ExpiSf Baculovirus Expression System

Experience the first-ever chemically defined baculovirus expression system that can generate three times more protein compared to existing insect expression platforms. The ExpiSf Expression System enables greater consistency across multiple expression runs, all in less time. Put the newest member of the Expi system family to the test in your lab, from discovery to production.

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“The new ExpiSf Expression System offers a convenient virus generation and protein expression cell line in only two steps, for both secreted and intracellular proteins,we predict that using the ExpiSf system will save us about 30 percent in the time taken for protein expression and make cell maintenance simpler.”  Xuan Droz, Ph.D., project leader, Redbiotec AG

Undefined insect cell media components like yeastolate make it very difficult to get consistent performance. The Gibco ExpiSf system is the first ever baculovirus expression system that includes both a chemically defined medium and protein expression enhancer for reliable results you can count on. This insect cell expression system also utilizes a non-engineered derivative of Sf9 insect cells that have been adapted for high-density suspension growth and a specialized vector for insect cells.

Included in the ExpiSf Expression System:

1. Two vials of frozen ExpiSf9 Cells
2. 1 L ExpiSf CD Medium
3. One Bac-to-Bac Vector Kit*
4. 4 mL ExpiSf Enhancer
5. 0.5 mL MAX Efficiency DH10Bac Competent Cells*
6. 1 mL ExpiFectamine Sf Transfection Reagent
7. 500 mL Opti-MEM I Medium, with phenol red*
8. 24 Nalgene Single-Use PETG vented 125-mL Erlenmeyer Flasks**
   

*not included in China
** not included in all regions
 

The ExpiSf Expression System is designed to deliver consistent cell growth and protein expression, run after run. With our new Sf9 expression system, inconsistent results are a thing of the past. The superior performance of the chemically defined ExpiSf system delivers consistent results across expression runs and over multiple cell passages. One reliable system gives you one less variable to worry about.

It’s revolutionary—the ExpiSf system outperforms existing Sf9 expression platforms with 3x higher protein yield. The ExpiSf Expression System achieves protein yields that are vastly superior to traditional insect platforms. In performance tests against other suppliers’ media, the ExpiSf system demonstrated greater protein expression, delivering 3x more protein.

Figure 1. Recombinant protein titers in ExpiSf and other baculovirus expression systems. Expression levels of green fluorescent protein (GFP), Fc fusion protein, and tumor necrosis factor alpha (TNF-a) in ExpiSf and other baculovirus-based insect cell protein expression products are shown. ExpiSf protein titers are >3, >4, or >5 times those of other supplier’s insect products, respectively.

Figure 2. Expression of three different protein categories achieve expression levels >3x higher in the ExpiSf Expression System. Comparative western blotting analysis of a membrane protein (top panel), a receptor-type protein kinase (middle panel) and a tyrosine-protein kinase (bottom panel) produced in ExpiSf and control (Sf9 cells in Sf-900 II SFM) is shown. P0, P1, and P2 signify non-amplified (P0) or amplified (P1, P2) baculovirus stock used for the respective groups. *1.25x104 total cells/lane, **2.5x104 total cells/lane.
 

Table 1. ExpiSf is cost effective compared to other products for baculovirus-based insect protein expression systems.

System components work synergistically for maximum performance. Other suppliers offer separate reagents for protein production using the baculovirus expression vector system, but there is only one system that’s fully optimized with a streamlined workflow. The ExpiSf system contains integrated components that work together to deliver maximum protein yields.

The ExpiSf Expression System delivers protein in half the time. Bacmid DNA transfection in suspension using the ExpiSf system allows for efficient baculovirus generation without the need for virus amplification. That means you can get your protein in as few as six days.