• MORE TaqMan MicroRNA Assays
  • MORE Ambion Anti-miR™ miRNA Inhibitors
  • MORE Ambion Pre-miR™ miRNA Precursor

Sanger Institute miRBase, Release 10.1

The Sanger Institute miRBase Sequence Database is a sequence data repository that includes precursor and mature miRNA sequences for miRNAs whose expression has been confirmed and published. In a continuing effort to provide researchers with powerful tools for miRNA studies, Applied Biosystems offers new TaqMan MicroRNA Assays, and through Ambion, Anti-miR™ miRNA Inhibitors, and Pre-miR™ miRNA Precursors, to match the updated content of miRBase Sequence Database, Release 10.1.

Detect and Quantitate miRNA

TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan probes. These assays target only mature miRNAs, not their precursors, ensuring biologically relevant results.

miRNA Functional Studies

Ambion Pre-miR miRNA Precursor Libraries and Anti-miR miRNA Inhibitor Libraries address the needs of researchers seeking to upregulate and/or downregulate the levels of all known miRNAs to study endogenous or reporter gene regulation and phenotypic response. These collections of miRNA mimics and inhibitors enable rapid study of miRNA function and can be used to simplify identification of miRNAs that interact with a particular target transcript. Specific experimental designs include:

  • miRNA target site identification and validation
  • Screening for miRNAs that regulate the expression of a gene
  • Screening for miRNAs that affect a cellular process

Ambion Pre-miR miRNA Precursor Library Configurations

Pre-miR miRNA Precursor Library-Human V3:
470 miRNA mimics corresponding to 470 human mature miRNAs in the miRBase Sequence Database Release 9.2.

Pre-miR miRNA Precursor Library-Human V2:
(available while supplies last) 327 miRNA mimics corresponding to 327 miRNAs in miRBase Sequence Database Release 8.0.

Pre-miR miRNA Precursor Library–Mouse V3:
379 miRNA mimics corresponding to 379 mouse mature miRNAs in the miRBase Sequence Database Release 9.2.

Ambion Anti-miR miRNA Inhibitor Library Configurations

Anti-miR miRNA Inhibitor Library-Human V3:

470 miRNA inhibitors corresponding to 470 human mature miRNAs cataloged in the miRBase Sequence Database Release 9.2.

Anti-miR miRNA Inhibitor Library-Human V2:

(available while supplies last) 327 miRNA inhibitors corresponding to 327 miRNAs in miRBase Sequence Database Release 8.0.

Anti-miR miRNA Inhibitor Library–Mouse V3:

379 miRNA inhibitors corresponding to 379 mouse mature miRNAs in the miRBase Sequence Database Release 9.2.

Custom Ambion Pre-miR miRNA Precursor Libraries or Anti-miR miRNA Inhibitor Libraries: 50 or more Pre-miR miRNA Precursors or Anti-miR miRNA Inhibitors of your choice in the amount you specify.