Gene Expression Analysis using Real-Time PCR

Real-time PCR

• Cancer research
• Immuno-Oncology
• Neurodegenerative and complex disease
• Validate results from other technologies (NGS, Microarray)
• Pathway analysis
• Biomarker signatures
• eQTL analysis
• Mid- to high-throughput studies using the  OpenArray platform

We have developed two chemistries for the sensitive, accurate quantification of mRNA using real-time PCR: Applied Biosystems TaqMan probe–based chemistry, and Applied Biosystems SYBR Green dye–based chemistry.

Comparison of TaqMan and SYBR chemistries ›

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• TaqMan Gene Expression Assay formats
  Available formats in pre-configured, configurable, or custom options based on your research needs, throughput, and number of targets.


• Digital PCR (dPCR)
  Rare target detection and Absolute quantification of nucleic acid standards & next-generation sequencing libraries


• TaqMan PreAmp Master Mix
  Amplify your cDNA before using it for the real-time PCR reaction. It will increase the amount of cDNA available for subsequent reactions without creating bias in the relative amount of cDNA represented in your sample. This method can be used to help enable single-cell studies.
  
  
• One-step RT-PCR reagents
  By combining reverse transcription (RT) and real-time PCR amplification (qPCR) reagents into a single reaction, you are able to reduce the number of hands-on manipulations required as well as the total time to process your samples and get data. It is ideal for situations where many samples are being screened for a few targets, or when automating your experiments with liquid handling systems. One potential drawback is that all of the cDNA is used in the qPCR, so none is left over for later use.
  
  
• Cells-to-CT kits and other integrated sample preparation/real-time PCR products
  These products eliminate the need for nucleic acid purification, creating much simpler experimental protocols and reducing the effort for sample preparation to just a few minutes.