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Cloning Methods
When you are trying out a new cloning method or simply looking for a recommendation this section covers common cloning methods and techniques.
Blunt End CloningZero Blunt TOPO Cloning Kits eliminates background making cloning blunt-ended products efficient and fast. |
Clone CollectionsEliminate RNA isolation, cDNA synthesis, and sequence validation using open reading frame clones |
Long Fragment CloningTOPO cloning, Zero Background technology and an unique gel purification step to enhance cloning of PCR products from 3-10 kb. |
Restriction Enzyme Digestion and LigationCloning by restriction enzyme digestion and ligation is a simple and easy way of moving a fragment of double stranded DNA from one plasmid to another. |
Plant DNA CloningCloning plant DNA is a key step for genetic engineering, gene studies, and other applications in plant research. |
MutagenesisGeneArt Site-Directed Mutagenesis System provides a convenient and highly efficient means to generate mutant target proteins in vitro. |
Gene SynthesisGene synthesis enables you to design, construct and optimize your gene(s) of interest. |
Seamless Cloning & Gibson Assembly®GeneArt Gibson Assembly and Seamless Cloning allow you to build clones using multiple fragments with no additional sequences or scars. |
TOPO PCR Cloning5-minute, room-temperature cloning with no gel purification or post-PCR modifications and up to 95% recombinants with TOPO cloning. |
RecombinationSimplify cloning and expression of multiple genes using Gateway technology. |
TransformationTransformation is the process of altering a cell so that it acquires and expresses genetic material acquired from the surrounding environment. |
Thermo Scientific Cloning Tools
FastDigest restriction enzymes, CloneJET kits, Ligase Independent Cloning, Alkaline Phosphatases and more.
Resources
Tools
Cloning Services
We offer everything from custom vector design to high-throughput protein expression.
Gibson Assembly® is a registered trademark of SGI-DNA, Inc. used under permission and license.
For Research Use Only. Not for use in diagnostic procedures.