LanthaScreen Kinase Assay Basic Training Module

• Kinases
• LanthaScreen TR-FRET Technology
• LanthaScreen Kinase Activity Assay
• LanthaScreen Eu Kinase Binding Assay

• Plate readers
• Plates
• Molecular weight information and conversions
• The 1X, 2X, 3X, and 4X notations
• Compound serial dilutions

LanthaScreen Kinase Activity Assays

• Reagents required
• Quick reference protocol
• Step 1: Determine the EC₈₀ of kinase at the apparent ATP Km
• Step 2: Determine the IC₅₀ of a control compound

LanthaScreen Eu Kinase Binding Assays

• Reagents required
• Quick reference protocol
• Determine the IC50 of a control compound
• Detailed Assay Procedure

Calculation worksheets for LanthaScreen Kinase Activity Assay

• Kinase for EC80 determination
• 4X (EC80) Kinase for IC50 determinations
• 2X Substrate/2X ATP

Calculation worksheets for LanthaScreen Eu Kinase Binding Assay

• 3X Tracer
• 3X Kinase/3X antibody