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Real-Time PCR Genetic Variation Analysis Solutions for SARS-CoV-2 Research
Genetic mutations that confer phenotypic changes to viral infectivity and pathogenicity or host immune response and disease outcomes play a vital role in SARS-CoV-2 research. Genetic variations range from single and multi-nucleotide polymorphisms to structural, chromosomal variations, such copy number variations and insertions/deletions. Identifying key genetic variations that play a role in host susceptibility and severity to SARS-COV-2 infection is critical for understanding disease pathogenesis, developing therapeutics, and shaping public health policies.
Sample DNA isolation
Genetic variation analysis requires high-quality genomic DNA. Our nucleic acid purification products are optimized to provide maximum yield, purity, and integrity from virtually any sample type. Select the appropriate product for your sample type and throughput needs.
Learn more ›
TaqMan genetic variation assay selection
Single nucleotide polymorphisms (SNPs) are a common source of genetic variation within genomic DNA. They typically confer a single nucleotide difference within a specific gene locus, resulting in two alleles of the gene. We have expanded our predesigned SNP collection to ensure that we have an assay for every human or mouse genotyping study, including important host SNPs identified from the literature that influence susceptibility and severity of SARS-COV-2 infection. Our portfolio features over 17 million SNP assays, including 4 million HapMap SNPs, nearly 1 million coding SNPs, and 1.4 million common 1000 Genomes SNPs.
Search for predesigned SNP genotyping assays ›
Order custom SNP genotyping assays ›
In addition to single-tubes, predesigned and custom SNP assays are available in the following formats:
- TaqMan Custom Plating Service for 96- or 384-well plates—Order predesigned or custom TaqMan SNP Genotyping assays pre-plated on 96- or 384-well plates. Customize your plate setup to analyze a few SNPs in a large number of samples or a large number of SNPs in just a few samples.
Learn more › - TaqMan OpenArray genotyping plates—Design an OpenArray 384-well plate with your choice of predesigned or custom TaqMan SNP Genotyping assays to generate data for more than 70,000 genotypes in a single day.
Learn more ›
TaqMan SNP Genotyping Assay Sets are pre-defined collections of SNP Genotyping Assays derived from public databases and published articles. Of interest to SARS-CoV-2 therapeutic research, we offer a curated list of relevant host SNPs that influence the course of SARS-CoV-2 infection and clearance, as well as pharmacogenomics-relevant polymorphisms in the cytochrome p450 family of genes.
TaqMan Drug Metabolism (DME) Assays amplify and detect specific SNP alleles, multi-nucleotide polymorphisms (MNPs) and insertion/deletions (InDels) that influence how patients respond to drugs and their risk for side effects, thereby informing patient treatment and management. We offer a collection of 2,700 unique assays to detect polymorphisms in 221 genes that code for various drug metabolism enzymes and drug transporters.
Search for DME assays ›
In addition to single-tubes, DME assays can be ordered in the following formats:
- TaqMan Custom Plating Service for 96- or 384-well plates—Order TaqMan DME assays pre-plated on 96- or 384-well plates. Customize your plate setup to analyze a few SNPs in a large number of samples or a large number of SNPs in just a few samples.
Learn more › - TaqMan OpenArray genotyping plates—Design an OpenArray 384-well plate with your choice of predesigned or custom TaqMan SNP Genotyping assays to generate data for more than 70,000 genotypes in a single day.
Learn more ›
Predesigned panels with TaqMan Drug Metabolism assays
| Panel | Description |
|---|---|
| TaqMan OpenArray PGx Express Panel, QuantStudio 12K Flex | Contains 60 TaqMan DME and SNP genotyping assays. These assays provide coverage of essential, commonly studied markers within CYP2D6, CYP2C9, CYP2C19, and other important DME and clinical research genes. |
| PharmaADME Core Marker Set* | Important variants in Phase I and II metabolism enzymes, transporters, and modifiers in ADME genes |
| Cytochrome P450 Set* | Pharmacogenomics-relevant polymorphisms in the cytochrome p450 family of genes |
*TaqMan SNP Genotyping Assay sets are curated lists of predesigned and custom TaqMan SNP Genotyping assays derived from public databases and published articles. Learn more ›
Copy number variation has implications in phenotypic differences in both healthy and disease states. In particular, the dosage effects from variable copies of key immune response regulators could potentially play a role in disease severity and clearance of pathogen infection. Real-time PCR is the simplest method available to study copy number variation, and TaqMan Copy Number Assays feature easy ordering and tested workflow to enable quick results. In a duplexed reaction, the copy number assay detects the target gene or genomic sequence of interest, and the reference assay detects a sequence that is known to be present in two copies in the diploid genome.
Search for TaqMan Copy Number assays ›
Search for TaqMan Copy Number Reference assays ›
Order Custom or Custom Plus TaqMan Copy Number assays ›
In addition to single tubes, you can order TaqMan Copy Number assays pre-plated in customized configurations on 96- or 384-well plates.
Learn more ›
Select a master mix for genetic variation analysis
We recommend TaqPath ProAmp master mixes, which are general purpose reagents that provide high-performance and high-throughput for genotyping and copy number variation (CNV) applications, including with inhibited samples.
Learn more ›
Explore solutions for every step of the workflow:
TaqPath ProAmp Master Mix
TaqPath ProAmp Multiplex Master Mix
Try our best performing master mixes.
Run real-time PCR and analyze data
We offer real-time PCR instruments for all research needs, from simplicity on a budget to high throughput with maximum productivity.
Learn more ›
Once you order your assays, the protocols and documentation specific to your assay format are provided for download.
Learn more ›
For data analysis, we recommend the Quantstudio Design & Analysis (DA2) app on Connect, our cloud-based platform.
Sample DNA isolation
Genetic variation analysis requires high-quality genomic DNA. Our nucleic acid purification products are optimized to provide maximum yield, purity, and integrity from virtually any sample type. Select the appropriate product for your sample type and throughput needs.
Learn more ›
TaqMan genetic variation assay selection
Single nucleotide polymorphisms (SNPs) are a common source of genetic variation within genomic DNA. They typically confer a single nucleotide difference within a specific gene locus, resulting in two alleles of the gene. We have expanded our predesigned SNP collection to ensure that we have an assay for every human or mouse genotyping study, including important host SNPs identified from the literature that influence susceptibility and severity of SARS-COV-2 infection. Our portfolio features over 17 million SNP assays, including 4 million HapMap SNPs, nearly 1 million coding SNPs, and 1.4 million common 1000 Genomes SNPs.
Search for predesigned SNP genotyping assays ›
Order custom SNP genotyping assays ›
In addition to single-tubes, predesigned and custom SNP assays are available in the following formats:
- TaqMan Custom Plating Service for 96- or 384-well plates—Order predesigned or custom TaqMan SNP Genotyping assays pre-plated on 96- or 384-well plates. Customize your plate setup to analyze a few SNPs in a large number of samples or a large number of SNPs in just a few samples.
Learn more › - TaqMan OpenArray genotyping plates—Design an OpenArray 384-well plate with your choice of predesigned or custom TaqMan SNP Genotyping assays to generate data for more than 70,000 genotypes in a single day.
Learn more ›
TaqMan SNP Genotyping Assay Sets are pre-defined collections of SNP Genotyping Assays derived from public databases and published articles. Of interest to SARS-CoV-2 therapeutic research, we offer a curated list of relevant host SNPs that influence the course of SARS-CoV-2 infection and clearance, as well as pharmacogenomics-relevant polymorphisms in the cytochrome p450 family of genes.
TaqMan Drug Metabolism (DME) Assays amplify and detect specific SNP alleles, multi-nucleotide polymorphisms (MNPs) and insertion/deletions (InDels) that influence how patients respond to drugs and their risk for side effects, thereby informing patient treatment and management. We offer a collection of 2,700 unique assays to detect polymorphisms in 221 genes that code for various drug metabolism enzymes and drug transporters.
Search for DME assays ›
In addition to single-tubes, DME assays can be ordered in the following formats:
- TaqMan Custom Plating Service for 96- or 384-well plates—Order TaqMan DME assays pre-plated on 96- or 384-well plates. Customize your plate setup to analyze a few SNPs in a large number of samples or a large number of SNPs in just a few samples.
Learn more › - TaqMan OpenArray genotyping plates—Design an OpenArray 384-well plate with your choice of predesigned or custom TaqMan SNP Genotyping assays to generate data for more than 70,000 genotypes in a single day.
Learn more ›
Predesigned panels with TaqMan Drug Metabolism assays
| Panel | Description |
|---|---|
| TaqMan OpenArray PGx Express Panel, QuantStudio 12K Flex | Contains 60 TaqMan DME and SNP genotyping assays. These assays provide coverage of essential, commonly studied markers within CYP2D6, CYP2C9, CYP2C19, and other important DME and clinical research genes. |
| PharmaADME Core Marker Set* | Important variants in Phase I and II metabolism enzymes, transporters, and modifiers in ADME genes |
| Cytochrome P450 Set* | Pharmacogenomics-relevant polymorphisms in the cytochrome p450 family of genes |
*TaqMan SNP Genotyping Assay sets are curated lists of predesigned and custom TaqMan SNP Genotyping assays derived from public databases and published articles. Learn more ›
Copy number variation has implications in phenotypic differences in both healthy and disease states. In particular, the dosage effects from variable copies of key immune response regulators could potentially play a role in disease severity and clearance of pathogen infection. Real-time PCR is the simplest method available to study copy number variation, and TaqMan Copy Number Assays feature easy ordering and tested workflow to enable quick results. In a duplexed reaction, the copy number assay detects the target gene or genomic sequence of interest, and the reference assay detects a sequence that is known to be present in two copies in the diploid genome.
Search for TaqMan Copy Number assays ›
Search for TaqMan Copy Number Reference assays ›
Order Custom or Custom Plus TaqMan Copy Number assays ›
In addition to single tubes, you can order TaqMan Copy Number assays pre-plated in customized configurations on 96- or 384-well plates.
Learn more ›
Select a master mix for genetic variation analysis
We recommend TaqPath ProAmp master mixes, which are general purpose reagents that provide high-performance and high-throughput for genotyping and copy number variation (CNV) applications, including with inhibited samples.
Learn more ›
Explore solutions for every step of the workflow:
TaqPath ProAmp Master Mix
TaqPath ProAmp Multiplex Master Mix
Try our best performing master mixes.
Run real-time PCR and analyze data
We offer real-time PCR instruments for all research needs, from simplicity on a budget to high throughput with maximum productivity.
Learn more ›
Once you order your assays, the protocols and documentation specific to your assay format are provided for download.
Learn more ›
For data analysis, we recommend the Quantstudio Design & Analysis (DA2) app on Connect, our cloud-based platform.
For Research Use Only. Not for use in diagnostic procedures.