Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide OBEA-dCTP nucleotides.

Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide OBEA-dCTP nucleotides. Probes specific to the alpha satellite sequences from human chromosome 17, chromosome 15 and chromosome 1 were labeled using nick translation with ChromaTide Alexa Fluor 488-7-OBEA-dCTP (green, C21555), ChromaTide Alexa Fluor 594-7-OBEA-dCTP (red) and ChromaTide Alexa Fluor 647-12-OBEA-dCTP (pink, C21559), respectively. The probes were hybridized to metaphase spreads from peripheral blood lymphocyte cultures. The chromosomes were counterstained with Hoechst 33342 (blue) (H1399, H3570, H21492).

Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide OBEA-dCTP nucleotides. Probes specific to the alpha satellite sequences from human chromosome 17, chromosome 15 and chromosome 1 were labeled using nick translation with ChromaTide Alexa Fluor 488-7-OBEA-dCTP (green, C21555), ChromaTide Alexa Fluor 594-7-OBEA-dCTP (red) and ChromaTide Alexa Fluor 647-12-OBEA-dCTP (pink, C21559), respectively. The probes were hybridized to metaphase spreads from peripheral blood lymphocyte cultures. The chromosomes were counterstained with Hoechst 33342 (blue) (H1399, H3570, H21492).

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