Search Thermo Fisher Scientific
- Contact Us
- Quick Order
-
Don't have an account ? Create Account
Search Thermo Fisher Scientific
Bioactive proteins such as cytokines and growth factors interact with cells to modulate their behavior. They do this with a certain potency which may be expressed as an EC50, specific activity, or in international units (IU).
An international unit (IU) is a unit of activity for a biological substance that has been tested against an international standard or reference reagent. Potency (IU/ng) of a given biological reference standard is determined by an International Collaborative Study conducted by the World Health Organization (WHO).
Bioactive proteins that have been tested and assigned an internationally accepted unit of biological potency (i.e., IU/ng) are available as international standards or reference materials. Organizations such as the National Institute for Biological Standards and Control (NISBC) test, store, and distribute biological reference standards to allow laboratories across the world to compare their bioactive proteins to these standards.
Bioactive recombinant proteins can have different activities when produced by different labs and protocols, so they should be compared to a reference standard to determine relative potency. The use of IU is intended to simplify comparisons of biological activity of proteins or enzymes obtained from different sources.
Where possible, Thermo Fisher Scientific determines International Unit (IU) values for Gibco PeproTech recombinant proteins through multiple side-by-side comparisons of the PeproTech protein against the analogous WHO international standard or reference standard in a standard biological activity assay. Performing multiple comparison tests helps account for any outliers due to possible variations with the assay. These tests provide a reliable quantification of the PeproTech product’s activity in relation to the activity of the WHO international standard.
The IU values provided are applicable across all lot numbers of a specific catalog number, as testing covers multiple lots of bioactive protein with comparable activity. These values are based on comparison testing and are subject to change.
Cytokine name | Catalog number beginning with | Bioassay cell line | WHO catalog number | PeproTech protein activity (IU/ng) |
---|---|---|---|---|
Human Activin A | GMP120-14E | mouse MPC-11 | 91/626 (0.001 IU/ng) | 0.001 |
Human EGF | GMP100-15 | mouse Balb/c 3T3 | 91/530 (1.0 IU/ng) | 3.15 |
Human FGF-basic | GMP100-18B | mouse Balb/c 3T3 | 90/172 (0.8 IU/ng) | 2.1 |
Human Flt3-Ligand | GMP300-19 | human AML5 | 96/532 (1.0 IU/ng) | 1.0 |
Human IL-2 | GMP200-02 | mouse CTLL-2 | 86/500 (13.7 IU/ng) | 3.59 |
Human IL-3 | GMP200-03 | human TF-1 | 91/510 (1.7 IU/ng) | 1.42 |
Human IL-6 | GMP200-06 | mouse 7TD1 | 89/548 (100 IU/ng) | 115 |
Human IL-7 | GMP200-07 | mouse 2E8 | 90/530 (100 IU/ng) | 183 |
Human IL-15 | GMP200-15 | mouse CTLL-2 | 95/554 (10 IU/ng) | 34 |
Human SCF | GMP300-07 | human TF-1 | 91/682 (1.0 IU/ng) | 0.79 |
Human TPO | GMP300-18 | human MO7e | 03/124 (10 IU/ng) | 0.97 |
Cytokine name | Catalog number beginning with | Bioassay cell line | WHO catalog number | PeproTech protein activity (IU/ng) |
---|---|---|---|---|
Human IL-1α | 200-01A AF-200-01A | mouse D10S | 86/632 | 64.9 |
Human IL-1β | 200-01B AF-200-01B | mouse D10S | 86/680 | 70.3 |
Human IL-2 | 200-02 AF-200-02 | mouse CTLL-2 | 86/500 | 2.18 |
Human IL-3 | 200-03 | human TF-1 | 91/510 | 2.8 |
Human IL-4 | 200-04 | human TF-1 | 88/656 | 16.1 |
Human IL-6 | AF-200-06 | mouse 7TD1 | 89/548 | 123 |
Human IL-7 | 200-07 AF-200-07 | mouse 2E8 | 90/530 | 117 |
Human IL-10 | 200-10 AF-200-10 | mouse MC/9 | 93/722 | 22.6 |
Human IL-13 | 200-13 AF-200-13 | human TF-1 | 94/622 | 0.79 |
Human IL-15 | 200-15 AF-200-15 | mouse CTLL-2 | 95/554 | 28.7 |
H/M/R Activin A | 120-14 120-14P AF-120-14E | mouse MPC-11 | 91/626 | .001 |
Human BDNF | 450-02 AF-450-02 | rat C6 | 96/534 | 1.9 |
H/M/R BMP-2 | 120-02C | mouse ATDC-5 | 93/574 | 0.9 |
Human EGF | AF-100-15 | mouse Balb/c 3T3 | 91/530 | 1.0 |
Human EPO | 100-64 | human TF-1 | 11/170 | 0.16 |
Human FGF-basic | 100-18B AF-100-18B | mouse Balb/c 3T3 | 90/712 | 0.65 |
Human Flt3-Ligand | 300-19 AF-300-19 | human AML5 | 96/532 | 1.1 |
Human G-CSF | 300-23 AF-300-23 | mouse NFS-60 | 09/136 | 101.3 |
Human GDNF | 450-10 AF-450-10 | human SH-SY5Y | 09/266 | 0.78 |
Human GM-CSF | 300-03 AF-300-03 | human TF-1 | 88/646 | 12.8 |
Human HGF | 100-39 | monkey 4MBr-5 | 96/564 | 0.21 |
Human IFN-β | 300-02BC | human TF-1 | 00/572 | 504 |
Human IFN-γ | 300-02 AF-300-02 | human HT-29 | 87/586 | 1.94 |
Human IGF-I | 100-11 AF-100-11 | mouse FDC-P1 | 91/554 | .001 |
Human IGF-II | 100-12 AF-100-12 | mouse FDC-P1 | 96/538 | 0.6 |
Human KGF | 100-19 AF-100-19 | monkey 4MBr-5 | 03/150 | 2.33 |
Human LIF | 300-05 AF-300-05 | human TF-1 | 93/562 | 12.2 |
Human M-CSF | 300-25 AF-300-25 | mouse M-NSF-60 | 89/512 | 100 |
Human β-NGF | 450-01 | human TF-1 | 93/556 | 0.53 |
Human PDGF-BB | 100-14B AF-100-14B | mouse Balb/c 3T3 | 94/728 | 2.65 |
Human SCF | 300-07 | human TF-1 | 91/682 | 1.7 |
Human TGF-β1 | 100-21 100-21C AF-100-21C | mouse HT-2 | 89/514 | 9 |
Human TGF-β3 | 100-36E AF-100-36E | mouse HT-2 | 09/234 | 5.64 |
Human TNF-α | 300-01A | mouse L929 | 12/154 | 39.1 |
Human sTRAIL/Apo2L | 310-04 | human LN-18 | 04/166 | 1.45 |
Human VEGF165 | 100-20 AF-100-20 | HUVEC | 02/286 | 0.69 |
Murine IL-2 | 212-12 AF-212-12 | mouse CTLL-2 | 93/566 | 129 |
Murine IL-3 | 213-13 AF-213-13 | mouse NFS-60 | 91/662 | 132 |
Murine IL-4 | 214-14 AF-214-14 | mouse HT-2 | 91/656 | 19.8 |
Murine IL-6 | 216-16 AF-216-16 | mouse 7TD1 | 93/730 | 322 |
Murine GM-CSF | 315-03 AF-315-03 | mouse FDC-P1 | 91/658 | 44.3 |
Learn what recombinant proteins are, what they are used for, and what types are available
Learn about the PeproTech brand of recombinant proteins
Learn about all aspects of cell culture
See which recombinant proteins are used to generate organoids
Procedures and buffers for performing western blots
Explore the role of proteins and cytokines in various signaling pathways
Find Getting Started guides, protocols, troubleshooting, and other resources
Search the database to find answers to your questions
Get technical or customer support for any recombinant protein; find SDS, CoA, and phone numbers based on a specific product
Get in touch with GMP technical and regulatory experts
Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.