Verified antibody panels for flow cytometry

Verified antibody panels for flow cytometry

Coined in 2010, the term “optimized multicolor immunofluorescence panel”, or OMIP, refers to a thoroughly tested and validated set of antibodies and reagents that can be used together for the multicolor characterization of a specific cell state or response. Published in the journal Cytometry Part A (Wiley Online Library), these OMIPs are designed for flow cytometry, but OMIPs may potentially be defined for image cytometry, fluorescence microscopy, and other polychromatic fluorescence–based methods.

Learn more about Optimized Multicolor Immunofluorescence Panels (OMIPS) in BioProbes 74

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Articles in Cytometry Part A about OMIPs are listed in the table below.

Locate the OMIP you’re interested in, using the table’s search and sort functions. Clicking on the linked text will take you to the article.

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OMIP IDOMIP nameTarget cell type
OMIP-001Quality and phenotype of Ag-responsive human T cells  Human T cells
OMIP-002Phenotypic analysis of specific human CD8+ T-cells using peptide-MHC class I multimers for any of four epitopes  Human T cells
OMIP-003Phenotypic analysis of human memory B cells  Human B cells
OMIP-004In-depth characterization of human T regulatory cells  Human T cells
OMIP-005Quality and phenotype of antigen-responsive rhesus macaque T cells  Rhesus macaque T cells
OMIP-006Phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry  Human T cells
OMIP-007A phenotypic analysis of human natural killer cells  Human NK cells
OMIP-008Measurement of Th1 and Th2 cytokine polyfunctionality of human T cells  Human T cells
OMIP-009Characterization of antigen-specific human T cells  Human T cells
OMIP-010A new 10-color monoclonal antibody panel for polychromatic immunophenotyping of small hematopoietic cell samples  Human hematopoietic cells
OMIP-011Characterization of circulating endothelial cells (CECs) in peripheral blood  Human endothelial cells
OMIP-012Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice  Human leukocytes in humanized mouse
OMIP-013Differentiation of human T-cells  Human T cells
OMIP-014Validated multifunctional characterization of antigen-specific human T cells by intracellular cytokine staining  Human T cells
OMIP-015Human regulatory and activated T-cells without intracellular staining  Human T cells
OMIP-016Characterization of antigen-responsive macaque and human T-cells  Cynomolgus macaque or Human T Cells
OMIP-017Human CD4+ helper T-cell subsets including follicular helper cells  Human T cells
OMIP-018Chemokine receptor expression on human T helper cells  Human T cells
OMIP-019Quantification of human γδT-cells, iNKT-cells, and hematopoietic precursors Human T cells, NK cells
OMIP-020Phenotypic characterization of human γδ T-cells by multicolor flow cytometry  Human T cells
OMIP-021Simultaneous quantification of human conventional and innate-like T-cell subsets  Human T cells
OMIP-022Comprehensive assessment of antigen-specific human T-cell functionality and memory  Human T cells
OMIP-02310-color, 13 antibody panel for in-depth phenotyping of human peripheral blood leukocytes  Human leukocytes
OMIP-024Pan-leukocyte immunophenotypic characterization of PBMC subsets in human samplesHuman leukocytes
OMIP-025Evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining  Human T cells, NK cells
OMIP-026Phenotypic analysis of B and plasma cells in rhesus macaques  Rhesus macaque B cells, plasma cells
OMIP-027Functional analysis of human natural killer cells  Human NK cells
OMIP-028Activation panel for rhesus macaque NK cell subsets  Rhesus macaque NK cells
OMIP-029Human NK-cell phenotypization  Human NK cells
OMIP-030Characterization of human T cell subsets via surface markers  Human T cells
OMIP-031Immunologic checkpoint expression on murine effector and memory T-cell subsets  Mouse T cells
OMIP-032Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland  Mouse leukocytes
OMIP-033A comprehensive single step staining protocol for human T- and B-cell subsets  Human T cells, B cells
OMIP-034Comprehensive immune phenotyping of human peripheral leukocytes by mass cytometry for monitoring immunomodulatory therapies  Human leukocytes
OMIP-035Functional analysis of natural killer cell subsets in macaques  Rhesus macaque NK cells
OMIP-036Co-inhibitory receptor (immune checkpoint) expression analysis in human T cell subsets  Human T cells
OMIP-03716-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets  Human lymphocytes
OMIP-038Innate immune assessment with a 14 color flow cytometry panelHuman lymphocytes
OMIP-039Detection and analysis of human adaptive NKG2C+ natural killer cellsNK cells, lymphocytes
OMIP-040Optimized gating of human prostate cellular subpopulationsHuman leukocytes, epithelia, and stroma
OMIP-041Optimized multicolor immunofluorescence panel rat microglial staining protocolRat primary microglia cells
OMIP-04221‐color flow cytometry to comprehensively immunophenotype major lymphocyte and myeloid subsets in human peripheral bloodHuman PBMCs
OMIP-043Identification of human antibody secreting cell subsetsHuman Fresh or cryopreserved PBMC, bone marrow, spleen, or tonsil cells
OMIP-04428-color immunophenotyping of the human dendritic cell compartmentHuman dendritic cells
OMIP-045Characterizing human head and neck tumors and cancer cell lines with mass cytometryCancer cell lines
OMIP-046Characterization of invariant T cell subset activation in humansHuman PBMCs
OMIP-047High‐Dimensional phenotypic characterization of B cellsHuman PBMCs
OMIP-048 MCQuantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometryPrimary murine lymphocytes from spleen and lymph nodes
OMIP-049Analysis of Human Myelopoiesis and Myeloid NeoplasmsHuman myeloid cells
OMIP-050A 28‐color/30‐parameter Fluorescence Flow Cytometry Panel to Enumerate and Characterize Cells Expressing a Wide Array of Immune Checkpoint MoleculesHuman PBMCs
OMIP-05128-color flow cytometry panel to characterize B cells and myeloid cellsHuman B cells
OMIP-052An 18‐Color Panel for Measuring Th1, Th2, Th17, and Tfh Responses in Rhesus MacaquesRhesus macaque PBMCs
OMIP-053Identification, Classification, and Isolation of Major FoxP3 Expressing Human CD4+ Treg SubsetsHuman PBMCs
OMIP-054Broad Immune Phenotyping of Innate and Adaptive Leukocytes in the Brain, Spleen, and Bone Marrow of an Orthotopic Murine Glioblastoma Model by Mass CytometryMouse T lymphocytes
OMIP-055Characterization of Human Innate Lymphoid Cells from Neonatal and Peripheral BloodHuman lymphoid cells
OMIP-056Evaluation of Human Conventional T Cells, Donor‐Unrestricted T Cells, and NK Cells Including Memory Phenotype by Intracellular Cytokine StainingCryopreserved PBMCs
OMIP-057Mouse γδ T‐Cell Development Characterized by a 14 Color Flow Cytometry PanelMouse thymocytes
OMIP-05830‐Parameter Flow Cytometry Panel to Characterize iNKT, NK, Unconventional and Conventional T CellsHuman PBMCs
OMIP-059Identification of Mouse Hematopoietic Stem and Progenitor Cells with Simultaneous Detection of CD45.1/2 and Controllable Green Fluorescent Protein Expression by a Single Staining PanelMouse hematopoietic Stem cells
OMIP-06030‐Parameter Flow Cytometry Panel to Assess T Cell Effector Functions and Regulatory T CellsHuman PMBCs
OMIP-06120‐Color Flow Cytometry Panel for High‐Dimensional Characterization of Murine Antigen‐Presenting CellsMouse dendritic cells
OMIP-062A 14‐Color, 16‐Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway SamplesHuman PBMCs
OMIP-06328‐Color Flow Cytometry Panel for Broad Human ImmunophenotypingHuman PBMCs
OMIP-064A 27‐Color Flow Cytometry Panel to Detect and Characterize Human NK Cells and Other Innate Lymphoid Cell Subsets, MAIT Cells, and γδ T CellsHuman PBMCs
OMIP-065Dog Immunophenotyping and T‐Cell Activity Evaluation with a 14‐Color PanelDog PBMCs
OMIP-066Identification of Novel Subpopulations of Human Group 2 Innate Lymphoid Cells in Peripheral BloodHuman PBMCs
OMIP-06728‐Color Flow Cytometry Panel to Evaluate Human T‐Cell Phenotype and FunctionHuman PBMCs
OMIP-068High‐Dimensional Characterization of Global and Antigen‐Specific B Cells in Chronic InfectionHuman PBMCs
OMIP-069Forty-Color Full Spectrum Flow CytometryPanel for Deep Immunophenotyping of Major CellSubsets in Human Peripheral BloodHuman leukocytes
OMIP-070NKp46‐Based 27‐Color Phenotyping to Define Natural Killer Cells Isolated From Human Tumor TissuesHuman tumor cells
OMIP-071A 31‐Parameter Flow Cytometry Panel for In‐Depth Immunophenotyping of Human T‐Cell Subsets Using Surface MarkersHuman T-Cells
OMIP-072A 15‐color panel for immunophenotypic identification, quantification, and characterization of leukemic stem cells in children with acute myeloid leukemiaLeukemic stem cells
OMIP-073Analysis of human thymocyte development with a 14‐color flow cytometry panelHuman Thymocytes
OMIP-074Phenotypic analysis of IgG and IgA subclasses on human B cellsHuman PBMCs
OMIP-075A 22-color panel for the measurement of antigen-specific T-cell responses in human and nonhuman primatesHuman and nonhuman primate PBMCs
OMIP-076High-dimensional immunophenotyping of murine T-cell, B-cell, and antibody secreting cell subsetsMouse T-cells
OMIP-077Definition of all principal human leukocyte populations using a broadly applicable 14-color panelHuman leukocytes
OMIP-078A 31-parameter panel for comprehensive immunophenotyping of multiple immune cells in human peripheral blood mononuclear cellsHuman T-cells
OMIP-079Cell cycle of CD4+ and CD8+ naïve/memory T cell subsets, and of Treg cells from mouse spleenMouse splenocytes
OMIP-08029-Color flow cytometry panel for comprehensive evaluation of NK and T cells reconstitution after hematopoietic stem cells transplantationHuman PBMCs
OMIP-081A new 21-monoclonal antibody 10-color panel for diagnostic polychromatic immunophenotypingHuman bone marrow, peripheral blood