AmpliSeq HD Showcase
Custom library amplification technology uniquely sensitive to your needs

Ion AmpliSeq HD technology is a new amplicon-based library preparation technology for Ion Torrent next-generation sequencing (NGS) that gives you the power to design your own panels and find variants with a low limit of detection—down to 0.1% for cell-free DNA samples. This revolutionary technology enables researchers to routinely get answers when ultrahigh sensitivity is required, such as when detecting low-frequency alleles in circulating tumor DNA or trace pathogenic microbial species in blood.

Combined with the Ion GeneStudio S5 sequencers, Ion AmpliSeq HD panels offer a flexible targeted-NGS solution for:

  • Cancer research
  • Infectious disease research
  • Microbial studies
  • Gene expression studies

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Custom gene panel webinars

Researchers share how ultrahighly sensitive custom gene panels are applied in their research in oncology and human genetic disease.

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How does Ion AmpliSeq HD technology work?

Ion AmpliSeq HD technology leverages unique molecular tags, or UMTs, and the made-to-order design pipeline in Ion AmpliSeq Designer to help deliver results with ultrahigh sensitivity.

Watch the video below to learn more about this novel technology.


Unmatched sensitivity for your research

As genetic research rapidly evolves, the ability to confidently detect low-frequency variants or quantitate transcript-level expression is becoming more critical to the development of NGS assays with ultrahigh sensitivity. Ion AmpliSeq HD panels have been shown to achieve a 0.1% limit of detection for cell-free DNA samples, with >99% specificity for SNVs, hotspots, indels, CNVs, and fusions. Ion AmpliSeq HD panels support both DNA and RNA samples and require as little as 1 ng nucleic acids input from FFPE samples.

LOD data
Figure 1. Amplicon coverage and input material determine limit of detection using Ion AmpliSeq HD panels.

Ion Torrent next-generation sequencing systems

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Scalable targeted NGS to support small and large projects
The Ion GeneStudio S5 system is a scalable, targeted-NGS workhorse with wide application breadth and throughput capability.

Ion Torrent Genexus System

Specimen to report in a single day with a hands-off, automated workflow*
The Genexus System is the first turnkey NGS solution that automates the specimen-to-report workflow and delivers results in a single day with just two user touchpoints.* 

 Poster: A rapid library preparation method with custom assay designs for detection of variants at 0.1% allelic frequency in liquid biopsy samples