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DNA, RNA, and protein quantification for a single or multiple samples
Both Qubit instruments are accurate, sensitive, fast, and simple fluorometers that use the target-specific fluorescent dyes in Qubit Assays to quantify DNA, RNA, or proteins. Choose the model that aligns with typical usage in your lab. The Qubit 4 Fluorometer is designed for low-throughput processing, whereas the Qubit Flex Fluorometer is designed for mid-throughput processing, quantifying up to eight samples at a time, with the flexibility to process other increments. Qubit Flex Fluorometers also support add-on SAE software for 21 CFR Part 11 compliance support.
Which Qubit Fluorometer is best for you?
Both Qubit models work the same way and share common features:
- Qubit fluorescence quantification technology
- Simple and fast
- Onboard Reagent Calculator
- Fluorometer mode
- Accurate, specific, and sensitive
- Requires as little as 1 µL of sample
- Broad range of assays and applications
The key difference between the models is that the Qubit 4 Fluorometer quantifies one sample at a time, while the Qubit Flex Fluorometer quantifies up to eight samples at a time using Qubit Flex Assay Tube Strips. For more than eight samples, simply insert more tube strips.
Tip: For high-throughput quantification, consider using a microplate reader with fluorescence capabilities. The Thermo Scientific Varioskan ALF and Varioskan LUX Multimode Microplate Readers are compatible with Quant-iT assays to quantify the same analytes as Qubit Fluorometers and assays. For a comparison, see our RNA/DNA Quantification page.
Selection guide
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| Product summary | |
| Highly accurate, easy-to-use quantification of a single sample of DNA, RNA, or protein |
Highly accurate, easy-to-use quantification of a up to eight samples of DNA, RNA, or protein |
Detailed comparison table
Feature |
Qubit 4 Fluorometer |
Qubit Flex Fluorometer |
|---|---|---|
Sample throughput |
1 sample in 3 seconds |
1–8 samples in 3 seconds |
User interface |
5.7-inch color touchscreen |
8-inch color touchscreen |
Onboard calculators |
Reagent calculator |
Reagent calculator Molarity calculator Normalization calculator |
| System Check | Qubit 4 System Verification Assay Kit |
Qubit Flex System Verification Assay Kit |
Fluorometer mode |
Yes |
Yes |
Programmable open format |
Yes, MyQubit |
No |
Instrument footprint |
13.6 x 25 x 5.5 cm 5.4 x 10 x 2.2 in. |
18.6 x 28.2 x 10.3 cm 7.3 x 11.1 x 4.1 in. |
Sample data storage |
1,000 samples |
10,000 samples |
Data export |
|
|
Light sources |
Blue LED (peak ~470 nm) Red LED (peak ~635 nm) |
Blue LED (peak ~460–480 nm) Red LED (peak ~620–640 nm) |
Excitation filters |
Blue LED (430–495 nm) Red LED (600–645 nm) |
Blue LED (456–484 nm) Red LED (612–644 nm) |
Emission filters |
Green (510–580 nm) Red (665–720 nm) |
Green (513–563 nm) Far-red (671–693 nm) |
| 21 CFR Part 11 software support | Not available | Optional add-on SAE software |
Qubit 4 Fluorometer
The Invitrogen Qubit 4 Fluorometer accurately and quickly measures the concentration of DNA, RNA, or protein in a single sample. It can also be used to assess RNA integrity and quality. The easy-to-use touchscreen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.
Qubit Flex Fluorometer
The Invitrogen Qubit Flex Fluorometer accurately and quickly measures the concentration of DNA, RNA, or protein in up to eight samples simultaneously. Compared to single-sample microvolume fluorometers, using the Qubit Flex system for multiple samples can reduce your time to data by up to 50%. For 21 CFR Part 11 compliance support, Qubit Flex Fluorometers have an optional add-on SAE software available.
For Research Use Only. Not for use in diagnostic procedures.