Collibri NGS Whole-Genome Library Prep Kits

Collibri DNA Whole Genome Sequencing Kits

Invitrogen Collibri whole-genome library prep kits improve library prep success for high-throughput Illumina™ next-generation sequencing (NGS) systems.

PCR-free protocols
Best coverage of challenging genomic regions with the physical shearing protocol
Improved sensitivity of variant detection from 1 ng of DNA compared to Illumina, Kapa Biosystems™, or NEB™ kits
Visual feedback for higher library prep success rates
Protocols available for DNA that is physically sheared (PS) or enzymatically sheared (ES)

Compare options Request sample

Accommodates a wide range of sample types and inputs:

Intact DNA
Degraded DNA, including FFPE or cfDNA
Suitable for genomes of all sizes
Consistent gene coverage from 1 to 500–1,000 ng

Variant detection is consistent among low abundance (1 ng) and high abundance (500–1,000 ng) samples to confidently analyze variable samples.

Sequence SARS-CoV-2 samples

Miniaturize Collibri NGS library prep kits

High-quality genomic coverage from nasopharyngeal, oropharyngeal, and diverse samples increases accuracy in SARS-CoV-2 research such as the study of viral evolution and transmission patterns. The optimized workflow for SARS-CoV-2 reduces time and sequencing costs while providing flexibility to use available reagents.

Review the optimized protocol ›

Physically sheared DNA

Sequencing studies can now accept a greater diversity of samples and retain confidence in genomic interpretation. Variant detection rates by the Invitrogen Collibri PS DNA Library Prep Kits for Illumina Systems are similar between amplified (1–100 ng) libraries and PCR-free (500–1,000 ng) libraries.

Figure 1. Coriell NA12878 DNA was converted into a library and sequenced on an Illumina™ NovaSeq™ 6000 system at 372M reads per sample. SNP recall calculated using FreeBayes v1.2.0.

Enzymatically sheared DNA

Consistency of variant detection provides more consistent, predictable results from diverse samples. Variant detection rates by the Invitrogen Collibri ES DNA Library Prep Kits for Illumina Systems are similar between amplified (100 ng) libraries and PCR-free (100 ng) libraries.

Figure 2. Coriell NA12878 DNA was converted into a library and sequenced on an Illumina NovaSeq 6000 system at 258M reads per sample. SNP recall calculated using FreeBayes v1.2.0.

Improve statistical power of NGS results through miniaturization

“…in a 1/10th reaction with Collibri preps, you can see really tight performance.”
Dr. Stuart Levine, Director of the BioMicro Center, MIT

Watch presentation of results ›

Product comparison

	Collibri PS DNA Library Prep Kits for Illumina Systems	Collibri ES DNA Library Prep Kits for Illumina Systems
PCR-free input	500 ng	100 ng
Input	1–1,000 ng	1–500 ng
Lowest GC bias
DNA shearing method	Physical shearing (e.g., Covaris)	Enzymatic shearing
Species supported	All	All
PCR-free assay time (hr)	1.5	1.5
Assay time (hr)	2.75	2.5
PCR-free hands-on time (hr)	0.5	0.5
Hands-on time (hr)	1	1
Target insert size (bp)	350-550	350-550
All components included
Visual feedback
	View performance data	View performance data