Posters

2015

Use of Cryopreserved Plateable Hepatocytes in Conjunction with Hepextend™

Optimization of Primary Hepatocyte Media for Evaluation of Drug Metabolism and Drug-Induced Liver Injury

Isolation and Characterization of Quiescent Human Hepatic Stellate Cells For Physiological Modeling of Liver Metabolism and Function

2014

Comparative Analysis of DILI Compounds on Cytokine Production in Kupffer Cells and Hepatocytes

A DIY Model for Generation of Robust 3D Epidermal Skin Equivalents Composed of Normal Human Primary Epidermal Keratinocytes

Reproducible Generation of 3D Corneal Models from Normal Human Limbal Derived Progenitor Cells

Long Term Hepatocyte Culture for Drug Safety Assessment Screening

In Vitro Modeling of Inflammatory Response Associated with Cholestatic Injury in HepaRG

2012

Optimization of a Plated Cryopreserved Human Hepatocyte Model for Assessment of the Metabolic Stability of Low Clearance Compounds

Enzyme Activity and Transporter Facilitated Uptake Comparisons: Multi-donor Pooled Cryopreserved Hepatocytes (HEP10™) to Single Donor Constituent Lots

Modeling of Cytokine-Induced Liver Hepatotoxicity of Trovafloxacin Using Co-cultures of Hepatocytes and Kupffer Cells

2011

Cryopreserved HepaRG Cells An Alternative In Vitro Screening Tool for Human Hepatic Drug Metabolism

Use of Cryopreserved Primary Rat and Human Kupffer Cells and Hepatocytes in Co-cultures to Study APAP Cytotoxicity

Long Term Storage Effects on the Metabolic Activity of Cryopreserved Primary Human and Rat Hepatocytes

2010

Development and Testing of Cryopreserved Carp Hepatocytes for the Estimation of Bioconcentration in Fish

In Vitro Metabolic Enzyme Activity Characterization in Cryopreserved, Freshly Isolated Hepatocytes and Liver S9 Fraction from Rainbow Trout

In Vitro Fish Metabolism and Metabolite Profiling of Several Fragrances using Rainbow Trout Liver S9 Fraction and Cryopreserved Hepatocytes

Taurocholate or Glycocholate – Which is the More Appropriate Substrate for Assessing Hepatic Bile Salt Transport?

Influence of Gender and Ethnicity on Drug Metabolizing Enzymes in Primary Human Hepatocytes

Utilization and Optimization of Cryopreserved Human Hepatocytes as a Model to Assess CYP450 Inhibition

Comparison of Metabolic Capacities of Fresh and Cryopreserved Human Hepatocytes Isolated from the Same Donor: Metabolic Stability, Plated Metabolism, Metabolite ID Applications

Induction of Transporter Expression and Modulation of Hepatobiliary Disposition using Sandwich Cultures of Human Hepatocytes

Genotyped, Cryopreserved Suspensions of Human Hepatocytes as a Tool for Investigating Drug Metabolism in Polymorphic Individuals

Suppression of OATP1B1, OATP1B3, and OATP2B1 Transporters in Primary Cryopreserved Human Hepatocytes Following Lipid Delivery of Stealth siRNA™

Time-Course Disposition of Ritonavir and Prototypical Hepatic Inducers in Cultures of Primary Human Hepatocytes: Context for Induction & Inhibition Concentration Responses

Publications

DDI Induction | Environmental Tox | Toxicology | Drug Metabolism |  Hepatocyte Cell Bio | Co-cultures

 

DDI Induction

 

Li Y, Zhou D, Ferguson SS, Dorff P, Simpson TR, Grimm SW. In Vitro Assessment of Metabolic Drug–Drug Interaction Potential of AZD2624, Neurokinin-3 Receptor Antagonist, Through Cytochrome P(450) Enzyme Identification, Inhibition, and Induction Studies. Xenobiotica. 2010 Nov;40(11):721-9.

Li L, Hassan HE, Tolson AH, Ferguson SS, Eddington ND, Wang H. Differential Activation of Pregnane X Receptor and Constitutive Androstane Receptor by Buprenorphine in Primary Human Hepatocytes and HepG2 Cells. J Pharmacol Exp Ther. 2010 Sep.

 

Rana R, Chen Y, Ferguson SS, Kissling GE, Surapureddi S. Hepatocyte Nuclear Factor 4alpha Regulates Rifampicin-Mediated Induction of CYP2C Genes in Primary Cultures of Human Hepatocytes. Drug Metab Dispos. 2010 Apr;38(4):591-9.

 

Lau AJ, Yang G, Rajaraman G, Baucom CC, Chang TK. Differential Effect of Meclizine on the Activity of Human Pregnane X Receptor and Constitutive Androstane Receptor. J Pharmacol Exp Ther. 2010 Dec.

Lau AJ, Yang G, Rajaraman G, Baucom CC, Chang TK. Human Pregnane X Receptor Agonism by Ginkgo Biloba Extract: Assessment of the Role of Individual Ginkgolides. J Pharmacol Exp Ther. 2010 Dec;335(3):771-80.

Graham RA, Tyler LO, Krol WL, Silver IS, Webster LO. Temporal Kinetics and Concentration-Response Relationships for Induction of CYP1A, CYP2B, and CYP3A in Primary Cultures of Beagle Dog Hepatocytes. J Biochem Mol Toxicol. 2006;20(2):69-78.

Faucette SR, Sueyoshi T, Smith CM, Negishi M, Lecluyse EL. Differential Regulation of Hepatic CYP2B6 and CYP3A4 Genes by Constitutive Androstane Receptor but Not Pregnane X Receptor. J Pharmacol Exp Ther. 2006 Jun;317(3):1200-9.

Ferguson SS, Chen Y, LeCluyse EL, Negishi M, Goldstein JA. Mol Pharmacol. Human CYP2C8 is Transcriptionally Regulated by the Nuclear Receptors Constitutive Androstane Receptor, Pregnane X Receptor, Glucocorticoid Receptor, and Hepatic Nuclear Factor 4alpha. 2005 Sept;68(3):747-57.

Smith CM, Graham RA, Krol WL, Silver IS, Negishi M. Differential UGT1A1 Induction by Chrysin in Primary Human Hepatocytes and HepG2 Cells. J Pharmacol Exp Ther. 2005 Dec;315(3):1256-64.

Madan A, Graham RA, Carroll KM, Mudra DR, Burton LA. Effects of Prototypical Microsomal Enzyme Inducers on Cytochrome P450 Expression in Cultured Human Hepatocytes. Drug Metab Dispos. 2003 Apr;31(4):421-31.

Wang H, LeCluyse EL. Role of Orphan Nuclear Receptors in the Regulation of Drug-Metabolising Enzymes. Clin Pharmacokinet. 2003;42(15):1331-57.

LeCluyse EL. Pregnane X Receptor: Molecular Basis for Species Differences in CYP3A Induction by Xenobiotics. Chem Biol Interact. 2001 May 16;134(3):283-9.

LeCluyse E, Madan A, Hamilton G, Carroll K, DeHaan R. Expression and Regulation of Cytochrome P450 Enzymes in Primary Cultures of Human Hepatocytes. J Biochem Mol Toxicol. 2000;14(4):177-88.

McCune JS, Hawke RL, LeCluyse EL, Gillenwater HH, Hamilton G. In Vivo and In Vitro Induction of Human Cytochrome P4503A4 by Dexamethasone. Clin Pharmacol Ther. 2000 Oct;68(4):356-66.

 

Environmental Tox

 

Budinsky RA, LeCluyse EL, Ferguson SS, Rowlands JC, Simon T. Human and Rat Primary Hepatocyte CYP1A1 and 1A2 Induction with 2,3,7,8-Tetrachlorodibenzo-p-dioxin, 2,3,7,8-Tetrachlorodibenzofuran, and 2,3,4,7,8-Pentachlorodibenzofuran. Toxicol Sci. 2010 Nov;118(1):224-35.

Rotroff D, Beam A, Freeman K, Farmer A, Bondell H. Xenobiotic Metabolizing Enzyme and Transporter Gene Expression in Primary Cultures of Human Hepatocytes Modulated by ToxCast Chemicals. J Toxicol Environ Health B Crit Rev. 2010 Feb;13(2-4):329-46.

Rotroff DM, Wetmore BA, Dix DJ, Ferguson SS, Clewell HJ. Incorporating Human Dosimetry and Exposure into High-Throughput In Vitro Toxicity Screening. Toxicol Sci. 2010 Oct;117(2):348-58.

 

Toxicology

 

LeCluyse EL, Witek RP, Andersen ME, Powers MJ. Organotypic liver culture models: Meeting current challenges in toxicity testing. Critical Reviews in Toxicology. 2012 Early online 1-48

Martinez SM, Bradford BU, Soldatow VY, Kosyk O, Sandot A. Evaluation of an In Vitro Toxicogenetic Mouse Model for Hepatotoxicity. Toxicol Appl Pharmacol. 2010 Dec 15;249(3):208-16.

 

Drug Metabolism

 

Sahi J, Grepper S, Smith C. Hepatocytes as a Tool in Drug Metabolism, Transport and Safety Evaluations in Drug Discovery. Curr Drug Discov Technol. 2010 Sep;7(3):188-98.

Hewitt NJ, Lechón MJ, Houston JB, Hallifax D, Brown HS. Primary Hepatocytes: Current Understanding of the Regulation of Metabolic Enzymes and Transporter Proteins, and Pharmaceutical Practice for the Use of Hepatocytes in Metabolism, Enzyme Induction, Transporter, Clearance, and Hepatotoxicity Studies. Drug Metab Rev. 2007;39(1):159-234.

 

Hepatocyte Cell Biology

 

LeCluyse EL, Alexandre E. Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue. Methods Mol Biol. 2010;640:57-82.

 

Co-cultures

 

Sunman JA, Hawke RL, LeCluyse EL, Kashuba AD. Kupffer Cell-Mediated IL-2 Suppression of CYP3A Activity in Human Hepatocytes. Drug Metab Dispos. 2004 Mar;32(3):359-63.

 

Posters

Posters

2015

Use of Cryopreserved Plateable Hepatocytes in Conjunction with Hepextend™

Optimization of Primary Hepatocyte Media for Evaluation of Drug Metabolism and Drug-Induced Liver Injury

Isolation and Characterization of Quiescent Human Hepatic Stellate Cells For Physiological Modeling of Liver Metabolism and Function

2014

Comparative Analysis of DILI Compounds on Cytokine Production in Kupffer Cells and Hepatocytes

A DIY Model for Generation of Robust 3D Epidermal Skin Equivalents Composed of Normal Human Primary Epidermal Keratinocytes

Reproducible Generation of 3D Corneal Models from Normal Human Limbal Derived Progenitor Cells

Long Term Hepatocyte Culture for Drug Safety Assessment Screening

In Vitro Modeling of Inflammatory Response Associated with Cholestatic Injury in HepaRG

2012

Optimization of a Plated Cryopreserved Human Hepatocyte Model for Assessment of the Metabolic Stability of Low Clearance Compounds

Enzyme Activity and Transporter Facilitated Uptake Comparisons: Multi-donor Pooled Cryopreserved Hepatocytes (HEP10™) to Single Donor Constituent Lots

Modeling of Cytokine-Induced Liver Hepatotoxicity of Trovafloxacin Using Co-cultures of Hepatocytes and Kupffer Cells

2011

Cryopreserved HepaRG Cells An Alternative In Vitro Screening Tool for Human Hepatic Drug Metabolism

Use of Cryopreserved Primary Rat and Human Kupffer Cells and Hepatocytes in Co-cultures to Study APAP Cytotoxicity

Long Term Storage Effects on the Metabolic Activity of Cryopreserved Primary Human and Rat Hepatocytes

2010

Development and Testing of Cryopreserved Carp Hepatocytes for the Estimation of Bioconcentration in Fish

In Vitro Metabolic Enzyme Activity Characterization in Cryopreserved, Freshly Isolated Hepatocytes and Liver S9 Fraction from Rainbow Trout

In Vitro Fish Metabolism and Metabolite Profiling of Several Fragrances using Rainbow Trout Liver S9 Fraction and Cryopreserved Hepatocytes

Taurocholate or Glycocholate – Which is the More Appropriate Substrate for Assessing Hepatic Bile Salt Transport?

Influence of Gender and Ethnicity on Drug Metabolizing Enzymes in Primary Human Hepatocytes

Utilization and Optimization of Cryopreserved Human Hepatocytes as a Model to Assess CYP450 Inhibition

Comparison of Metabolic Capacities of Fresh and Cryopreserved Human Hepatocytes Isolated from the Same Donor: Metabolic Stability, Plated Metabolism, Metabolite ID Applications

Induction of Transporter Expression and Modulation of Hepatobiliary Disposition using Sandwich Cultures of Human Hepatocytes

Genotyped, Cryopreserved Suspensions of Human Hepatocytes as a Tool for Investigating Drug Metabolism in Polymorphic Individuals

Suppression of OATP1B1, OATP1B3, and OATP2B1 Transporters in Primary Cryopreserved Human Hepatocytes Following Lipid Delivery of Stealth siRNA™

Time-Course Disposition of Ritonavir and Prototypical Hepatic Inducers in Cultures of Primary Human Hepatocytes: Context for Induction & Inhibition Concentration Responses

Publications

Publications

DDI Induction | Environmental Tox | Toxicology | Drug Metabolism |  Hepatocyte Cell Bio | Co-cultures

 

DDI Induction

 

Li Y, Zhou D, Ferguson SS, Dorff P, Simpson TR, Grimm SW. In Vitro Assessment of Metabolic Drug–Drug Interaction Potential of AZD2624, Neurokinin-3 Receptor Antagonist, Through Cytochrome P(450) Enzyme Identification, Inhibition, and Induction Studies. Xenobiotica. 2010 Nov;40(11):721-9.

Li L, Hassan HE, Tolson AH, Ferguson SS, Eddington ND, Wang H. Differential Activation of Pregnane X Receptor and Constitutive Androstane Receptor by Buprenorphine in Primary Human Hepatocytes and HepG2 Cells. J Pharmacol Exp Ther. 2010 Sep.

 

Rana R, Chen Y, Ferguson SS, Kissling GE, Surapureddi S. Hepatocyte Nuclear Factor 4alpha Regulates Rifampicin-Mediated Induction of CYP2C Genes in Primary Cultures of Human Hepatocytes. Drug Metab Dispos. 2010 Apr;38(4):591-9.

 

Lau AJ, Yang G, Rajaraman G, Baucom CC, Chang TK. Differential Effect of Meclizine on the Activity of Human Pregnane X Receptor and Constitutive Androstane Receptor. J Pharmacol Exp Ther. 2010 Dec.

Lau AJ, Yang G, Rajaraman G, Baucom CC, Chang TK. Human Pregnane X Receptor Agonism by Ginkgo Biloba Extract: Assessment of the Role of Individual Ginkgolides. J Pharmacol Exp Ther. 2010 Dec;335(3):771-80.

Graham RA, Tyler LO, Krol WL, Silver IS, Webster LO. Temporal Kinetics and Concentration-Response Relationships for Induction of CYP1A, CYP2B, and CYP3A in Primary Cultures of Beagle Dog Hepatocytes. J Biochem Mol Toxicol. 2006;20(2):69-78.

Faucette SR, Sueyoshi T, Smith CM, Negishi M, Lecluyse EL. Differential Regulation of Hepatic CYP2B6 and CYP3A4 Genes by Constitutive Androstane Receptor but Not Pregnane X Receptor. J Pharmacol Exp Ther. 2006 Jun;317(3):1200-9.

Ferguson SS, Chen Y, LeCluyse EL, Negishi M, Goldstein JA. Mol Pharmacol. Human CYP2C8 is Transcriptionally Regulated by the Nuclear Receptors Constitutive Androstane Receptor, Pregnane X Receptor, Glucocorticoid Receptor, and Hepatic Nuclear Factor 4alpha. 2005 Sept;68(3):747-57.

Smith CM, Graham RA, Krol WL, Silver IS, Negishi M. Differential UGT1A1 Induction by Chrysin in Primary Human Hepatocytes and HepG2 Cells. J Pharmacol Exp Ther. 2005 Dec;315(3):1256-64.

Madan A, Graham RA, Carroll KM, Mudra DR, Burton LA. Effects of Prototypical Microsomal Enzyme Inducers on Cytochrome P450 Expression in Cultured Human Hepatocytes. Drug Metab Dispos. 2003 Apr;31(4):421-31.

Wang H, LeCluyse EL. Role of Orphan Nuclear Receptors in the Regulation of Drug-Metabolising Enzymes. Clin Pharmacokinet. 2003;42(15):1331-57.

LeCluyse EL. Pregnane X Receptor: Molecular Basis for Species Differences in CYP3A Induction by Xenobiotics. Chem Biol Interact. 2001 May 16;134(3):283-9.

LeCluyse E, Madan A, Hamilton G, Carroll K, DeHaan R. Expression and Regulation of Cytochrome P450 Enzymes in Primary Cultures of Human Hepatocytes. J Biochem Mol Toxicol. 2000;14(4):177-88.

McCune JS, Hawke RL, LeCluyse EL, Gillenwater HH, Hamilton G. In Vivo and In Vitro Induction of Human Cytochrome P4503A4 by Dexamethasone. Clin Pharmacol Ther. 2000 Oct;68(4):356-66.

 

Environmental Tox

 

Budinsky RA, LeCluyse EL, Ferguson SS, Rowlands JC, Simon T. Human and Rat Primary Hepatocyte CYP1A1 and 1A2 Induction with 2,3,7,8-Tetrachlorodibenzo-p-dioxin, 2,3,7,8-Tetrachlorodibenzofuran, and 2,3,4,7,8-Pentachlorodibenzofuran. Toxicol Sci. 2010 Nov;118(1):224-35.

Rotroff D, Beam A, Freeman K, Farmer A, Bondell H. Xenobiotic Metabolizing Enzyme and Transporter Gene Expression in Primary Cultures of Human Hepatocytes Modulated by ToxCast Chemicals. J Toxicol Environ Health B Crit Rev. 2010 Feb;13(2-4):329-46.

Rotroff DM, Wetmore BA, Dix DJ, Ferguson SS, Clewell HJ. Incorporating Human Dosimetry and Exposure into High-Throughput In Vitro Toxicity Screening. Toxicol Sci. 2010 Oct;117(2):348-58.

 

Toxicology

 

LeCluyse EL, Witek RP, Andersen ME, Powers MJ. Organotypic liver culture models: Meeting current challenges in toxicity testing. Critical Reviews in Toxicology. 2012 Early online 1-48

Martinez SM, Bradford BU, Soldatow VY, Kosyk O, Sandot A. Evaluation of an In Vitro Toxicogenetic Mouse Model for Hepatotoxicity. Toxicol Appl Pharmacol. 2010 Dec 15;249(3):208-16.

 

Drug Metabolism

 

Sahi J, Grepper S, Smith C. Hepatocytes as a Tool in Drug Metabolism, Transport and Safety Evaluations in Drug Discovery. Curr Drug Discov Technol. 2010 Sep;7(3):188-98.

Hewitt NJ, Lechón MJ, Houston JB, Hallifax D, Brown HS. Primary Hepatocytes: Current Understanding of the Regulation of Metabolic Enzymes and Transporter Proteins, and Pharmaceutical Practice for the Use of Hepatocytes in Metabolism, Enzyme Induction, Transporter, Clearance, and Hepatotoxicity Studies. Drug Metab Rev. 2007;39(1):159-234.

 

Hepatocyte Cell Biology

 

LeCluyse EL, Alexandre E. Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue. Methods Mol Biol. 2010;640:57-82.

 

Co-cultures

 

Sunman JA, Hawke RL, LeCluyse EL, Kashuba AD. Kupffer Cell-Mediated IL-2 Suppression of CYP3A Activity in Human Hepatocytes. Drug Metab Dispos. 2004 Mar;32(3):359-63.

 

Brochures

Brochures

ADME/tox Product Brochure

Rat Kupffer Cryopreserved Co-cultures

HEP10 Pooled Human Cryopreserved Hepatocytes

Cryopreserved HepaRG™ Cells

For Research Use Only. Not for use in diagnostic procedures.