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mRNA的纯度：反相和IEC。工艺过程中mRNA纯度的检测，纯化前或纯化后都可以用
Simultaneous reversed-phase ano Ianion-exchange method scouting with a dual syste em for mRNA impurity determination
Application benefits
- Leverage two different separation chemistries in the same instrument at the same time: ion-exchange(IEX)andion-pairing reversed-phase(IP-RP)
- Scout up to 10 solvents for each column type to find most suitable eluent condition
- Accelerate method development
- Maximize sample knowledge
- Perform mRNA profiling and detection of post-in vitrotranscription(IVT) purification impurities
- Use corrosive mobile phases,such as NaCl 1M,with thefully biocompatible flow path of the Thermo ScientificVanquishDuo UHPLC system
Goal
Determine the most suitable conditions for the detection of post-transcriptiona impurities in mRNA with a time-effective scouting approach
为什么选择该应用方法？
mRNA纯度表征方法：如反相、离子交换等，同时需要两套仪器进行检测。
该应用有什么特点？
DNA pac RP：极具代表性的核酸专用柱，独特的键合方式; DNA Pac 200 RS(Check AN):阴离子交换-核酸专用柱，专有的包裹技术，和官能团键合方法；Vanquish Duo 具有双针进样器、双泵，可以实现一套仪器同时进行两种工作流程。
应用的优势有哪些？
DANpac RP：从小到10-20 nt 寡核苷酸，达到几千nt的核酸都适用；DNA Pac 200 RS(Check AN): 无孔基质对于核酸的碱基对个数没有要求，适用范围更宽。提升分析效率，节省了实验室空间，减少仪器成本。
用了该方法可以获得哪些收益？
专用的反向和离子交换模式，双维度监控mRNA纯化，其对于工艺优化筛选和质量监控尤为适用。
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