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Microtubules of fixed bovine pulmonary artery endothelial cells (BPAEC) were localized with mouse monoclonal anti–a-tubulin antibody (Cat. no. A11126), followed by the biotin-XX–conjugated F(ab)2 fragment of goat anti–mouse IgG antibody (Cat. no. B11027) and visualized with green-fluorescent Alexa Fluor® 488 streptavidin (Cat. no. S11223). The cells were counterstained with blue-fluorescent Hoechst 33342 (Cat. no. H1399, H3570, H21492) to image the DNA and red-fluorescent propidium iodide (Cat. no. P1304MP, P3566, P21493) to image nucleolar RNA. The multiple-exposure image was acquired using bandpass filter sets appropriate for Texas Red® dye, fluorescein and DAPI.
A prometaphase muntjac skin fibroblast stained with Alexa Fluor® 350 phalloidin, an anti–a-tubulin antibody and an anti–cdc6 peptide antibody. Go ›
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›
1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›
Developing Drosophila embryo Go ›
Endothelial cells. Go ›
Muntjac fibroblast labeled with probes for actin and the nucleus. Go ›
Simultaneous detection of expression of five genes in a whole mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes Go ›
Multicolor fluorescence analysis of muntjac fibroblasts. Go ›
Nuclear, cytoskeletal, and mitochondrial staining of Muntjac fibroblasts. Go ›
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody Go ›