Search Thermo Fisher Scientific
- Contact Us
- Quick Order
-
Don't have an account ? Create Account
Search Thermo Fisher Scientific
NucGreen Dead 488 ReadyProbes Reagent emits bright green fluorescence when bound to DNA and is suitable for staining nuclei in fixed-cell preparations and tissue sections. NucGreen Dead 488 reagent may also be used to measure viability and to measure cytotoxicity in kinetic live-cell assays.
First, fix and permeabilize cultured cells with a protocol appropriate for your sample.
1. Wash the cells 1–3 times in PBS as needed. |
2. Add two drops of NucGreen Dead 488 Reagent per milliliter of medium. |
3. Incubate for 5–15 minutes, protected from light. |
4. Remove the stain solution. |
5. Wash the cells 2–3 times in PBS. |
6. Image the cells. |
NucGreen Dead 488 | |
---|---|
Excitation/Emission (nm) | 504/523 |
Standard filter set | FITC or GFP |
EVOS Light Cube | FITC |
Storage conditions | Room temperature |
HeLa cells stained with NucGreen Dead 488 ReadyProbes reagent, indicating loss of plasma membrane integrity.