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Fixed and permeabilized bovine pulmonary artery endothelial cells (BPAEC) labeled with anti–OxPhos Complex IV subunit I (human) antibody (anti–cytochrome oxidase subunit I) (Cat. no. A6403) and detected using TSA Kit #6 with the HRP conjugate of goat anti–mouse IgG antibody and Alexa Fluor® 647 tyramide (Cat. no. T20916). The image was deconvolved using Huygens software (Scientific Volume Imaging, http://www.svi.nl/).