TaqMan® OpenArray® technology enables you to achieve the highest sample throughput for mid-density genotyping. In a single work day, 36 TaqMan® OpenArray® Genotyping Plates can be run on the OpenArray® Real-Time PCR Platform to generate data for >110,000 genotypes.

  • High-Throughput Results with Ease
    The QuantStudio™ 12K Flex Real-Time PCR System and QuantStudio™ 12K Flex OpenArray® AccuFill™ System help enable you to process thousands of samples per day without robotics.
  • Simple Workflow
    Each QuantStudio™ 12K Flex OpenArray® plate, encased in an alloy bottom for easy handling, comes preloaded with the assays of your choice—just add sample and master mix, cycle and image.
  • Best Experimental Flexibility
    Choose any combination of pre-designed and custom TaqMan® SNP Genotyping Assays and the sample/SNP format that best meets your project needs. 
  • Reliable and economical
    Uses gold standard TaqMan® MGB probe chemistry in a nanofluidic design that reduces reagent usage
  • Easy-to-Understand Pricing
    No oligo setup fee or hidden costs.

Experimental workflow: find the products you need for every step

Isolate and purify genomic DNA.

Products for Step 1: Prepare Sample

 

MagMAX™ DNA Multi-Sample Kit
MagMAX™ DNA Multi-Sample Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells.

Select or design TaqMan® probes using the TaqMan® OpenArray® Plate Configurator or Custom TaqMan® Assay Design Tool respectively. QuantStudio™ 12K Flex TaqMan® OpenArray® Genotyping Plates are delivered with assays dried down in the through-holes you specify. Mix DNA samples with TaqMan® OpenArray® Master Mix in TaqMan® OpenArray® 384-well sample plate.

 

TaqMan® OpenArray® Genotyping Plates 
TaqMan Genotyping Assays use two allele-specific MGB probes and two PCR primers to provide highly robust and accurate genotyping calls. OpenArray technology uses nanoliter fluidics and can be configured using and contains 3,072 through-holes and can be configured using 6 different formats.

  • See products below
  Configure a Genotyping Plate
Configure TaqMan® OpenArray® Genotyping Plates using pre-designed assays and/or existing custom assay designs from your workspace

Design Your Own Custom Plate
Configure TaqMan® OpenArray® Genotyping Plates using the TaqMan® Custom Assay Design Tool    

Load sample mixes onto QuantStudio™ 12K Flex TaqMan® OpenArray® Genotyping Plate using the QuantStudio™ 12K Flex OpenArray® AccuFill™ System. The plate is encased with an alloy bottom for easy handling. Simply apply the adhesive lid and fill with immersion fluid. You can then perform on-line cycling on the QuantStudio 12K Flex system. To increase your throughput even more, as an option, you can amplify your on the Dual 96-well GeneAmp PCR System 9700 and perform an endpoint plate read using the QuantStudio 12K Flex system; TaqMan® SNP Genotyping Assay MGB probes provide a fluorescence signal for the amplification of each allele.

Recommended Products

 

 QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block
High-throughput results with ease—the powerful QuantStudio™ 12K Flex system with OpenArray® block and AccuFill™ System combination enables the processing of thousands of samples per day without robotics.
  • This product is not orderable online.
    For more information, please contact your local sales representative.
   
 Dual Flat Block GeneAmp® PCR System 9700 (Optional)
GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The System consists of a base module and one of many interchangeable sample block modules. The Dual Flat Block GeneAmp® PCR System 9700 is designed for use with QuantStudio™ 12K Flex Real-Time PCR System with OpenArray block as an option to increase throughput.
   

The data is plotted in an allelic discrimination plot with FAM fluorescence signal on the y-axis and VIC fluorescence signal on the x-axis. Software tools enable converting the raw data into genotype calls (e.g., homozygotes and heterozygotes.)

Software

 

TaqMan® Genotyper Software
TaqMan® Genotyper Software is for use with TaqMan® SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96- & 384-well microtiter plates, and OpenArray® Genotyping Plates and offers a state-of-the-art genotype calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.

   
Step 1 : Prepare Sample

Isolate and purify genomic DNA.

Products for Step 1: Prepare Sample

 

MagMAX™ DNA Multi-Sample Kit
MagMAX™ DNA Multi-Sample Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells.

Step 2 : Select Assays & Configure Plates

Select or design TaqMan® probes using the TaqMan® OpenArray® Plate Configurator or Custom TaqMan® Assay Design Tool respectively. QuantStudio™ 12K Flex TaqMan® OpenArray® Genotyping Plates are delivered with assays dried down in the through-holes you specify. Mix DNA samples with TaqMan® OpenArray® Master Mix in TaqMan® OpenArray® 384-well sample plate.

 

TaqMan® OpenArray® Genotyping Plates 
TaqMan Genotyping Assays use two allele-specific MGB probes and two PCR primers to provide highly robust and accurate genotyping calls. OpenArray technology uses nanoliter fluidics and can be configured using and contains 3,072 through-holes and can be configured using 6 different formats.

  • See products below
  Configure a Genotyping Plate
Configure TaqMan® OpenArray® Genotyping Plates using pre-designed assays and/or existing custom assay designs from your workspace

Design Your Own Custom Plate
Configure TaqMan® OpenArray® Genotyping Plates using the TaqMan® Custom Assay Design Tool    
Step 3 : Amplify DNA & Read

Load sample mixes onto QuantStudio™ 12K Flex TaqMan® OpenArray® Genotyping Plate using the QuantStudio™ 12K Flex OpenArray® AccuFill™ System. The plate is encased with an alloy bottom for easy handling. Simply apply the adhesive lid and fill with immersion fluid. You can then perform on-line cycling on the QuantStudio 12K Flex system. To increase your throughput even more, as an option, you can amplify your on the Dual 96-well GeneAmp PCR System 9700 and perform an endpoint plate read using the QuantStudio 12K Flex system; TaqMan® SNP Genotyping Assay MGB probes provide a fluorescence signal for the amplification of each allele.

Recommended Products

 

 QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block
High-throughput results with ease—the powerful QuantStudio™ 12K Flex system with OpenArray® block and AccuFill™ System combination enables the processing of thousands of samples per day without robotics.
  • This product is not orderable online.
    For more information, please contact your local sales representative.
   
 Dual Flat Block GeneAmp® PCR System 9700 (Optional)
GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The System consists of a base module and one of many interchangeable sample block modules. The Dual Flat Block GeneAmp® PCR System 9700 is designed for use with QuantStudio™ 12K Flex Real-Time PCR System with OpenArray block as an option to increase throughput.
   
Step 4 : Analyze Data

The data is plotted in an allelic discrimination plot with FAM fluorescence signal on the y-axis and VIC fluorescence signal on the x-axis. Software tools enable converting the raw data into genotype calls (e.g., homozygotes and heterozygotes.)

Software

 

TaqMan® Genotyper Software
TaqMan® Genotyper Software is for use with TaqMan® SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96- & 384-well microtiter plates, and OpenArray® Genotyping Plates and offers a state-of-the-art genotype calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.

   

Publications

Access to OpenArray™ publications search

Resources
Support

For Research Use Only. Not for use in diagnostic procedures.