Custom DNA Oligos | Thermo Fisher Scientific

Oligonucleotides are the starting point for many of today’s biology research, drug discovery, and diagnostics applications. These highly technical applications demand high-quality oligonucleotides for success. Our oligos are made to your specifications with rigorous quality control and quick turnaround for use in a variety of applications, including PCR, cloning, sequencing, and gene detection.

Explore the easy-to use ordering portals for your tube and plate oligos, our complex and large-scale oligo services, and additional resources below.

Available custom oligo formats

	Standard oligos in tubes	Standard oligos in plates	Complex and large-scale oligos
	Order now	Order now	Special requests
Scales	25 nmol, 50 nmol, 200 nmol, 1 µmol, 10 µmol		Milligram to >15 gram final yield
Lengths	Up to 100 bases
Purification	Desalted, cartridge, HPLC, PAGE		Desalted, HPLC, RP-HPLC, IEX-HPLC, Dual-HPLC
Modifications	5’, internal, 3’
Formats	Tube Dried oligo shipped ambient Oligo resuspended in water shipped ambient or frozen	96- or 384-well plate Dried oligo shipped ambient Oligo resuspended in TE, Tris, or water shipped ambient or frozen	Custom formulations and packaging solutions
Ordering	Enter individually or bulk upload up to 200 sequences at once	Upload each plate separately using our template	Submit a request to our dedicated technical support team Commercial supply available
Shipping	Standard or next-day delivery, with free overnight shipping*	Standard delivery, with free overnight shipping*
Additional resources	Modification and configuration options Online ordering guide	Download 96-well template Download 384-well template	Complex and large-scale capabilities

All oligos are manufactured using controlled procedures certified against ISO 9001:2015 and ISO 13485:2016 standards.

Our oligos are made to your specifications, with rigorous quality control, and quick turnaround for use in a variety of applications, including PCR, cloning, sequencing, and gene detection.

Plate oligos

Now with free shipping*
One complete order with the same purification, synthesis scale, ship medium, and buffer.
96- or 384-well plate format
Standard delivery

Custom DNA oligos brochure

For a comprehensive guide on the small- and large-scale custom solutions and end-to-end services designed with your unique application in mind, access the Custom DNA oligos brochure.

Download brochure

Our custom oligonucleotide synthesis process

Our custom DNA oligos are synthesized on a highly automated, computer-controlled system using standard cyano-ethyl phosphoramidite chemistry. Post-synthesis QC employs electrospray ionization mass spectrometry (ESI MS) for all oligos. As a standard practice, 100% of synthesized oligos are measured for quality control whether provided in tubes or in plates.

Choose DNA oligos for your application

Which kind of oligos are right for me?

While our value oligo format represents our most economical and fastest way to order (25 or 50 nmol in tube formats with a range of purification options to suit your applications), if your research demands a scale larger than 50 nmol, a different format, or requires 5′, 3′, or internal modifications, then our standard DNA oligo formats have you covered (Table 1).

Table 1. Which kind of oligos are right for me?

Value oligos	Standard oligos
Price is calculated per oligo	Price is calculated per base
Length: 5–40 bases Scale: 25–50 nmol	Length: 5–100 bases Scale: from 25 nmol to 10 µmol
Not available with modifications	5′, internal, and 3′ modifications are available
Order now	Order now

Choosing the synthesis scale and purification option that is well suited for your application depends on the nature of your downstream applications. We can help you choose the right oligos and purification methods for your application (Table 2).

Table 2. Recommended oligo processing options by application

Application	Desalted	Cartridge	HPLC	PAGE
	25 nmole–10 µmole 5–100 bases Removes salts but not truncated sequences	50 nmole–1 µmole 7–55 bases Removes truncated sequences Provides 80% full-length sequence	50 nmole–10 µmole 10–55 bases Removes truncated sequences Provides >85% full-length sequence	50 nmole–10 µmole 7–100 bases Removes truncated sequences Provides > 90% full-length sequence
Standard PCR
Specialty PCR
Cloning
cDNA library construction
Sequencing
Fluorescent sequencing
Next generation sequencing
Mutagenesis
Antisense
Gel shift assays
Microarrays

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Pre-designed oligo(dT) & random DNA primers

Looking for pre-defined oligo sequences?

Pre-designed oligo(dT) & random DNA primers

In addition to our custom synthesis options for oligos, primers, probes, and genes, we offer random primers, oligo (dT) primers, 5’ labeled primers, gene specific primers for sequencing, forward and reverse sequencing primers and kits.

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Watch now: Learn how easy it is to order custom oligos online.

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* This promotion is open to customers in the US (excluding Puerto Rico) and Canada. Discount will apply to qualifying orders received by Life Technologies no later than December 31, 2024. Discount applies to list price in effect at the time order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

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