使用定制和定制 Plus TaqMan™ 拷贝数测定可以获得高特异性、可重现和易于解读的拷贝数结果。这些测定适合验证研究和大规模样本筛选,可提供一种灵活的靶向方法进行拷贝数分析。

每种定制测定均为正向引物、反向引物和 FAM™ 染料标记的 TaqMan™ MGB 探针的混合物。这些测定简单易用,可在通用寡核苷酸浓度和热循环条件下运行。只需添加 TaqMan™ 基因分型预混液和样本即可生成灵敏且可重现的数据。

TaqMan 拷贝数测定产品比较

有两种定制测定选项可满足您的科研需求(定制 TaqMan® 拷贝数测定和定制 Plus TaqMan® 拷贝数测定)。对于这两种,您都可以向我们的 TaqMan® 测定设计流程提交靶序列,然后该流程便会使用您输入的序列设计定制引物和探针序列。这两个产品之间的主要区别见表1:

特点 定制 Plus TaqMan® 拷贝数测定 定制 TaqMan® 拷贝数测定 预设计的 TaqMan® 拷贝数测定
采用已优化性能的拷贝数特定算法进行设计
仅适用于人类和小鼠测定 
包含 TaqMan® FAM™ 染料标记的 MGB™ 探针和 2 种未标记的 PCR 引物
靶标经过 SNP 和重复测序屏蔽 
基因组特异性检查 
参考测定产品兼容性检查✔(可选)✔(可选) 
提供测定序列  
提供测定背景序列和基因组位置 

轻松订购

浏览 GeneAssist™ 拷贝数测定工作流程生成器。导航到感兴趣的人类或小鼠基因组区域,选择预设计的测定,或者选择靶标区域进行定制 Plus 测定设计。或者,提交预屏蔽序列进行标准定制测定设计或提交引物/探针对序列进行测定综合设计。

订购定制 TaqMan 拷贝数测定

订购信息

货号产品名称10 µL 反应次数(384 孔板)20 µL 反应次数(96 孔板)
4442487定制 Plus TaqMan® 拷贝数测定,SM720360
4442520定制 Plus TaqMan® 拷贝数测定,MED1,500750
4442488定制 Plus TaqMan® 拷贝数测定,LG5,8002,900
4400294定制 TaqMan® 拷贝数测定,SM720360
4400295定制 TaqMan® 拷贝数测定,MED1,500750
4400296定制 TaqMan® 拷贝数测定,LG5,8002,900

参考文献

  1. Andrew J Sharp et al.2008.A recurrent 15q13.3 microdeletion syndrome associated with mental retardation and seizures.Nature Genetics Vol 40 No 3 322-28
  2. H. Mefford et al.2008.Recurrent Rearrangements of Chromosome 1q21.1 and Variable Pediatric
  3. Phenotypes.N Engl J Med 359.N Engl J Med 10.1056/nejmoa0805384
  4. Holly N. Cukier et al.2009.Sample degradation leads to false-positive copy number variation calls in multiplex real-time polymerase chain reaction assays.Anal Biochem 386(2):288-90
  5. Leanne M. Dibbens et al.2009.Familial and sporadic 15q13.3 microdeletions in idiopathic generalized epilepsy: precedent for disorders with complex inheritance.Human Molecular Genetics Vol. 18, No. 19 3626–31 doi:10.1093/hmg/ddp311
  6. Spencer KL et al.2010.Dissection of Chromosome 16p12 Linkage Peak Suggests a Possible Role for CACNG3 Variants in Age-Related Macular Degeneration Susceptibility.Invest Ophthalmol Vis Sci [Epub ahead of print]
  7. Güney Bademci et al.2010.A Rare Novel Deletion of the Tyrosine Hydroxylase Gene in Parkinson Disease.Hum Mutat.E1767-71 Online
  8. Louise C. Laurent et al.2011.Dynamic Changes in the Copy Number of Pluripotency and Cell Proliferation Genes in Human ESCs and iPSCs during Reprogramming and Time in Culture.Cell Stem Cell 8, 106–118
  9. Ping Mayo et al.2010.CNV Analysis Using TaqMan® Copy Number Assays.Current Protocols in Human Genetics 2.13.1-2.13.10
  10. Anuradha Ramamoorthya et al.2010.Differential quantification of CYP2D6 gene copy number by four different quantitative real-time PCR assays.Pharmacogenetics and Genomics Vol 20 No 7 452-54
  11. R. Stephanie Huang et al.2009.Population-specific GSTM1 copy number variation.Human Molecular Genetics Vol. 18, No. 2 366–72
  12. Amanda K. Huber et al.Analysis of Immune Regulatory Genes' Copy Number Variants in Graves' Disease.Thyroid 21(1): 69-74. doi:10.1089/thy.2010.0262
  13. Tram Kim Lam et al.2009.Copy Number Variants of GSTM1 and GSTT1 in Relation to Lung Cancer Risk in a Prospective Cohort Study.Ann Epidemiol 19(8):546-52.Epub 2009 Apr 25
  14. Emeli Lundström et al.2011.Effects of GSTM1 in Rheumatoid Arthritis; Results from the Swedish EIRA study.PLoS One 6(3): e17880. doi:10.1371/journal.pone.0017880
  15. Donald F. Conrad et al.2010.Origins and functional effect of copy number variation in the human genome.Nature 464(7289):704-12.Epub 2009 Oct 7
  16. de Kovel CG et al.2010.Recurrent microdeletions at 15q11.2 and 16p13.11 predispose to idiopathic generalized epilepsies.Brain 133(Pt 1):23-32.Epub 2009 Oct 20
  17. Tannour-Louet M et al.2010.Identification of de novo copy number variants associated with human disorders of sexual development.PLoS One 5(10):e15392
  18. Tuch BB et al.Tumor transcriptome sequencing reveals allelic expression imbalances associated with copy number alterations.PLoS One 5(2):e9317
  19. Hakan Thonberg et al.2011.Mutation screening of patients with Alzheimer disease identifies APP locus duplication in a Swedish patient.BMC Research Notes 4:476
  20. Nori Matsunami et al.2013.Identification of Rare Recurrent Copy Number Variants in High-Risk Autism Families and Their Prevalence in a Large ASD Population.PLoS One 8(1):e52239
  21. Low copy number of the salivary amylase gene predisposes to obesity
    VOLUME 46.NUMBER 5.MAY 2014 Nature Genetics
    Mario Falchi et. al.