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Cell Isolation, Expansion and Differentiation Information
Cell isolation, expansion and differentiation are critical processes and techniques for studying cell biology. Here you will find educational resources including videos, application notes and articles to help you better understand these processes.
Cell isolation, expansion and differentiation features
Application note
Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytes
This application note describes a flow cytometric method for the simultaneous quantification of Oct4, a canonical marker of pluripotency, and Nkx2.5, a marker of cardiac fate, in hPSCs induced to differentiate towards cardiomyocytes.
Technology video
The two worlds of cell separation
This video explains the differences between microparticle and nanoparticle cell isolation techniques.
Cell isolation, expansion and differentiation learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2017) | Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytes | Attune/Attune NxT Flow Cytometer, cell differentiation, flow cytometry, multicolor flow cytometry, stem cell research |
| BioProbes article (2015) | Isolation and characterization of exosomes using magnetic beads | antibodies, exosomes, flow cytometry, fluorescence, immunophenotyping, multiplex ELISA/Luminex, particles, protein blotting, western detection |
| Scientific poster (2010) | A comparison of three techniques to induce efficient ex vivo T cell expansion | Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometry, fluorescent dyes, immunology, magnetic beads, multicolor flow cytometry, viability |
| BioProbes article (2009) | A flow cytometry–based method for isolating neural stem cells | Alexa Fluor, cell isolation, flow cytometry, fluorescent dyes, neuroscience, stem cell research |
| BioProbes article (2009) | Isolate T cells directly from whole blood | cell isolation, cell proliferation, Click-iT, flow cytometry, fluorescent dyes, immunology, magnetic beads |
| BioProbes article (2009) | Isolation and expansion of mouse Treg cells using Dynabeads technology | Alexa Fluor, cell expansion, cell isolation, flow cytometry, fluorescent dyes, immunology, magnetic beads |
| Scientific poster (2009) | A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells | cell culture, cell expansion, flow cytometry, fluorescent dyes, gene expression, immunophenotyping, stem cell research |
| BioProbes article (2008) | Putting regulatory T cells to work—Isolate and expand CD4+ CD25+ Treg cells using Dynabeads magnetic separation technology | cell expansion, cell isolation, flow cytometry, fluorescent dyes, immunology, magnetic beads, reagents |
| BioProbes article (2008) | Single-step isolation of functional human NK cells | antibodies, cell isolation, flow cytometry, fluorescent dyes, immunology, magnetic beads, sample preparation |
| BioProbes article (2007) | The latest in stem cell research | antibodies, antibody labeling, cell differentiation, cell expansion, flow cytometry, fluorescent dyes, growth factors, fluorescence microscopy/fluorescence imaging, stem cell research |
| Technology video | The Two Worlds of Cell Separation | cell isolation, immunology |