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OpenArray™ plate technology on the QuantStudio™ 12K Flex Real-Time System helps streamline microRNA (miRNA) profiling, at a scale previously unattainable. Achieve the highest sample throughput for mid-density miRNA profiling—in a single work day, one user can process 48 samples with minimal pipetting steps and without the use of robotics.
Be one of the first to use our TaqMan Advanced miRNA chemistry on a TaqMan Array Card or OpenArray Plate. Enable highly sensitive and specific quantification of miRNAs in a high throughput format.
Search for pre-designed 96-well array plates ›
Search for pre-designed 384-well array cards ›
Life Technologies offers a variety of kits for the isolation and analysis of miRNA and other small RNAs, enabling quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).
mirVana™ miRNA Isolation Kit |
Designed to streamline miRNA profiling, Megaplex™ RT Primers are pools of target-specific, stem-loop reverse transcription (RT) primers from TaqMan® MicroRNA Assays. These RT-primer pools are ideal for global miRNA expression profiling; just two pools provide comprehensive coverage of the Sanger miRBase v14 and v15 database content for human and rodent, respectively. The content of each pool matches corresponding QuantStudio™ 12K Flex TaqMan® OpenArray® Human or Rodent MicroRNA Panels. The TaqMan® MicroRNA RT Kit provides optimal TaqMan® MicroRNA Assay performance. This kit is used to convert miRNA to cDNA prior to real-time PCR quantitation.
Recommended Products
Megaplex™ RT Primers | ||
TaqMan® MicroRNA Reverse Transcription Kit |
Megaplex™ PreAmp Primers help you stretch as little as 1 ng of input total RNA into enough cDNA to generate a comprehensive miRNA expression profile. With content matched to specific Megaplex™ RT Primers and QuantStudio™ 12K Flex TaqMan® OpenArray® Human MicroRNA Panels, cDNA prepared by reverse transcription can be preamplified using Megaplex™ PreAmp Primers and TaqMan® PreAmp Master Mix to amplify all miRNAs present in the original sample, significantly increasing the ability to detect low abundance transcripts.
Megaplex™ PreAmp Primers | ||
TaqMan® PreAmp Master Mix |
Load sample mixes onto QuantStudio™ 12K Flex TaqMan® OpenArray® MicroRNA Panels using the QuantStudio™ 12K Flex OpenArray® AccuFill™ System. The plate bottom is encased with an alloy for easy handling. Simply fill with immersion fluid and apply the adhesive lid.
TaqMan® OpenArray® MicroRNA Panels | ||
TaqMan® OpenArray® Real-Time PCR Master Mix
OpenArray® AccuFill™ System The OpenArray® AccuFill™ System loads the sample mix from a 384‐well plate onto the TaqMan® OpenArray® Real-Time PCR plate with minimal human intervention. OpenArray® Sample Tracker Software integrates the sample information from a 384‐well plate file with the assay information contained in the plate setup file. |
Perform a real-time plate read using the QuantStudio™ 12K Flex Real-Time PCR System.
QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block |
Life Technologies offers a variety of kits for the isolation and analysis of miRNA and other small RNAs, enabling quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).
mirVana™ miRNA Isolation Kit |
Designed to streamline miRNA profiling, Megaplex™ RT Primers are pools of target-specific, stem-loop reverse transcription (RT) primers from TaqMan® MicroRNA Assays. These RT-primer pools are ideal for global miRNA expression profiling; just two pools provide comprehensive coverage of the Sanger miRBase v14 and v15 database content for human and rodent, respectively. The content of each pool matches corresponding QuantStudio™ 12K Flex TaqMan® OpenArray® Human or Rodent MicroRNA Panels. The TaqMan® MicroRNA RT Kit provides optimal TaqMan® MicroRNA Assay performance. This kit is used to convert miRNA to cDNA prior to real-time PCR quantitation.
Recommended Products
Megaplex™ RT Primers | ||
TaqMan® MicroRNA Reverse Transcription Kit |
Megaplex™ PreAmp Primers help you stretch as little as 1 ng of input total RNA into enough cDNA to generate a comprehensive miRNA expression profile. With content matched to specific Megaplex™ RT Primers and QuantStudio™ 12K Flex TaqMan® OpenArray® Human MicroRNA Panels, cDNA prepared by reverse transcription can be preamplified using Megaplex™ PreAmp Primers and TaqMan® PreAmp Master Mix to amplify all miRNAs present in the original sample, significantly increasing the ability to detect low abundance transcripts.
Megaplex™ PreAmp Primers | ||
TaqMan® PreAmp Master Mix |
Load sample mixes onto QuantStudio™ 12K Flex TaqMan® OpenArray® MicroRNA Panels using the QuantStudio™ 12K Flex OpenArray® AccuFill™ System. The plate bottom is encased with an alloy for easy handling. Simply fill with immersion fluid and apply the adhesive lid.
TaqMan® OpenArray® MicroRNA Panels | ||
TaqMan® OpenArray® Real-Time PCR Master Mix
OpenArray® AccuFill™ System The OpenArray® AccuFill™ System loads the sample mix from a 384‐well plate onto the TaqMan® OpenArray® Real-Time PCR plate with minimal human intervention. OpenArray® Sample Tracker Software integrates the sample information from a 384‐well plate file with the assay information contained in the plate setup file. |
Perform a real-time plate read using the QuantStudio™ 12K Flex Real-Time PCR System.
QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block |
A recent study published in Nature found that the QuantStudio 12K Flex system with OpenArray plates outperforms other platforms for miRNA screening.
For Research Use Only. Not for use in diagnostic procedures.