Transfected cells may be analyzed for changes in genotypes and phenotypes, using nucleic acids, proteins, or functional assays.  We offer products optimized for performance and efficiency using each method.

Nucleic acid analysis

Select genomic DNA or RNA/cDNA

Genomic DNA isolation

PureLink Genomic DNA Mini Kit

PureLink Genomic DNA Mini Kit
  • Spin column-based isolation of genomic DNA from cells
  • 15 min preparation time
  • Up to 10 μg of purified DNA

Proofreading PCR

Platinum SuperFi II PCR Master Mix

Box and tubes of Platinum SuperFi II PCR Master Mix
  • Proofreading enzyme with exceptional PCR sequence accuracy
  • No need for annealing temperature calculation (works with a universal annealing temperature)
  • Amplification up to 40 kb with 30 sec/kb extension

Nonproofreading  PCR

Platinum II PCR Master Mix

Box and tubes of Platinum II PCR Master Mix
  • Fast PCR enzyme with 15 sec/kb extension
  • Enhanced PCR specificity, sensitivity, and yields
  • No need for annealing temperature calculation (works with a universal annealing temperature)

RNA isolation

PureLink RNA Mini Kit

PureLink RNA Mini Kit
  • Spin column-based isolation of total RNA from cells
  • 20 min preparation time
  • Up to 1,000 μg of purified RNA

Reverse transcription

SuperScript IV VILO Master Mix

Box and tubes of SuperScript IV VILO Master Mix
  • Reverse transcription in 10 min
  • Improved reproducibility due to single-tube master mix format
  • Higher cDNA yield (Ct values 2 cycles earlier on average) compared to other reverse transcription reagents

Proofreading PCR

Platinum SuperFi II PCR Master Mix

Box and tubes of Platinum SuperFi II PCR Master Mix
  • Proofreading enzyme with exceptional PCR sequence accuracy
  • No need for annealing temperature calculation (works with a universal annealing temperature)
  • Amplification up to 40 kb with 30 sec/kb extension

Nonproofreading PCR

Platinum II PCR Master Mix

Box and tubes of Platinum II PCR Master Mix
  • Fast PCR enzyme with 15 sec/kb extension
  • Enhanced PCR specificity, sensitivity, and yields
  • No need for annealing temperature calculation (works with a universal annealing temperature)

Looking for qPCR reagents?

 

Quantitative PCR (qPCR) or real-time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. 

Protein analysis

Protein sample preparation

 

Obtaining high-quality protein samples for analysis requires careful and precise protein extraction from cellular materials. Whether you’re using mechanical or detergent-based extraction workflows, our products are designed to help maintain the integrity of the proteins by preventing degradation and destabilization during this disruptive process. 

Protein quantitation

Pierce Rapid Gold BCA Protein Assay Kit
Pierce Rapid Gold BCA Protein Assay Kit
  • Assay time—5-min, room temperature reaction
  • Assay range—linear working range of 20 to 2,000 µg/mL

Protein detection

 

Find the right set of products that can assist you with detection and analysis of your protein. We offer a comprehensive suite of solutions for every step of the western blotting and ELISA workflows in order to help you obtain high-quality, publishable results with minimal time and effort.

Fluorescence imaging

 

We offer high quality fluorescence microscopy reagents and assays including cell structure and function dyes and stains, antifades and mountants, instrument calibration and set-up tools.  

Cell analysis

We offer a broad range of cell analysis research solutions including Gibco cell culture media and reagents, Invitrogen labeling and detection technologies, antibodies and immunoassays, Invitrogen EVOS cell imaging systems, Invitrogen Countess 3/3FL Automated Cell Counters, and the Invitrogen Attune flow cytometers.

Need more information?

Whether this is your first transfection or you’re a seasoned pro, we offer extensive protocols and support tools to help you achieve successful transfection experiments.  Review our transfection protocols and support pages to get the information you need, request samples and more.  

For Research Use Only. Not for use in diagnostic procedures.