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AccuPrime DNA Polymerases |
Invitrogen AccuPrime DNA polymerases utilize AccuPrime accessory proteins for improved PCR yield and specificity. AccuPrime technology is used in Taq, Pfx, and GC-rich formulations.
AccuPrime technology
The thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and improving PCR specificity and yield. In addition, AccuPrime enzymes are designed with antibodies that are specific to the DNA polymerase, inhibiting its activity at room temperature and providing an automatic hot start. Figure 1 illustrates the mechanism of AccuPrime technology.
Click image to enlarge
Figure 1. AccuPrime technology mechanism.
Comparison of Platinum and AccuPrime DNA polymerases
Properties of Platinum DNA polymerases and AccuPrime DNA polymerases are compared for their use in high-fidelity PCR, hot-start PCR, and GC-rich PCR.
|
| ||
|---|---|---|---|
| Fidelity (vs. Taq) | >300x | 9x | 26x |
| Hot-start modification | |||
| Universal primer annealing | No | No | |
| Amplification range | 20 kb* | ≤20 kb (with Buffer II) | ≤12 kb |
| Amplicon overhang | Blunt | 3′A and blunt | Blunt |
| GC-rich format | No | No | |
| Inhibitor tolerance | No | No | |
| DNA synthesis rate | 15–30 sec/kb | 60 sec/kb | 60 sec/kb |
| Residual bacterial gDNA | ≤1 copy (per 50 μL rxn) | Not tested | Not tested |
| Buffer system | One optimized buffer (GC enhancer not required) | Two buffers (based on template type) | One buffer |
| Product formats | Stand-alone polymerase: Colorless Order now Master mix: Colorless Order now Green** Order now | Stand-alone polymerase: Colorless Order now | Stand-alone polymerase: Colorless Order now |
*Amplification up to 40 kb is possible depending on complexity of template DNA. Learn more about High Fidelity Platinum SuperFi II DNA polymerase.
**Contains green buffer that includes density reagent and two tracking dyes for direct gel loading.
|
| |
|---|---|---|
| Hot-start modification | ||
| Fidelity (vs. Taq) | 1x | 2x |
| Amplification range | ≤5 kb | ≤5 kb |
| Inhibitor tolerance | No | |
| DNA synthesis rate | 15 sec/kb | 60 sec/kb |
| Amplicon overhang | 3′A | 3′A |
| Universal primer annealing | No | |
| Residual bacterial gDNA | ≤1 copy/enzyme unit | Not tested |
| GC-rich format | No | |
| Multiplexing | Up to 15 | Up to 20 |
| Buffer system | One optimized buffer (GC enhancer supplied in a separate vial) | Two buffers (based on template type) |
| Product formats | Stand-alone polymerase: | Stand-alone polymerase: Colorless Order now |
*Contains green buffer that includes density reagent and two tracking dyes for direct gel loading.
|
|
| |
|---|---|---|---|
| GC-rich amplification | >65% GC | >65% GC | >65% GC |
| Hot-start modification | No | ||
| Fidelity (vs. Taq) | >300x | 1x | 2x |
| Amplification range | 20 kb* | ≤5 kb | ≤5 kb |
| Amplicon overhang | Blunt | 3′A | Blunt |
| Inhibitor tolerance | No | ||
| DNA synthesis rate | 15–30 sec/kb | 15 sec/kb | 60 sec/kb |
| Universal primer annealing | No | ||
| Residual bacterial gDNA | ≤1 copy (per 50 μL rxn) | ≤1 copy (per 50 μL rxn) | Not tested |
| Buffer system | One optimized buffer (GC enhancer not required) | One optimized buffer (GC enhancer supplied in a separate vial) | Two-buffer system (based on GC%) |
| Product formats | Stand-alone polymerase: Colorless Order now Master mix: Colorless Order now Green** Order now | Stand-alone polymerase: Colorless Order now Master mix: Colorless Order now Green** Order now | Stand-alone polymerase: Colorless Order now |
*Amplification up to 40 kb is possible depending on complexity of template DNA.
**Contains green buffer that includes density reagent and two tracking dyes for direct gel loading.
|
| ||
|---|---|---|---|
| Fidelity (vs. Taq) | >300x | 9x | 26x |
| Hot-start modification | |||
| Universal primer annealing | No | No | |
| Amplification range | 20 kb* | ≤20 kb (with Buffer II) | ≤12 kb |
| Amplicon overhang | Blunt | 3′A and blunt | Blunt |
| GC-rich format | No | No | |
| Inhibitor tolerance | No | No | |
| DNA synthesis rate | 15–30 sec/kb | 60 sec/kb | 60 sec/kb |
| Residual bacterial gDNA | ≤1 copy (per 50 μL rxn) | Not tested | Not tested |
| Buffer system | One optimized buffer (GC enhancer not required) | Two buffers (based on template type) | One buffer |
| Product formats | Stand-alone polymerase: Colorless Order now Master mix: Colorless Order now Green** Order now | Stand-alone polymerase: Colorless Order now | Stand-alone polymerase: Colorless Order now |
*Amplification up to 40 kb is possible depending on complexity of template DNA. Learn more about High Fidelity Platinum SuperFi II DNA polymerase.
**Contains green buffer that includes density reagent and two tracking dyes for direct gel loading.
|
| |
|---|---|---|
| Hot-start modification | ||
| Fidelity (vs. Taq) | 1x | 2x |
| Amplification range | ≤5 kb | ≤5 kb |
| Inhibitor tolerance | No | |
| DNA synthesis rate | 15 sec/kb | 60 sec/kb |
| Amplicon overhang | 3′A | 3′A |
| Universal primer annealing | No | |
| Residual bacterial gDNA | ≤1 copy/enzyme unit | Not tested |
| GC-rich format | No | |
| Multiplexing | Up to 15 | Up to 20 |
| Buffer system | One optimized buffer (GC enhancer supplied in a separate vial) | Two buffers (based on template type) |
| Product formats | Stand-alone polymerase: | Stand-alone polymerase: Colorless Order now |
*Contains green buffer that includes density reagent and two tracking dyes for direct gel loading.
|
|
| |
|---|---|---|---|
| GC-rich amplification | >65% GC | >65% GC | >65% GC |
| Hot-start modification | No | ||
| Fidelity (vs. Taq) | >300x | 1x | 2x |
| Amplification range | 20 kb* | ≤5 kb | ≤5 kb |
| Amplicon overhang | Blunt | 3′A | Blunt |
| Inhibitor tolerance | No | ||
| DNA synthesis rate | 15–30 sec/kb | 15 sec/kb | 60 sec/kb |
| Universal primer annealing | No | ||
| Residual bacterial gDNA | ≤1 copy (per 50 μL rxn) | ≤1 copy (per 50 μL rxn) | Not tested |
| Buffer system | One optimized buffer (GC enhancer not required) | One optimized buffer (GC enhancer supplied in a separate vial) | Two-buffer system (based on GC%) |
| Product formats | Stand-alone polymerase: Colorless Order now Master mix: Colorless Order now Green** Order now | Stand-alone polymerase: Colorless Order now Master mix: Colorless Order now Green** Order now | Stand-alone polymerase: Colorless Order now |
*Amplification up to 40 kb is possible depending on complexity of template DNA.
**Contains green buffer that includes density reagent and two tracking dyes for direct gel loading.
AccuPrime DNA polymerases: Selection guide
The table below summarizes AccuPrime DNA polymerases and formats available for PCR.
|
|
|
| |
|---|---|---|---|---|
| DNA polymerase(s) | Blend of Taq DNA polymerase and proofreading GB-D enzyme from Pyrococcus species | Recombinant KOD enzyme from Thermococcus species | DNA polymerase cloned from Pyrolobus fumarius | AccuPrime Taq DNA polymerase |
| Hot-start modification | No | |||
| Enhanced specificity with AccuPrime accessory proteins | ||||
| Fidelity (vs. Taq) | 9x | 26x | 2x | 2x |
| Amplicon overhang | 3′A and blunt | Blunt | Blunt | 3′A |
| Product formats | Stand-alone enzyme with the two-buffer system:
| Stand-alone enzyme | Stand-alone enzyme with the two-buffer system:
| Stand-alone enzyme with the two-buffer system:
|
| Order now | Order now | Order now | Order now |
Resources: AccuPrime DNA polymerases
Product info sheets
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Stylesheet for Classic Wide Template adjustments
For Research Use Only. Not for use in diagnostic procedures.