Multiplex NGS based genotyping

Our portfolio of One Lambda AllType assays offers a complete next generation sequencing solution that provides single tube amplification for all eleven Class I and Class II loci, completely removing the need for amplicon pooling. Samples are transferred directly to library preparation and made ready for sequencing in a single workday. Sequencing analysis and individual allele assignments are automatically generated by our One Lambda TypeStream Visual NGS Analysis Software. Results are delivered in as little as three days.

 

Multiplex PCR for genotyping with NGS offers a simple, fast, and better workflow

Many labs might find NGS difficult to adopt due to the increased complexity, dedicated labor requirements and data management challenges. Our NGS assays are designed for simplicity and flexibility allowing laboratories greater cross-training on their platform of choice.

 

Platform-specific barcoding allows for sequencing on the Illumina MiSeq, MiniSeq, iSeq 100, and Ion Torrent Ion GeneStudio S5 sequencing systems.


Features and benefits

Next Generation Sequencing (NGS) is a powerful technology offering high resolution typing with greater sample throughput. Nonetheless, many labs find it difficult to adopt due to the increased complexity, dedicated labor requirements and data management challenges.

Designed for simplicity and efficiency, our AllType FASTplex NGS Assay makes NGS accessible and achievable for any laboratory. The assay’s advanced chemistry and comprehensive coverage is complemented by a streamlined workflow for minimal hands-on time and supported by intuitive software for easy sample auditing and enhanced data integration.

Next-Generation Sequencing with the AllType FASTplex NGS Assay


NGS is a powerful but complex technology genotyping. An intelligently simple approach, the One Lambda AllType FASTplex NGS Assay is the only easy, single-test solution supported by streamlined and integrated software.

Why choose the AllType FASTplex NGS solution?

  • One multiplex PCR reaction per sample
  • Early sample pooling
  • Total hands-on time <90 minutes
  • Automated data transfer and analysis

AllType FASTplex – quality with less complexity

The AllType FASTplex chemistry has been enhanced to improve performance, robustness, and reliability. With the newly added Class II exon 1 primer mix, the AllType FASTplex reagents reduce ambiguities, provide comprehensive coverage across 11 loci, and generate highly concordant results at 99.8% or higher, all in a single PCR reaction.

 

Figure 1. AllType FASTplex exon coverage.

Workflow simplicity and reduced hands-on time

The assay can enhance your lab’s efficiency with reduced labor, pipetting, and risk of contamination. By combining fragmentation and barcoding steps into a single reaction, the workflow allows for samples to be pooled early in the protocol so that library preparation can be completed in a single tube.

The protocol has been designed for simplicity, easy implementation, and greater cross-training in the lab. From start to finish, the workflow can be performed in under 7 hours with less than 90 minutes of hands-on time.

 

Figure 2. AllType FASTplex workflow.


Choose the right NGS assay, protocol, and coverage for your lab

Our AllType and AllType FASTplex NGS assays apply a single multiplex reaction to amplify all major Class I and Class II loci, completely removing the need for amplicon pooling. Both provide scalable solutions for low- and high-volume laboratories.

 

  AllType FASTplex NGS AllType NGS
Method Multiplex long-range amplification Multiplex long-range amplification
Turnaround time 1-2 days
  • Barcode and pool samples within the first hour
  • One-tube workflow for ~70% of protocol*
  • Total hands-on time less than 90 minutes
2-3 days
Complexity Medium
(does not require specialized training of lab personnel)
High
Resolution Ultra-high Ultra-high
Throughput High High
Regulatory status RUO
Refer to products for details
RUO and IVD
Refer to products for details
Instruments Illumina MiSeq, MiniSeq, iSeq 100, Ion GeneStudio S5, and Ion GeneStudio S5 plus Illumina MiSeq, MiniSeq, iSeq 100, Ion GeneStudio S5, and Ion GeneStudio S5 plus

* Considers one technologist preparing 24 samples.


Software

Streamlined next generation sequencing solution that's flexible

When the workflow is complete, our TypeStream Visual NGS Analysis Software will begin the automatic analysis of your data while you’re away from your desk. The streamlined interface enables easy sample auditing, bi-directional communication with One Lambda HistoTrac Software as well as data migration into our One Lambda HLA Fusion Software for advanced analytics, such as antibody tracking, crossmatching, and epitope analysis.

Platform-specific barcoding allows for sequencing on the Illumina MiSeq, MiniSeq, iSeq 100, Ion Chef, and Ion GeneStudio S5 sequencing systems.

 

Intuitive data integration

TypeStream Visual software now includes bi-directional communication with HistoTrac, enabling the seamless transfer of results between both systems for better data management, reduced transcription errors, and optimized reporting.

Your results can also be combined in our HLA Fusion Software for advanced analytics, such as antibody tracking, crossmatching, and epitope analysis.

Discover a simple, fast, and integrated solution for high-resolution genotyping!



 

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