LanthaScreen TR-FRET Tools for Assay Development

Flexible tools for TR-FRET assay development:

• Choose from Europium or Terbium donor chelate labels 
  
• Obtain labeled anti-species, anti-epitope and streptavidin for your assay development project
  
• Comparable performance to other available TR-FRET tools
  
• Label your own molecules or let us do it for you
  

Whether you are routinely running assays in-house to support discovery programs, or need generic reagents to quickly build new TR-FRET assays, our portfolio of LanthaScreen reagents can enable your assay development program. Our LanthaScreen toolbox offers a collection of Terbium and Europium reagents for a true 'mix and match' experience.  Our Europium labeled antibodies are not only compatible with fluorophores from Life Technologies (such as Alexa Fluor 633 and 647), but are compatible with other red fluorophores such as CyDyes and ULight dyes.

Choose from:

• anti-Epitope Tag Antibodies
• Secondary antibodies
• Streptavidin and Biotin
  
• Reactive chelate labeling reagents and labeling services

Epitope tags facilitate the purification of proteins for use in many applications.  Such tags can also be used to indirectly label proteins through the use of LanthaScreen anti-epitope tag antibodies.  

Indirect labeling of a protein through the use of an anti-epitope tag antibody can be advantageous when working with partially purified protein, or in cases in which the direct labeling of the protein would negatively impact protein activity.  

Our products are available as kits or stand alone antibodies.  Trial kits contain 100mL of TR-FRET dilution buffer (PV3574), which is recommended for antibody dilution, and a positive control peptide for the antibody when available.  Europium antibodies are compatible with fluorophores purchased from Life Technologies or other suppliers including but not limited to Alexa Fluor 647, Cy3, Cy5, and ULight dyes. Terbium antibodies are compatible with fluorescein or GFP.

Our secondary anti-species antibodies bind to their corresponding species IgG with low nanomolar apparent Kds, and show negligible cross-reactivity with other species. These antibodies can be used to rapidly develop assays when an anti-target antibody is already available.  The labeled anti-species antibody will bind to the anti-target IgG completing the TR-FRET pair for easy assay development.

Our products are available as kits or stand alone antibodies.  Kits contain a positive control peptide for the antibody, and 100 mL of TR-FRET dilution buffer (PV3574) as a dilutent for the antibody during biochemical assay development.

Life Technologies offers do-it-yourself reagents or a custom service for labeling of your antibody or protein of interest.  Reagents include amine-reactive, isothiocyanate-functionalized LanthaScreen chelates and thiol-reactive, maleimide-functionalized LanthaScreen terbium chelates for development of TR-FRET assays in-house.  Europium chelate reagents are available on a custom basis.

Alternatively, we can custom label your antibody (or other protein) with either a europium or terbium chelate.  Let us know what antibody you would like us to label (vendor information) or supply antibody in a buffer without carrier protein (i.e. BSA), and we will label it for you.  
To learn more about custom labeling and gain access, submit an online inquiry