12 colors to choose from, from ultraviolet through near-infrared fluorescence and multicolor brightfield imaging to enable flexible imaging paradigms. Finally, our systems are engineered with speed in mind, enabling real-time imaging and analysis conducted in parallel for the fastest time-to-data possible.

Breast cancer tissue cells

CellInsight CX7 LED Pro. Human breast cancer histology slides were stained with H&E and KI67-associated DAB markers (imaged with red, green, and blue transmitted light respectively and a 20X objective lens and the 3-channel Histology bioapplication.

A549 cells

CellInsight CX7 LED. Stains: HCS CellMask Blue (blue), Invitrogen Alexa Fluor 488 Phalloidin (green), and Invitrogen Alexa Fluor 750 goat anti–mouse IgG secondary antibody (pink).

BPAE cells

CellInsight CX5. Stains: DAPI (blue), Alexa Fluor 568 phalloidin (red), and goat anti–mouse IgG (H+L) secondary antibody, Alexa Fluor 488 conjugate (green).

HeLa cells

CellInsight CX5. Stains: Hoechst 33342 (blue) and Alexa Fluor 680 phalloidin (yellow).

Onboard real-time analysis software

The Thermo Scientific HCS Studio Cell Analysis Software expertly balances flexibility and ease of use to enable meaningful data analysis from single cells through populations. Parallel imaging and analysis offers true real-time results, fast. 

 

HCS Studio software allows backtracking of each individual cell or cellular event to minimize artifacts in the data. View the corresponding cell level cutout associated with each data point to ensure quality control of what you are actually measuring. 

 

To accelerate assay development, every parameter can be evaluated for assay performance based on Z-prime statistical analysis. This allows you to select your top-performing assays as you scale your screening campaigns. HCS Studio software also features guided workflows with user-friendly visual feedback to facilitate easy assay protocol setup, image acquisition, analysis, and scanning.

 

The HCS Studio software for CellInsight CX7 LED Pro and CellInsight CX7 LZR Pro also feature the Thermo Scientific EurekaScan Finder software. This software significantly helps reduce your instrument runtime and analysis efforts by automating the detection and subsequent capture of events at higher magnification.

 

EurekaScan Finder software offers two different approaches based on the type of sample and user-stipulated criteria that will inform high-content screening.

Scenario I: when studying tissues

The selected objects in the first pass are bigger than the second pass’s field of view (case A), or multiple objects appear in the second pass’s field of view (case B). In this case, the second pass uses a uniformly spaced lattice pattern to define the second pass’s field locations.

Scenario II: when studying spheroids, rare-events

The selected objects in the first pass are smaller than the second pass’s field of view and are spaced apart such that multiple objects are unlikely to be in the same field of view. In this case, the second pass centers the fields of view around each object.

Cell painting research

Cell painting research

Cell Painting multiparameter phenotyping using the Invitrogen Image-iT™ Cell Painting Kit and the CellInsight CX7 LZR Pro platform.

U2OS cells were either untreated or exposed to various concentrations of niclosamide (0–150 μM), gambogic acid (0–2 μM), staurosporine (0–0.5 μM), or amsacrine (0–100 μM) for 24 hours within a 96-well optic thin-bottom plate. Cells were then labeled with Hoechst 33342 dye (Cat. No. H21486), Invitrogen™ Alexa Fluor™ 488 Concanavalin A (Cat. No. C11252), Invitrogen™ SYTO™ 14 Green Fluorescent Nucleic Acid Stain (Cat. No. S7576), Invitrogen™ Alexa Fluor™ 555 Wheat Germ Agglutinin (Cat. No. W32464), Invitrogen™ Alexa Fluor™ 568 Phalloidin (Cat. No. A12380), and Invitrogen™ MitoTracker™ Deep Red FM dye (Cat. No. M46753). After Cell Painting staining, images were acquired with the CellInsight CX7 LZR Pro platform using the 20x 0.8 NA Olympus X Line objective lens and analyzed using the Cell Painting bioapplication in the HCS Studio 5.0 software.

The CX7 LZR Pro HCS platform is equipped with a laser-based autofocus and a powerful sCMOS camera to boost your experiment's assay window and reduce exposure time, providing amazing acquisition speed. Assay speed is further enhanced with true on‑the-fly phenotyping that collapses analysis and image capture into one parallel stream, allowing you to get your data faster. Additionally, the new Thermo Scientific Image-iT™ Cell Painting Kit has been codeveloped with the CellInsight CX7 LZR Pro platform to enable turnkey multiparameter labeling, imaging, and analysis.

 

All these advanced features—paired with a dedicated, all-new, cell-painting bio-application in Thermo Scientific HCS Studio Software and optional Thermo Scientific Amira Software allow the CellInsight CX7 LZR Pro HCS Platform to simplify your most challenging assays, such as cell painting, morphological tracing in 3D, and immune cell to tumor colocalizations.

Store image and database management software

Thermo Scientific Store™ Image and Database Management Software is a SQL™ or Oracle™ relational database used for storing and managing the images and data automatically transferred from an HCS instrument or imported from another imaging source. It allows for rapid, highly scalable, global access to both data and images. Use the Store Image and Database Management Software after you have collected data and images.

Features

  • Automatic transfer of images and data—centralized data storage allowing data and image access from multiple locations
  • Accessible and linked—data is seamlessly linked to its associated image
  • Visualize—view images and data linked together and easily compare images
  • Export—built in export formats for software such as Spotfire™, Excel™, and GeneData Screener™ programs

Be productive from your first day of use with Store Express Image and Database Management Software

Every Thermo Scientific High Content Platform is configured with a base version of Thermo Scientific Store Express Image and Database Management Software. With 10 GB of storage, Store Express Image and Database Management Software helps ensure the security of your data and allows you to be productive from your first day of use. Although it is not a scalable solution, you always have the option to upgrade to the full solution, Thermo Scientific Store Image and Database Management Software as your requirements change.

  • Store Express Image and Database Software included with all high-content platforms
  • Sufficient for moderate use with active data management program
  • Out-of-the-box use with no IT overhead
  • Fully functional with no capabilities hit

Scalable storage

Store Image and Database Management Software’s optional configuration includes:

  • Scalable for use with a SQL or Oracle database to accommodate screens
  • Installed on servers for capacity and management
  • Expert guidance for IT implementation
  • Preferred for industry partners

Expands for higher-throughput screening capabilities

Store Image and Database Management Software expands to a server-based platform, allowing huge amounts of images and data to be managed. With Store Image and Database Management Software, import, convert and spool images from any platform or client running HCS Studio Cell Analysis Software. Share data and images across your organization for a fully collaborative work environment.

Ordering information

Product

Quantity

Cat. No.

 

Thermo Scientific Store Informatics Software Standard Edition 

1 each

S0100015D

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Thermo Scientific Store Informatics Software Academic Edition 

1 each

S0100015A

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Thermo Scientific Store Informatics Software Enterprise Edition 

1 each

S0100015

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High-content screening applications

For Research Use Only. Not for use in diagnostic procedures.