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The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line and an optimized medium and transfection kit that synergistically act to provide titers as much as 160x higher than the Gibco FreeStyle MAX CHO Expression System and 4x higher than the Gibco Expi293 Expression System. The ultrahigh yields of the ExpiCHO Expression System (up to 1–3 g/L for some proteins) allow you to scale down your expression run and achieve significant cost savings compared to other transient expression technologies.
Here we present protocols to scale down the system to:
Figure 1. Comparison of human IgG expression using a shaker flask or 96DWB.
Figure 2. Comparison of human IgG expression using a shaker flask or 24DWB.
Figure 3. Comparison of human IgG expression using a shaker flask or Nunc 50 mL Bioreactor Tube with different transfection volumes.
For Research Use Only. Not for use in diagnostic procedures.