What is a plasmid maxiprep?

A plasmid maxiprep is a laboratory technique used to isolate and purify a large amount of plasmid DNA from bacterial cultures on a large scale. It is a step up from the smaller scale miniprep and midiprep methods. The maxiprep procedure typically involves similar steps to the midiprep, such as bacterial cell lysis, removal of cellular debris, and purification of the plasmid DNA. However, the maxiprep yields a significantly higher quantity of plasmid DNA, usually in the range of several hundred micrograms to milligrams, making it suitable for applications that require a large amount of pure plasmid DNA, such as large-scale cloning, protein production, or viral vector production.


When to use a plasmid DNA maxi kit?

A maxiprep scale kit should be used when attempting to purify plasmid from an overnight bacterial culture with a volume between 100–500 mL. When choosing a plasmid maxiprep kit, scientists should prioritize high-quality plasmid DNA that is pure, intact, and free from contaminants. This is crucial for helping ensure reliable and reproducible results in downstream applications, helping instill confidence in the experimental outcomes.

The Thermo Fisher Scientific GeneJET Plasmid Maxiprep Kit allows for efficient isolation of high-quality plasmid DNA from bacterial cultures, recovering up to 750 μg per prep. With a fast 60-minute procedure and a convenient spin column format, this kit offers high yields and concentrations, making it suitable for various molecular biology procedures.

The PureLink HiPure Plasmid Maxiprep Kit isolates transfection-grade plasmid DNA from E. coli, yielding 500–850 μg of highly pure DNA. With no extra steps required to remove contaminants, this kit can help save time and labor. It is versatile, suitable for purifying various types and sizes of plasmid DNA, including BAC, bacmids, and ssM13 DNAs.

Use the table below to help you choose the right solution for your plasmid DNA maxiprep.


Which plasmid DNA maxiprep kit is right for you?

 Order nowOrder nowOrder nowOrder now
 Quick and cost-effectiveMost widely accepted protocol for transfectionRapid purification with integrated lysate clarification filterRapid purification with integrated lysate clarification filter and precipitator
 GeneJET Plasmid Maxiprep Kit PureLink HiPure Plasmid Maxiprep Kit PureLink HiPure Plasmid Filter Maxiprep Kit PureLink HiPure FP (Filter and Precipitator) Maxiprep Kit
Purity gradeMolecularTransfectionTransfectionTransfection
Total protocol time60 min120 min90 min60 min
YieldUp to 750 µgUp to 1 mgUp to 1 mgUp to 1 mg
TechnologySilica membraneAnion-exchange gravity-flow columnAnion-exchange gravity-flow columnAnion-exchange gravity-flow column, precipitator
Clarification filterNoNoYes, save 30 minutesYes, save 30 minutes
Prep size10, 15 preps10, 15 preps10, 15 preps10, 25 preps

Explore: Plasmid DNA Miniprep
Explore: Plasmid DNA Midiprep

Video: How to purify transfection grade plasmid DNA

Learn how to purify transfection grade plasmid DNA with the Invitrogen PureLink HiPure Plasmid Filter Maxiprep Kit.

Resources
  • Protocol Videos—Videos to help you isolate nucleic acids using a variety of techniques.
  • DNA & RNA Selection Guide—Find the right purification products.
  • Videos—View our library of DNA & RNA purification and analysis videos.
  • Application Notes—Explore our application notes from scientists sharing data for isolation products
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