Proteases and Protein-Cleaving Reagents

We offer purified and agarose-immobilized proteases for enzymatic proteolysis (cleavage or digestion) of proteins to facilitate amino acid sequencing, peptide analysis, and polypeptide structural characterization.

For protein digestion using mass spectrometry, we offer a wide range of highly purified and MS-validated proteases.

Choose the right protease for your application

Fusion tag removal
EnzymePurified proteaseImmobilized proteasesSpecificity
Factor Xa32521
32520		N/ACleaves at the carboxyl side of Ile-Glu-Gly-Arg sequence
Human Rhinovirus 3C Protease
(HRV 3C)		88946N/AHighly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue
S. aureus V-8 Protease2019520151Cleaves at the carboxyl side of aspartate and glutamate residues
Antibody fragmentation
EnzymePurified proteaseImmobilized proteasesSpecificity
FicinN/A44881Cleaves non-specifically at exposed residues, favors aromatic residues
PapainN/A20341Cleaves nonspecifically at exposed residues
PepsinN/A20343Cleaves nonspecifically at exposed residues, favors aromatic residues
Proteomics using MS Analysis
EnzymePurified proteaseImmobilized proteasesSpecificity
Trypsin, MS Grade90057
90058
90059
90305		N/ACleaves at the carboxyl side of arginine and lysine residues
Lys-C Protease, MS Grade90051N/ACleaves at the carboxyl side of lysine residues
Trypsin/Lys-C Protease Mix, MS GradeA41007N/ACleaves at the carboxyl side of lysine residues
Lys-N Protease, MS Grade90300
90301		N/ACleaves at the amino side of lysine residues
Asp-N Protease, MS Grade90053N/ACleaves at the amino side of aspartate residues
Chymotrypsin, MS Grade90056N/ACleaves at the carboxyl side of tyrosine, phenylalanine, tryptophan, and leucine
Glu-C Protease, MS Grade90054N/ACleaves at the carboxyl side of glutamate or aspartate
Adherent cells dissociation
EnzymePurified proteaseImmobilized proteasesSpecificity
Trypsin (TPCK)2023320230Cleaves at the carboxyl side of arginine and lysine residues
DNase/RNase deactivation
EnzymePurified proteaseImmobilized proteasesSpecificity
Proteinase K17916N/ACleaves at the carboxyl side of aliphatic, aromatic, and hydrophobic residues

Resources

For Research Use Only. Not for use in diagnostic procedures.