High-throughput peptide mapping of trastuzumab using a tandem LC-MS workflow

Key benefits

• Operational simplicity of tandem LC-MS methods setup using Thermo Scientific™ Chromeleon™ CDS with compliance-ready enterprise acquisition of LC-MS data for cGMP environments.
• High-throughput peptide mapping of biopharmaceuticals with at least 25% reduction in analysis time when compared to standard LC operation.
• Tandem LC-MS workflow eliminates MS standby time.
• Excellent column to column retention time reproducibility and PTMs for analysis performed on two separate columns in tandem mode.

Goal

• Demonstrate easy setup of Thermo Scientific™ Vanquish™ Duo UHPLC system for Tandem LC-MS workflow and seamless creation of the instrument methods using Chromeleon CDS.
• Demonstrate time-savings, without loss of instrument performance for peptide mapping analysis of monoclonal antibodies.
• Tandem LC-MS workflow to eliminate MS instrument idle time.

在线阅读