Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes:

Cells were transduced with CellLight® Lysosomes-GFP (Cat. No. C10596) and CellLight® Mitochondria-RFP (Cat. No. C10601). Following treatment with chloroquine to induce autophagosome accumulation, nuclei were stained with Hoechst 33342 (Cat. No. H24192) and live-cell imaging was performed using standard DAPI/FITC/TRITC filter sets.

Cells were transduced with CellLight® Lysosomes-GFP (Cat. No. C10596) and CellLight® Mitochondria-RFP (Cat. No. C10601). Following treatment with chloroquine to induce autophagosome accumulation, nuclei were stained with Hoechst 33342 (Cat. No. H24192) and live-cell imaging was performed using standard DAPI/FITC/TRITC filter sets.

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