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Gateway Recombination and Seamless Cloning Support
Explore our “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through our "Guides and Tools" section to access a comprehensive portfolio of product-related support resources.
Getting Started
- What is the difference between BP Clonase and BP Clonase II and between LR Clonase and LR Clonase II?
- How do I shuttle my gene from the Destination vector it is currently in to a new Destination vector?
- What is the difference between the MultiSite Gateway Pro kit and the MultiSite Gateway 3-Fragment Vector Construction Kit? Are the kits compatible with each other?
- What is the difference between the GeneArt Seamless PLUS Cloning and Assembly Kit and the original GeneArt Seamless Cloning and Assembly Kit?
- I see that you offer TOPO, Gateway, and the new GeneArt Assembly kits and systems for cloning. What should I use?
Troubleshooting
- I performed a BP reaction and got few or no colonies after transformation, whereas the transformation control gave colonies.
- I performed an LR reaction and got few or no colonies after transformation, whereas the transformation control gave colonies.
- I performed a BP reaction and got no colonies after transformation and the recombination positive control was not successful.
- I performed an LR reaction and got no colonies after transformation and the recombination positive control was not successful.
- I performed a BP reaction and got two distinct types of colonies (large and small) after transformation.
Guides and Tools
- Search our database for Product Documents: Manuals, Vector Maps, Citations and References
- Search our database for Quality and Safety Documents: MSDSs, COAs
General
Gateway Cloning
- Overview: Gateway Cloning Technology
- Overview: Gateway Cloning Comes of Age (Exclusive Interview)
- Tutorial: Gateway Cloning Technology Online Tutorial
MultiSite Gateway Cloning
- Overview: MultiSite Gateway Technology
GeneArt Seamless Cloning and Assembly
- Overview: GeneArt Seamless Cloning and Assembly
- Overview: GeneArt Type IIs Assembly Kits
- Selection Table: GeneArt Seamless Cloning and Assembly kit
- Vector Selection Tool
- GeneArt Design Tool: facilitates the in silico design, assembly, or mutagenesis of a DNA molecule using GeneArt technology
Custom Services
Gateway Vector Conversion
The Gateway Vector Conversion service includes: 1) subcloning of a Gateway cassette into your vector of interest to create a Gateway destination vector, 2) validation of the vector for LR recombination, and 3) delivery of the vector as a glycerol stock.
Custom Gene Synthesis
Gene synthesis outperforms traditional cloning by providing fast turnaround times and guaranteed sequence accuracy. Construct design and optimization services are also available in order to fine tune the expression of your gene(s) of interest.
Plasmid Services
GeneArt Plasmid Services include flexible offerings covering construction of plasmid vectors customized for your needs, and subcloning of your sequence into any vector, including the Gateway vector system. Concentrate on your research goals and leave your plasmid construction and subcloning work to us.
For Research Use Only. Not for use in diagnostic procedures.