Silencer Select siRNA Libraries | Thermo Fisher Scientific

High throughput screening with multiwell plates

Improved specificity for high-confidence screening results

Silencer Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity.

Improve RNAi screening results with highly efficient, specific siRNAs

The benefits of Silencer Select siRNAs are amplified in the context of high-throughput screening applications. Trust your discovery to the siRNA with our knockdown guarantee in the industry!

Attributes	Advantages
Advanced bioinformatic seed region analysis for sequence selection LNA chemical modifications	Reduced off-target effects by up to 90% for fewer false positives Less toxicity due to off-target effects More consistent phenotypic data
Next-generation design algorithm	Up to 100-fold more potent silencing than other available siRNAs Improved silencing for more robust knockdown results Fewer false negatives for greater experimental confidence
Three unique individual siRNAs per gene	Multiple data points per gene for rapid progression from primary screening to hit confirmation studies

If you’re ready to start screening with a pre-defined collection or want to request a custom library, request a quote now!

Request a quote

Pre-defined Silencer Select siRNA libraries

These libraries have been pre-assembled using PANTHER (www.pantherdb.org ) and Gene Ontology™ annotation information to create up-to-date target gene sets.

Each gene is targeted by three unique siRNAs. Validated siRNAs are included at no extra cost.
0.25 nmol of each siRNA is provided dried-down in the wells of barcoded 96- or 384-well plates. This is sufficient for 100-500+ transfections, depending on siRNA working concentration and reaction volume.
Column 12 of each 96-well plate and columns 23 and 24 in each 384-well plate are left empty to allow for the addition of controls at the time of use.
Plates are conveniently arranged for pooling of siRNA, if desired.
The libraries are delivered with plate map files including full siRNA sequence information, as well as each targeted gene's symbol, full name, aliases, RefSeq accession, and Entrez Gene ID.

If you would like more information on any of the libraries listed below, click the product link or email us at RNAisupport@thermofisher.com

Custom Silencer Select siRNA libraries

Silencer Select siRNAs are available as custom collections to suit your specific needs. These highly economical plated versions of our guaranteed siRNAs are perfect for pilot studies, primary screen follow-up, or a customized functional genomics screen.

Minimum order of 20 siRNAs
Up to 3 siRNAs per gene
Choose from 96-well, 384-well, or 384-well Echo-qualified plates
Validated siRNAs are available at no additional cost!

Product line for custom siRNA libraries	Quantity per well*
Silencer Select human pre-designed inventoried siRNA	0.1, 0.25, 1, or 2 nmol
*NOTE: Not all quantities available in 384-well

Request a quote for a custom siRNA library

Publications featuring Silencer Select siRNA libraries

Since 2010, Silencer Select siRNA libraries have been enabling functional genomics studies in labs around the world. These selected peer-reviewed references showcase the power of our high-quality siRNAs in high-throughput screening applications.

Lingeman M, McCarty T, et al. (2019) The alpha-1 subunit of the Na+,K+-ATPase (ATP1A1) is required for macropinocytic entry of respiratory syncytial virus (RSV) in human respiratory epithelial cells. PLoS Pathog 15: e1007963.
A genome-wide Silencer Select siRNA library screen uncovers a model for RSV entry into human airway epithelial cells requiring activation of ATP1A1, signaling it as a potential new target for the development of anti-RSV drugs.
Hasson S, Kane L, et al. (2013) High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy. Nature 504: 291-295.
This Nature publication demonstrates the utility of the Silencer Select genome-wide siRNA library for arrayed high-throughput screening in effectively identifying novel drug targets for Parkinson’s disease.
Shum D, Bhinder B, et al. (2013) An arrayed RNA interference genome-wide screen identifies candidate genes involved in the MicroRNA 21 biogenesis pathway. Assay Drug Dev Technol 11: 191-205.
Researchers at Memorial Sloan Kettering uncover a number of novel candidate regulators of microRNA function using the Silencer Select whole genome siRNA library.
Sivan G, Ormanoglu P, et al. (2015) Identification of Restriction Factors by Human Genome-Wide RNA Interference Screening of Viral Host Range Mutants Exemplified by Discovery of SAMD9 and WDR6 as Inhibitors of the Vaccinia Virus K1L⁻C7L⁻ Mutant. mBio 6: e01122-15.
Genome-wide screen results demonstrate higher phenotypic penetrance from multiple individual Silencer Select siRNAs than with a pooled siRNA reagent from another vendor.
Sanderlin A, Vondrak C, et al. (2019) RNAi screen reveals a role for PACSIN2 and caveolins during bacterial cell-to-cell spread. Mol Biol Cell 30: 2124-2133.
A custom siRNA library targeting 115 host genes was applied to study the effects of L. monocytogenes bacterial spread.

Pre-defined collections

Pre-defined Silencer Select siRNA libraries

These libraries have been pre-assembled using PANTHER (www.pantherdb.org ) and Gene Ontology™ annotation information to create up-to-date target gene sets.

Each gene is targeted by three unique siRNAs. Validated siRNAs are included at no extra cost.
0.25 nmol of each siRNA is provided dried-down in the wells of barcoded 96- or 384-well plates. This is sufficient for 100-500+ transfections, depending on siRNA working concentration and reaction volume.
Column 12 of each 96-well plate and columns 23 and 24 in each 384-well plate are left empty to allow for the addition of controls at the time of use.
Plates are conveniently arranged for pooling of siRNA, if desired.
The libraries are delivered with plate map files including full siRNA sequence information, as well as each targeted gene's symbol, full name, aliases, RefSeq accession, and Entrez Gene ID.

If you would like more information on any of the libraries listed below, click the product link or email us at RNAisupport@thermofisher.com

Custom collections

Custom Silencer Select siRNA libraries

Minimum order of 20 siRNAs
Up to 3 siRNAs per gene
Choose from 96-well, 384-well, or 384-well Echo-qualified plates
Validated siRNAs are available at no additional cost!

Product line for custom siRNA libraries	Quantity per well*
Silencer Select human pre-designed inventoried siRNA	0.1, 0.25, 1, or 2 nmol
*NOTE: Not all quantities available in 384-well

Request a quote for a custom siRNA library

Publications

Publications featuring Silencer Select siRNA libraries

Lingeman M, McCarty T, et al. (2019) The alpha-1 subunit of the Na+,K+-ATPase (ATP1A1) is required for macropinocytic entry of respiratory syncytial virus (RSV) in human respiratory epithelial cells. PLoS Pathog 15: e1007963.
A genome-wide Silencer Select siRNA library screen uncovers a model for RSV entry into human airway epithelial cells requiring activation of ATP1A1, signaling it as a potential new target for the development of anti-RSV drugs.
Hasson S, Kane L, et al. (2013) High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy. Nature 504: 291-295.
This Nature publication demonstrates the utility of the Silencer Select genome-wide siRNA library for arrayed high-throughput screening in effectively identifying novel drug targets for Parkinson’s disease.
Shum D, Bhinder B, et al. (2013) An arrayed RNA interference genome-wide screen identifies candidate genes involved in the MicroRNA 21 biogenesis pathway. Assay Drug Dev Technol 11: 191-205.
Researchers at Memorial Sloan Kettering uncover a number of novel candidate regulators of microRNA function using the Silencer Select whole genome siRNA library.
Sivan G, Ormanoglu P, et al. (2015) Identification of Restriction Factors by Human Genome-Wide RNA Interference Screening of Viral Host Range Mutants Exemplified by Discovery of SAMD9 and WDR6 as Inhibitors of the Vaccinia Virus K1L⁻C7L⁻ Mutant. mBio 6: e01122-15.
Genome-wide screen results demonstrate higher phenotypic penetrance from multiple individual Silencer Select siRNAs than with a pooled siRNA reagent from another vendor.
Sanderlin A, Vondrak C, et al. (2019) RNAi screen reveals a role for PACSIN2 and caveolins during bacterial cell-to-cell spread. Mol Biol Cell 30: 2124-2133.
A custom siRNA library targeting 115 host genes was applied to study the effects of L. monocytogenes bacterial spread.

Resources

Questions?

Technical inquires:
Our Technical Application Scientists are available to help assist you at techsupport@thermofisher.com

Ordering & Order Status inquires:
If you have questions about pre-designed RNAi orders and order status, please contact us at genomicorders@thermofisher.com

If you have any questions about Custom RNAi orders and order status, please contact us at RNAiSupport@thermofisher.com

For Research Use Only. Not for use in diagnostic procedures.