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Tools for Studying RNA Structure and Function |
By affecting cellular process mechanisms, RNA is believed to play a central role in transcription initiation, elongation, termination, mRNA splicing, as well as retroviral infection of eukaryotic cells.
RNA molecules, typically comprised of single- and double-stranded regions, assemble in complex, three-dimensional structures. It is these structures that go on to affect RNA’s interactions with other nucleic acids, proteins, and small molecules in the aforementioned cellular processes.
To further understand RNA’s structure and function, we have developed an extensive portfolio of products for RNA synthesis and modification.
Used to study base-pairing, ribonuclease (RNase) cleaves at either single- or double-stranded RNA regions. The below RNase can be used for RNA structure studies, RNA sequencing, protein footprinting, and boundary experiments.
RNA structure and function can be determined by the use of RNA polymerases, which are enzymes that produce different types of RNA. Common RNA polymerases are listed below.
See RNA polymerase promoter sequences
Shop additional RNA polymerases
Both RNA structure and function studies use enzymes, such as ligases and transferases, to distinguish sections of RNA that are important to its natural function.
Phosphorylation, or the addition of a phosphoryl group to a molecule, is a modification that can be done on nucleic acids to signal to downstream processes. Phosphatases have been used in studies of RNA function.
Running the same assays with multiple samples? These transcription kits efficiently isolates total RNA from a variety of samples with high yields.
Visualization of nucleic acid fragments is important for studying RNA structure and function.
RNA function is studied through the use of caps and modified UTPs, which allow RNA to either get tagged for degradation or translation.
Technical bulletin: Methods for Enzymatic Nonisotopic Labeling of RNA by In Vitro Transcription