Epigenomic information is important in understanding how the function of the genetic sequence is implemented and regulated.  Since the epigenome is less stable than the genome, it is thought to be important in gene-environment interactions.  However, being less stable, the epignome exhibits more variablity that the genome, making epigenomic mapping inherently more complex.

Step-by-step guide to methylation analysis sample prep products

For all next-generation sequencing platforms

MagMAX FFPE DNA/RNA Ultra Kit
Designed for sequential isolation of DNA and RNA from the same formaldehyde- or paraformaldehyde-fixed paraffin-embedded (FFPE) tissue sample.

PureLink Genomic DNA Mini Kit
Recommended for next generation sequencing applications requiring extraction and purification of genomic DNA samples.  Yields high-quality DNA in a single column.

For all next-generation sequencing platforms

MethylCode Bisulfite ConversionKit
Fast and easy way to convert unmethylated cytosines from a DNA sample into uracils while methylated cytosines remain unchanged. The modified DNA is ideal for PCR amplification for downstream analyses, including restriction endonuclease digestion, sequencing and microarrays.

MethylMiner Methylated DNA Conversion Kit
Enrichment & differential fractionation of dsDNA by CpG methylation density.  Increased sensitivity over antibody methods.

Cells-to-CpG Bisulfite Conversion Kit
Fast & easy conversion of unmethylated cytosines into uracils while methylated cytosines remain unchanged, starting directly from cells, tissue, blood, FFPE, or purified DNA

For all next-generation sequencing platforms

Ion AmpliSeq Library Kit Plus
Designed for rapid preparation of amplicon libraries using Ion AmpliSeq panels.

Ion AmpliSeq Kit for Chef DL8
Designed for use with the Ion Chef System for automation of Ion AmpliSeq library construction.

Ion Chef System
Provides an automated, walk-away workflow solution for Ion AmpliSeq library preparation and reproducible template preparation and chip loading. Simplifies workflow for Ion GeneStudio S5 series, Ion PGM, and Ion Proton systems.

Ion 520 & Ion 530 ExTKit-Chef
Enables robust, automated, and fast template preparation and sequencing of 400 to 600 base-read libraries using the Ion Chef System and the Ion S5 and Ion S5 XL Sequencing Systems.

E-Gel SizeSelect Gels
Rapid size separation and recovery system for library construction.

Ion GeneStudio Systems
Flexibly designed to enable a broad range of targeted NGS applications with industry-leading speed and scalability.

Ion sequencing chips
Select from various sequencing chips to sequence a throughput range from 2M to 130M reads per run.

Analyze sequencing output from the Ion AmpliSeq Methylation Panel for Cancer Research using Torrent Suite and Torrent Suite plugin under the name “methylation_analysis”, available in the Themo Fisher Scientific Plugin store.

Step 1 : DNA extraction

For all next-generation sequencing platforms

MagMAX FFPE DNA/RNA Ultra Kit
Designed for sequential isolation of DNA and RNA from the same formaldehyde- or paraformaldehyde-fixed paraffin-embedded (FFPE) tissue sample.

PureLink Genomic DNA Mini Kit
Recommended for next generation sequencing applications requiring extraction and purification of genomic DNA samples.  Yields high-quality DNA in a single column.

Step 2 : Enrichment

For all next-generation sequencing platforms

MethylCode Bisulfite ConversionKit
Fast and easy way to convert unmethylated cytosines from a DNA sample into uracils while methylated cytosines remain unchanged. The modified DNA is ideal for PCR amplification for downstream analyses, including restriction endonuclease digestion, sequencing and microarrays.

MethylMiner Methylated DNA Conversion Kit
Enrichment & differential fractionation of dsDNA by CpG methylation density.  Increased sensitivity over antibody methods.

Cells-to-CpG Bisulfite Conversion Kit
Fast & easy conversion of unmethylated cytosines into uracils while methylated cytosines remain unchanged, starting directly from cells, tissue, blood, FFPE, or purified DNA

Step 3 : Library and template preparation

For all next-generation sequencing platforms

Ion AmpliSeq Library Kit Plus
Designed for rapid preparation of amplicon libraries using Ion AmpliSeq panels.

Ion AmpliSeq Kit for Chef DL8
Designed for use with the Ion Chef System for automation of Ion AmpliSeq library construction.

Ion Chef System
Provides an automated, walk-away workflow solution for Ion AmpliSeq library preparation and reproducible template preparation and chip loading. Simplifies workflow for Ion GeneStudio S5 series, Ion PGM, and Ion Proton systems.

Ion 520 & Ion 530 ExTKit-Chef
Enables robust, automated, and fast template preparation and sequencing of 400 to 600 base-read libraries using the Ion Chef System and the Ion S5 and Ion S5 XL Sequencing Systems.

E-Gel SizeSelect Gels
Rapid size separation and recovery system for library construction.

Step 4 : Run sequence

Ion GeneStudio Systems
Flexibly designed to enable a broad range of targeted NGS applications with industry-leading speed and scalability.

Ion sequencing chips
Select from various sequencing chips to sequence a throughput range from 2M to 130M reads per run.

Step 5 : Analyze data

Analyze sequencing output from the Ion AmpliSeq Methylation Panel for Cancer Research using Torrent Suite and Torrent Suite plugin under the name “methylation_analysis”, available in the Themo Fisher Scientific Plugin store.

Resources

Support

For Research Use Only. Not for use in diagnostic procedures.