High Capacity RNA-to-cDNA Products | Thermo Fisher Scientific

High-Capacity cDNA Reverse Transcription Kit product photo

True to its name, the High-Capacity cDNA synthesis kits produce high quality first-strand cDNA from total RNA. The High-Capacity cDNA Reverse Transcription Kit (formerly the High-Capacity cDNA Archive Kit) is designed for synthesizing cDNA for short- or long-term archiving. The kits yield quantitative reverse transcription products from RNA inputs ranging between 0.02 and 2 μg in a 20 μL reaction; it can be scaled up to 10 μg of input RNA in a 100 μL reaction. Downstream applications include real-time PCR, standard PCR, and labeling for microarray analysis.

High-capacity cDNA synthesis kits: formats

The High-Capacity cDNA reverse transcription kits are available in multiple formats:

Benefits of the High-Capacity RNA-to-cDNA Kit

The High-Capacity RNA-to-cDNA kit consolidates the reagents for reverse transcription reaction into two tubes: 2X RT buffer mix and 20X enzyme mix, which contains reverse transcriptase and RNase inhibitor. These components have been optimized to provide high cDNA yields for real-time gene expression analysis. The two-tube format streamlines the workflow by minimizing the number of pipetting steps and is designed for optimum performance in real-time PCR with TaqMan Fast Advanced Master Mix and PowerUp SYBR Green Master Mix.

Comparison of cDNA synthesis kits for qPCR

For exceptional performance, explore SuperScript IV Reverse Transcriptase—available as a standalone enzyme, first-strand cDNA synthesis kit, master mix for qRT-PCR applications, and one-step RT-PCR system to suit all your research needs.

Reverse transcriptases: Selection tool

Use the selection tool to find the right product for your cDNA synthesis experiments

	High-Capacity cDNA Reverse Transcription kit	High-Capacity RNA-to-cDNA kit	SuperScript IV VILO Master Mix	SuperScript IV VILO Master Mix with ezDNase Enzyme
Application	Value choice for routine applications	cDNA synthesis components in two-tube format	Exceptional master mix for two-step RT-qPCR	Exceptional master mix for two-step RT-qPCR with fast and easy gDNA removal
Reverse transcriptase	MultiScribe	MultiScribe	SuperScript IV	SuperScript IV
Optimal reaction temperature	37°C	37°C	50°C	50°C
Reaction time	120 min	30 to 60 min	10 min	10 min
Ability to work with degraded or inhibitor-containing RNA	Low	Low	High	High
Reduced RNase H activity	No	No	Yes	Yes
Primers included	Random primers	Random primers	Random primers and oligo(dT)₂₀	Random primers and oligo(dT)₂₀
Integrated gDNA removal	No	No	No	Yes*
Format	RT reaction components in separate tubes With RNase inhibitor Without RNase inhibitor	RT reaction components in two-tube format	All RT reaction components premixed	All RT reaction components premixed

*Formats supplied with ezDNase enzyme

Resources

Reverse transcriptase selection tool
Find the right products to fit your cDNA synthesis needs.

Reverse transcriptase webinars
Stay up-to-date on current industry trends in cDNA synthesis applications with on-demand webinars.

Reverse transcriptase literature
Access white papers and technical and application notes for insights on cutting edge reverse transcription technology.

Reverse transcriptase videos
Watch instructional videos on troubleshooting tips, how-to videos, and explainers related to cDNA synthesis.

Reverse transcriptase education
Review educational resources on topics including reverse transcription basics, cDNA synthesis, enzyme selection, troubleshooting tips, and cDNA applications in molecular biology.

Support

PCR and cDNA Synthesis Support Center
Find support for every step of your cDNA synthesis experiments.

T_m calculator
Handy tool to calculate T_m (melting temperature) of primers and annealing temperatures.

Contact us
Get in touch with our technical application scientists for questions regarding reverse transcription enzymes, master mixes, and cDNA synthesis kits.

Mobile and desktop applications
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OEM and custom solutions
Source reverse transcriptase and other molecular biology enzymes, PCR reagents, and plastics customized to your needs.

For Research Use Only. Not for use in diagnostic procedures.