Endogenous alkaline phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head.

Endogenous phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head was localized using the ELF 97 Endogenous Phosphatase Detection Kit (E6601) to stain a cryosection. In addition to the yellow-green fluorescence of the ELF 97 alcohol precipitate, the section was stained with red-fluorescent Texas Red-X wheat germ agglutinin (W21405) and with the blue-fluorescent Hoechst 33342 nucleic acid stain (H1399, H3570, H21492). The triple-exposure image was acquired using bandpass filter sets appropriate for ELF 97 alcohol, Texas Red dye and DAPI.

Endogenous phosphatase activity of osteoblast cells in a cartilaginous element of an adult zebrafish head was localized using the ELF 97 Endogenous Phosphatase Detection Kit (E6601) to stain a cryosection. In addition to the yellow-green fluorescence of the ELF 97 alcohol precipitate, the section was stained with red-fluorescent Texas Red-X wheat germ agglutinin (W21405) and with the blue-fluorescent Hoechst 33342 nucleic acid stain (H1399, H3570, H21492). The triple-exposure image was acquired using bandpass filter sets appropriate for ELF 97 alcohol, Texas Red dye and DAPI.

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