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Sample Preparation for Protein Mass |
Protein sample preparation remains one of the largest bottlenecks associated with mass spectrometry (MS) analysis. Consistent and reproducible protein sample preparation for liquid chromatography mass spectrometry (LC/MS) can make the difference between a successful analysis and a failed outcome.
Thermo Scientific reagents and kits are optimized for protein to peptide sample preparation for every step of the process, from depletion strategies to reduce complexity, to protein extraction, enrichment, digestion, and clean-up for the isolation of low-abundance proteins. Post digestion, peptide enrichment, fractionation, and clean-up steps are often critical for proper analysis. The colorimetric and fluorometric Quantitative Peptide Assays enable sample normalization following digestion
Protein Sample Preparation Kits for LC/MS Analysis
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EasyPep MS Sample Preparation KitsProtein extraction from samples through peptide clean-up EasyPep MS Sample Preparation kits are designed for mass spectrometry analysis. Kits come in different formats to suit a variety of sample amounts, sample quantities, and in column, plate, or magnetic bead processing. |
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AccelerOme Automated Sample Preparation KitsSample preparation kits designed to work in the Thermo Scientific AccelerOme automated sample preparation platform. Kits include all components needed to transfer from a manual proteomic sample preparation to hands-free processing. | |
Protein Extraction
Cell lysis is the first step when extracting proteins for mass spectrometry applications. Fractionation may be required to enrich for proteins of interest or to study protein localization.
Abundant Protein Depletion
Kits for albumin, top 2, or top 12 abundant protein depletion to aid in removing the most abundant proteins so that detection of lower abundant proteins is enhanced prior to digestion.
Protein Enrichment and Clean Up
Enrichment techniques include immunoprecipitation, enrichment by PTM (such as phosphoprotein enrichment), or active site labeling and enrichment using activity-based probes.
Protein Interaction Crosslinking
Crosslinking in combination with mass spectrometry is a powerful method to improve protein characterization and identification of protein-protein interactions.
Protein Digestion
Proteases to break the proteins of interest into smaller fragments, or peptides for mass spectrometry analysis.
Peptide Enrichment and Fractionation
Successful analysis of low-abundant proteins and identification of post-translationally modified peptides requires multiple steps: enrichment, fractionation and/or clean up.
C18 Columns and Peptide Desalting
After isolation of peptides, salts and buffers can be removed using reversed phase (RP) resins, of which the C18 matrix is the most ideal for the capture of hydrophobic peptides.
Peptide Quantitation Assays
The colorimetric and fluorescent peptide quantitation assays help to normalize the peptide concentration of samples during each processing step following protein digestion.
Detergent Removal from Peptides
Thermo Scientific Pierce Detergent Removal Resins are provided in convenient spin-column or plate formats that quickly and efficiently remove ionic, nonionic, and/or zwitterionic detergents from protein or peptide samples to improve compatibility with downstream applications.
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For Research Use Only. Not for use in diagnostic procedures.