Neutrophil migrating through a gelatin matrix. DQ Green BSA and dihydrotetramethylrosamine.

Unlabeled neutrophils that have been allowed to migrate spontaneously in gelatin matrices containing a prototype of DQ Green BSA (D12050) and dihydrotetramethylrosamine. Extracellular release of reactive oxygen metabolites oxidizes dihydrotetramethylrosamine to tetramethylrosamine, yielding orange fluorescence. Extracellular protease activity cleaves highly quenched DQ Green BSA, yielding green-fluorescent peptides. The micrograph illustrates an alternating pattern of orange and green fluorescence and shows that extracellular release of oxidants and proteolytic activity oscillate 180° out of phase during cell locomotion. Image contributed by Howard R. Petty, Wayne State University.

Unlabeled neutrophils that have been allowed to migrate spontaneously in gelatin matrices containing a prototype of DQ Green BSA (D12050) and dihydrotetramethylrosamine. Extracellular release of reactive oxygen metabolites oxidizes dihydrotetramethylrosamine to tetramethylrosamine, yielding orange fluorescence. Extracellular protease activity cleaves highly quenched DQ Green BSA, yielding green-fluorescent peptides. The micrograph illustrates an alternating pattern of orange and green fluorescence and shows that extracellular release of oxidants and proteolytic activity oscillate 180° out of phase during cell locomotion. Image contributed by Howard R. Petty, Wayne State University.

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