Detect Proteins in Western Blot

Find comprehensive detection solutions designed for your western blotting success

Quick visualization, precise quantitation, single-probe detection or multiplexing — we offer a range of reagents, kits and instruments for western blot detection and analysis.

Probing a western blot prior to data visualization involves a series of manual steps, many of which are individually short but collectively require significant hands-on time. We offer instruments to automate some of these tasks, significantly decreasing hands-on time.

Manual and automated procedures share three essential steps: blocking the membrane, probing with primary and secondary antibodies, and washing the membrane. The manual western blot processing workflow requires more than 10 hands-on steps and at least 4 hours before final detection can occur. Automated western blot processing using the Invitrogen Bandmate Automated Western Blot Processor or Invitrogen iBind or iBind Flex Western Device requires only a 15–30 minute setup time and no additional hands-on steps before you can move to final detection.