Mach1 Competent Cells

Mach1 T1^R is the fastest-growing chemically competent strain currently available (Figure 1). Their colonies are clearly visible within eight hours of plating the transformed cells (ampicillin selection only). Mach1 T1^R competent cells are phage resistant owing to the tonA (fhuA) deletion in their genotype and are designated as Mach1 T1^R. Phage resistance properties protect cells from lysis by T1, T5, and φ80 phages.

Figure 1. The rapid growth rate of Mach1 T1^RE. coli compared to standard cloning. Mach1 T1^R, XL10-Gold, TOP10, XL1-Blue, and DH5α E. coli were transformed with pUC19 and grown overnight. Each culture was inoculated 1:50 in 50 mL of LB ampicillin in 500 mL flask. Growth was measured every 30 minutes for 5.5 hours. Mach1 T1^R cells reached a desired optical density (e.g., for mini-prep analysis) significantly faster (in 4 hours) than other tested strains.

• Quick doubling time allows to plate and pick colonies the same day
• Minipreps can be performed after four hours of growth (Figure 2)
• Mach1 T1^R cells are available in the convenient single-use One Shot and high-throughput MultiShot formats

Figure 2. Time saved using Mach1 E. coli versus other routine cloning strains.