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A distinctive feature of the early stages of apoptosis is the activation of caspase enzymes, which participate in the cleavage of protein substrates and in the subsequent disassembly of the cell. We offer a series of caspase assays that allow the simple detection of active caspases in living cells in real time or in cellular lysates or extracts.
CellEvent Caspase-3/7 Detection Reagent is optimized for apoptosis analysis with a simple no-wash protocol that helps preserve delicate apoptotic cells, it is available in both green and red fluorescent forms. Live cells in microplate wells can be monitored in real time for apoptotic activity by measuring fluorescence intensity.
Microplate assay kits for caspase-3/7 measurement provide a choice of substrates with different wavelength emissions. Kits are available using rhodamine 110 or AMC to report caspase activity. Substrates are also available as bulk reagents or predispensed into microplate wells
CellEvent Caspase-3/7 Red vs CellEvent Caspase-3/7 Green Dose Response Curve with fluorescence microplate reader. A673 cells were seeded at 5,000 cells per well and treated with staurosporine at the indicated concentration for five hours at 37°C. Cells induced for apoptosis were stained with powder stock formulations of CellEvent Red or Green Reagent prepared in PBS and added directly to the cultures for 1 hour at 37°C prior to scanning fluorescence signal on the Varioskan LUX multimode microplate reader.
CellEvent Caspase-3/7 Green Detection Reagent | CellEvent Caspase-3/7 Red Detection Reagent | EnzChek Caspase-3 Assay Kit #1, Z-DEVD-AMC Substrate | EnzChek Caspase-3 Assay Kit #2, Z-DEVD-R110 Substrate | |||
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Reporter | DEVD conjugated to a nucleic acid-binding dye | DEVD conjugated to a nucleic acid-binding dye | Z-DEVD-AMC substrate | Z-DEVD-R110 substrate | ||
Ex/Em (nm) | 502/530 | 590/610 | 342/441 | 496/520 | ||
Live cell | Yes | Yes | No | No | ||
Lysate | No | No | Yes | Yes | ||
Enzyme prep | No | No | Yes | Yes | ||
Components | Enzyme substrate for live and fixed cells | Enzyme substrate for live and fixed cells | Includes reference standard and inhibitor | Includes reference standard and inhibitor | ||
For use with (Equipment) | Fluorescence microscope, High-content instrument, Microplate readers | Fluorescence microscope, High-content instrument, Microplate readers | Microplate readers | Microplate readers | ||
Protocol outline |
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Format | 25 µL (400X in DMSO) | 100 µL (400X in DMSO) | 1 vial (good for 100 96-well assays) | 5 vials (good for 500 96-well assays) | 500 assays using 100 µL reaction volume | 500 assays using 100 µL reaction volume |
Cat. No. | C10723 | C10423 | C10430 | C10431 | E13183 | E13184 |
High-sensitivity fluorescence detection for 6-1,536 samples can be quickly performed on the Varioskan ALF or Varioskan LUX Multimode Microplate Reader using Invitrogen reagents to enable optimal detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.