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We’ve collected these protocols, publications, and scientific posters citing the use of Gibco KnockOut products for stem cell culture. A keyword search is available for the items listed in the table below. Alternatively, the columns may be sorted by resource type, resource name, application, or the products used. Our Stem Cell Research Learning Center is home to many more learning resources to help you maximize your stem cell research efforts and outcomes.
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| Type | Name | Application | Products used |
|---|---|---|---|
| Protocol | Creation of embryoid bodies from iPSCs using complete KnockOut Serum Replacement feeder-free medium | EB formation in human PSCs | KnockOut Serum Replacement; KnockOut DMEM/F-12 |
| Protocol | Culturing human embryonic stem cells (hESCs) on MEF-conditioned medium | Conditioned medium culture of human ESCs | KnockOut Serum Replacement; Geltrex hESC-Qualified Matrix; CELLstart CTS substrate |
| Protocol | Culturing induced pluripotent stem cells (iPSCs) on MEF-conditioned medium | Conditioned medium culture of human iPSCs | KnockOut Serum Replacement |
| Protocol | Derivation of dopaminergic neurons (from human embryonic stem cells) | Differentiation of human ESCs | KnockOut Serum Replacement; Geltrex hESC-Qualified Matrix; CELLstart CTS substrate |
| Protocol | Feeder-dependent culture of human embryonic stem cells (hESCs) | Feeder-dependent culture of human ESCs | KnockOut Serum Replacement |
| Protocol | Feeder-dependent culture of human induced pluripotent stem cells (hiPSCs) | Feeder-dependent culture of human iPSCs | KnockOut Serum Replacement |
| Protocol | Feeder-dependent culture and passaging mouse embryonic stem cells (mESCs) in KnockOut Serum Replacement with leukemia inhibitory factor (LIF) | Feeder-dependent culture of mouse ESCs | KnockOut Serum Replacement; KnockOut DMEM |
| Protocol | Formation of embryoid bodies (EBs) from mouse embryonic stem cells (mESCs) | EB formation in mouse ESCs | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2000) | Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture | Culture of human ESCs; comparison of FBS and KnockOut SR | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2001) | Feeder-free growth of undifferentiated human embryonic stem cells | Feeder-free culture of human hESCs | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2003) | Generating CNS neurons from embryonic, fetal, and adult stem cells | Differentiation of human SCs | KnockOut DMEM |
| Publication (2004) | Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor | Culture of human and mouse ESCs | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2004) | Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor | Culture of human ESCS; comparison of FBS and KnockOut SR | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2004) | Feeder layer- and serum-free culture of human embryonic stem cells | Feeder-dependent culture of hESCs | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2004) | Electromechanical integration of cardiomyocytes derived from human embryonic stem cells | Integration of human ESCs | KnockOut DMEM |
| Publication (2004) | Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media | Serum-free culture | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2005) | Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells | Feeder-dependent culture of hESCs; comparison of FBS and KnockOut SR | KnockOut Serum Replacement |
| Publication (2006) | An efficient method for the derivation of mouse embryonic stem cells | Derivation of mouse ESCs; comparison of FBS and KnockOut SR | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2006) | Common culture conditions for maintenance and cardiomyocyte differentiation of the human embryonic stem cell lines, BG01 and HUES-7 | Differentiation of human ESCs; comparison of FBS and KnockOut SR | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2006) | Unique gene expression signature by human embryonic stem cells cultured under serum-free conditions correlates with their enhanced and prolonged growth in an undifferentiated stage | Serum-free culture; comparison of FBS and KnockOut SR | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2006) | Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines | Serum-free culture; comparison of FBS and KnockOut SR | KnockOut Serum Replacement |
| Publication (2007) | New cell lines from mouse epiblast share defining features with human embryonic stem cells | Derivation of mouse ESCs | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2007) | Induction of pluripotent stem cells from adult human fibroblasts by defined factors | Generation of human iPSCs | KnockOut Serum Replacement |
| Publication (2007) | Generation of induced pluripotent stem cells in the absence of drug selection | Generation of mouse iPSCs; comparison of FBS and KnockOut SR | KnockOut Serum Replacement |
| Publication (2008) | Disease-specific induced pluripotent stem cells | Generation of human iPSCs | KnockOut Serum Replacement |
| Publication (2008) | Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2 | Generation of human iPSCs | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2008) | Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts | Generation of Rhesus monkey IPSCs | KnockOut Serum Replacement |
| Publication (2008) | Reprogramming of human somatic cells to pluripotency with defined factors | Reprogramming of human somatic cells | KnockOut DMEM |
| Publication (2008) | Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds | Reprogramming of mouse fibroblasts | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2009) | Derivation of induced pluripotent stem cells from pig somatic cells | Generation of pig iPSCs | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2010) | Characterization of human Huntington's Disease cell model from induced pluripotent stem cells | Characterization of human iPSCs | KnockOut Serum Replacement; KnockOut DMEM/F-12 |
| Publication (2010) | Human dermal stem cells differentiate into functional epidermal melanocytes | Differentiation of human SCs | KnockOut Serum Replacement; KnockOut DMEM/F-12 |
| Publication (2010) | Generation of novel rat and human pluripotent stem cells by reprogramming and chemical approaches | Generation of rat and human PSCs | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2010) | Efficient and rapid generation of induced pluripotent stem cells using an alternative culture medium | Generation of mouse iPSCs; comparison of FBS and KnockOut SR | KnockOut Serum Replacement |
| Publication (2011) | Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells | Differentiation of mouse ESCs | KnockOut DMEM/F-12 |
| Publication (2011) | Induced pluripotent stem cells for neural tissue engineering | Generation of human iPSCs | KnockOut DMEM/F-12 |
| Publication (2011) | Generation of germline-competent rat induced pluripotent stem cells. | Generation of rat iPSCs | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2011) | Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors | Reprogramming of fibroblasts; comparison of FBS and KnockOut SR | KnockOut Serum Replacement; KnockOut DMEM; KnockOut DMEM/F-12 |
| Publication (2012) | Optimization of protocols for derivation of mouse embryonic stem cell lines from refractory strains, including the non obese diabetic mouse | Derivation of mouse ESCs; comparison of FBS and KnockOut SR | KnockOut Serum Replacement; KnockOut DMEM |
| Publication (2012) | Signaling defects in iPSC-derived fragile X premutation neurons | Generation of human iPSCs | KnockOut Serum Replacement; KnockOut DMEM/F-12 |
| Publication (2012) | Efficient iPS cell production with the MyoD transactivation domain in serum-free culture | Serum-free culture; comparison of FBS and KnockOut SR | KnockOut Serum Replacement |
| Publication (2013) | Improved cell therapy protocols for Parkinson's disease based on differentiation efficiency and safety of hESC-, hiPSC-, and non-human primate iPSC-derived dopaminergic neurons | Differentiation of hESC, hiPSC and iPSCs | KnockOut Serum Replacement |
| Publication (2016) | Serum-based culture conditions provoke gene expression variability in mouse embryonic stem cells as revealed by single-cell analysis | Generation of mouse ESCs; comparison of FBS and KnockOut SR | KnockOut Serum Replacement |
| Scientific poster | Genome editing of α-Synuclein in iPSCs from a donor with multiple system atrophy | Genome editing of iPSCs grown on MEFs | KnockOut Serum Replacement; KnockOut DMEM |
| Scientific poster | CytoTune-iPS 2.0 Sendai Reprogramming Kit for high efficiency reprogramming of fibroblasts and blood-derived cells | Reprogramming of fibroblasts | KnockOut Serum Replacement; CytoTune 2.0 Reprogramming Kit |
| Scientific poster | Enabling successful reprogramming of peripheral blood mononuclear cells to induced pluripotent stem cells with the CytoTune-iPS 2.0 Sendai Reprogramming Kit | Reprogramming of PBMCs | KnockOut Serum Replacement; CytoTune 2.0 Reprogramming Kit |
| Scientific poster (ISSCR 2011) | Synthetic surfaces for the culture of human embryonic and adult stem cells | Matrices for growing hESCs and iPSCs | KnockOut Serum Replacement; Geltrex hESC-Qualified Matrix; CELLstart CTS |
| Scientific poster (ISSCR 2011) | High-quality episomal human iPSC derived from cord blood with a zero footprint: applications in serum-free and xeno-free culture | Serum-free culture | KnockOut Serum Replacement; Gibco Episomal hiPSCs |
| Scientific poster (ISSCR 2011) | Xeno-free Culture Systems for Stem Cells | Xeno-free culture of H9 hESCs | KnockOut DMEM; KnockOut SR XenoFree CTS; KnockOut SR GF Cocktail CTS |
| Scientific poster (ISSCR 2011) | Generation of zero-footprint iPSCs in xeno-free or feeder-free with CytoTune -iPS Sendai Reprogramming Kit | Xeno-free generation of iPSCs | KnockOut DMEM; KnockOut SR XenoFree CTS; KnockOut SR GF Cocktail CTS; CytoTune-iPS Sendai Reprogramming Kit |
| Scientific poster (ISSCR 2012) | Xeno-free culture systems for pluripotent stem cells | Generation of iPSCs | KnockOut DMEM CTS; KnockOut SR XenoFree CTS; KnockOut SR GF Cocktail CTS |
For Research Use Only. Not for use in diagnostic procedures.