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Molecular Cloning Essentials |
Innovative products for the molecular cloning workflow
The molecular cloning workflow requires multiple steps to obtain high-quality cloned DNA for your downstream applications. Help advance your molecular cloning workflow at every step and accelerate construction of recombinant clones.
Our wide range of cloning solutions address needs from routine to specialty applications, helping you find optimal reagents to achieve fast results with greater confidence.
Molecular cloning workflow
Click on each step to explore the molecular cloning workflow and select products for your downstream applications that fit your research.
Select from a variety of reagents and instruments to generate your fragment of interest from DNA, RNA, or synthetic fragments.
PCR Enzymes & Reagents
PCR enzymes for genotyping and routine PCR. Characterized by high fidelity and high specificity, these enzymes are suitable for amplifying long fragments and GC-rich templates.
Reverse Transcription
Reverse transcriptase synthesizes cDNA from single-stranded RNA and serves as a template for PCR amplification. Explore our selection tool to find the right reverse transcriptase for your experiment.
Oligonucleotides
Custom DNA Oligos Synthetic Services provide oligos in a variety of scales, formats, modifications, and purifications. Use our easy ordering portal for oligos that meet your cloning needs.
Thermal Cyclers
Applied Biosystems thermal cyclers are designed for reliability and accuracy that helps generate consistent and reproducible PCR results. Use with validated PCR and qPCR plastics for optimal fit and performance.
PCR Plastics
Explore the online plastics selection tool to find the right fit and compatible plastics for your PCR or qPCR instruments. Validated for use with Applied Biosystems thermal cyclers.
Nucleotides
dNTPs, NTPs, and modified nucleotides are synthesized from high-quality raw materials in state-of-the-art production facilities and are available in a variety of formats, formulations, and volumes for convenience and flexibility.
Strings DNA Fragments
GeneArt Strings DNA Fragments are custom-made, uncloned, double-stranded linear DNA fragments assembled from synthetic oligonucleotides. Excellent for multi-fragment assembly with Gibson Assembly cloning kits.
PureLink DNA Purification Kits
PureLink DNA purification kits are an easy, fast, and safe method for isolating DNA fragments that yield excellent results. Provides purified DNA fragments ready for your cloning applications.
GeneJET DNA Purification Solutions
GeneJET nucleic acid purification kits rely on convenient spin column-based technology that offers reliable and affordable purification of DNA and RNA from different sources to meet your cloning needs.
Up next: Step 2, Cloning ›
Discover reagents and techniques across the molecular cloning workflow, with solutions ranging from restriction enzymes to gene synthesis, designed for speed and simplicity to help you obtain high-quality cloned DNA for your next discovery.
FastDigest Restriction Enzymes
FastDigest restriction enzymes are an advanced line of 176 restriction enzymes in a complete, one-buffer system for simplified cloning. Performs complete digestion in 5–15 minutes and is compatible with downstream applications.
Modifying Enzymes
Modifying enzymes offer reliable, high-quality, high-purity reagents, including DNA and RNA polymerases, phosphatases, kinases, and nucleases for an efficient cloning workflow.
DNA Vectors
A wide selection of vectors available in kits and as a stand-alone product to transfer foreign genetic material into a host cell for your downstream applications.
TOPO PCR Cloning
TOPO PCR kits enable cloning in 5 minutes with three easy steps, including Blunt-End, TA and Long Fragment cloning kits. Explore our selection tool to find the right kits for your cloning experiments.
Gateway Cloning Technology
Gateway recombination cloning can hellp empower gene shuttling, enabling you to access virtually any expression system, including E. coli, mammalian, and insect, in just a few simple steps.
Gibson Assembly® Cloning
Gibson Assembly® cloning kits are designed for efficient and seamless assembly of multiple DNA fragments of varying length into any vector. Excellent for multi-fragment assembly with GeneArt Strings DNA Fragments.
GeneArt Seamless Cloning
GeneArt Seamless Cloning kits enable the simultaneous and directional assembly of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector for diverse applications.
GeneArt Gene Synthesis Services
GeneArt Gene Synthesis is a reliable and cost-effective method for obtaining custom DNA fragments and constructs with 100% sequence accuracy. Optimize expression of synthetic genes, with our patented GeneOptimizer algorithm.
Thermo Scientific Cloning Tools
Thermo Scientific branded cloning tools, including PCR cloning kits, bacterial expression kits, cloning enzymes, competent cells, and DNA vectors for efficient cloning.
Discover more about the protein expression workflow
Up next: Step 3, Transformation
The choice of competent cells for transformation depends upon the transformation methods, strain genotypes, plasmid characteristics, and desired applications. Explore a wide range of chemically competent and electrocompetent E. coli cells for low- and high-throughput cloning.
Competent Cells Portfolio
Choose exceptional competent cells for your cloning applications and workflow from our wide range of single-use and high throughput, chemically competent and electrocompetent E. coli strains.
Chemically Competent Cells
Chemically competent cells are the most frequently used format in a wide array of applications and are transformed by heat shock.
Electrocompetent Cells
Electrocompetent cells are often preferred for library creation and low DNA input cloning applications due to very high transformation efficiencies achieved by electroporation.
Competent Cell Formats
Competent cells are available in versatile packaging formats, from single-use to high throughput, to fit your unique cloning workflow requirements.
Bacterial Growth Media
Choose from a wide selection of high quality ready-to-use or dehydrated E. coli bacterial growth media formats to suit your microbial culture needs.
Thermo Scientific Competent Cells
Thermo Scientific branded competent cells including, DH10B, DH5α, and BL21(DE3) for reliable and consistent performance for your cloning workflows.
Up next: Step 4, Plasmid preparation ›
Once the bacterial cells are transformed with the vector and constructs selected, it is critical to recover plasmid DNA with maximum yield, purity, and integrity. Choose a method best suited to your workflow from column-based, magnetic bead-based, or automated solutions.
Plasmid DNA Extraction
Plasmid DNA extraction kits for plasmid DNA isolation at the purity and scale you need. Choose from our wide range of high-performing, cost-effective plasmid purification technologies.
PureLink Purification Solutions
PureLink plasmid DNA kits are available in a variety of formats, from molecular biology grade to transfection-grade, to fit your downstream experiments.
GeneJET Plasmid Miniprep Kit
GeneJET plasmid DNA extraction kit for high purity, high yield plasmid DNA isolation. Achieved by using short protocols and suitable for use with common downstream applications.
MagJET Plasmid DNA Kit
MagJET plasmid DNA extraction kits for fast and efficient purification of plasmid DNA. Magnetic bead-based technology utilizes high-capacity paramagnetic particles optimized to isolate nucleic acids with excellent purity and yields.
Kingfisher Duo Prime Purification System
The Kingfisher Duo Prime system automates the purification or isolation of DNA, RNA, proteins, and cells from virtually any source to deliver exceptional flexibility and performance.
Up next: Step 5, Construct analysis ›
Choosing the right tools for your cloning analysis can significantly improve and accelerate results, enabling you to address downstream applications sooner. Select either the Nanodrop or Qubit benchtop instruments for your DNA sample quantification. Discover our innovative E-Gel Power Snap Electrophoresis systems, and explore our precast E-gel options to help accelerate your DNA analysis.
E-Gel Power Snap Electrophoresis Systems
E-Gel Power Snap integrated electrophoresis systems with the ability to run, visualize, and image all on one platform. The three-step workflow is easy and convenient—just load, run, and analyze.
E-Gel Precast Agarose Gels
E-Gel precast agarose gels provide ready-to-use, safe, and convenient DNA electrophoresis analysis without gels to pour, buffers to make, or chemical staining. Available in a variety of well formats, percentages, and throughput for downstream applications.
UltraPure Agarose and Reagents
UltraPure agarose and reagents for your nucleic acid analysis and purification needs. Made from our highest purity biochemicals for maximum reliability and superior performance.
DNA Stains
Versatile gel stains for nucleic acid visualization, sizing, and quantification. Explore the SYBR Safe stain—a safer alternative to ethidium bromide—and our high-sensitivity SYBR Gold nucleic acid stain for exceptional performance.
DNA Ladders
DNA ladders are available in a variety of size ranges, and formats for convenient DNA sizing and quantification. Stable through multiple freeze-thaw cycles and includes loading dyes for easy tracking of your DNA samples.
Thermo Scientific Nucleic Acid Electrophoresis Products
Thermo Scientific brand of electrophoresis products suitable for everyday nucleic acid electrophoresis applications throughout the molecular biology workflow.
NanoDrop Microvolume Spectrophotometer
NanoDrop is a benchtop instrument for convenient sample quantification and purity analysis of DNA, RNA, and proteins. NanoDrop family products allow for the analysis of microvolumes of concentrated samples.
Qubit 4 Fluorometer
The Qubit 4 benchtop fluorometer empowers fast and accurate fluorescence-based DNA, RNA, and protein quantification for difficult-to-process samples.
Select from a variety of reagents and instruments to generate your fragment of interest from DNA, RNA, or synthetic fragments.
PCR Enzymes & Reagents
PCR enzymes for genotyping and routine PCR. Characterized by high fidelity and high specificity, these enzymes are suitable for amplifying long fragments and GC-rich templates.
Reverse Transcription
Reverse transcriptase synthesizes cDNA from single-stranded RNA and serves as a template for PCR amplification. Explore our selection tool to find the right reverse transcriptase for your experiment.
Oligonucleotides
Custom DNA Oligos Synthetic Services provide oligos in a variety of scales, formats, modifications, and purifications. Use our easy ordering portal for oligos that meet your cloning needs.
Thermal Cyclers
Applied Biosystems thermal cyclers are designed for reliability and accuracy that helps generate consistent and reproducible PCR results. Use with validated PCR and qPCR plastics for optimal fit and performance.
PCR Plastics
Explore the online plastics selection tool to find the right fit and compatible plastics for your PCR or qPCR instruments. Validated for use with Applied Biosystems thermal cyclers.
Nucleotides
dNTPs, NTPs, and modified nucleotides are synthesized from high-quality raw materials in state-of-the-art production facilities and are available in a variety of formats, formulations, and volumes for convenience and flexibility.
Strings DNA Fragments
GeneArt Strings DNA Fragments are custom-made, uncloned, double-stranded linear DNA fragments assembled from synthetic oligonucleotides. Excellent for multi-fragment assembly with Gibson Assembly cloning kits.
PureLink DNA Purification Kits
PureLink DNA purification kits are an easy, fast, and safe method for isolating DNA fragments that yield excellent results. Provides purified DNA fragments ready for your cloning applications.
GeneJET DNA Purification Solutions
GeneJET nucleic acid purification kits rely on convenient spin column-based technology that offers reliable and affordable purification of DNA and RNA from different sources to meet your cloning needs.
Up next: Step 2, Cloning ›
Discover reagents and techniques across the molecular cloning workflow, with solutions ranging from restriction enzymes to gene synthesis, designed for speed and simplicity to help you obtain high-quality cloned DNA for your next discovery.
FastDigest Restriction Enzymes
FastDigest restriction enzymes are an advanced line of 176 restriction enzymes in a complete, one-buffer system for simplified cloning. Performs complete digestion in 5–15 minutes and is compatible with downstream applications.
Modifying Enzymes
Modifying enzymes offer reliable, high-quality, high-purity reagents, including DNA and RNA polymerases, phosphatases, kinases, and nucleases for an efficient cloning workflow.
DNA Vectors
A wide selection of vectors available in kits and as a stand-alone product to transfer foreign genetic material into a host cell for your downstream applications.
TOPO PCR Cloning
TOPO PCR kits enable cloning in 5 minutes with three easy steps, including Blunt-End, TA and Long Fragment cloning kits. Explore our selection tool to find the right kits for your cloning experiments.
Gateway Cloning Technology
Gateway recombination cloning can hellp empower gene shuttling, enabling you to access virtually any expression system, including E. coli, mammalian, and insect, in just a few simple steps.
Gibson Assembly® Cloning
Gibson Assembly® cloning kits are designed for efficient and seamless assembly of multiple DNA fragments of varying length into any vector. Excellent for multi-fragment assembly with GeneArt Strings DNA Fragments.
GeneArt Seamless Cloning
GeneArt Seamless Cloning kits enable the simultaneous and directional assembly of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector for diverse applications.
GeneArt Gene Synthesis Services
GeneArt Gene Synthesis is a reliable and cost-effective method for obtaining custom DNA fragments and constructs with 100% sequence accuracy. Optimize expression of synthetic genes, with our patented GeneOptimizer algorithm.
Thermo Scientific Cloning Tools
Thermo Scientific branded cloning tools, including PCR cloning kits, bacterial expression kits, cloning enzymes, competent cells, and DNA vectors for efficient cloning.
Discover more about the protein expression workflow
Up next: Step 3, Transformation
The choice of competent cells for transformation depends upon the transformation methods, strain genotypes, plasmid characteristics, and desired applications. Explore a wide range of chemically competent and electrocompetent E. coli cells for low- and high-throughput cloning.
Competent Cells Portfolio
Choose exceptional competent cells for your cloning applications and workflow from our wide range of single-use and high throughput, chemically competent and electrocompetent E. coli strains.
Chemically Competent Cells
Chemically competent cells are the most frequently used format in a wide array of applications and are transformed by heat shock.
Electrocompetent Cells
Electrocompetent cells are often preferred for library creation and low DNA input cloning applications due to very high transformation efficiencies achieved by electroporation.
Competent Cell Formats
Competent cells are available in versatile packaging formats, from single-use to high throughput, to fit your unique cloning workflow requirements.
Bacterial Growth Media
Choose from a wide selection of high quality ready-to-use or dehydrated E. coli bacterial growth media formats to suit your microbial culture needs.
Thermo Scientific Competent Cells
Thermo Scientific branded competent cells including, DH10B, DH5α, and BL21(DE3) for reliable and consistent performance for your cloning workflows.
Up next: Step 4, Plasmid preparation ›
Once the bacterial cells are transformed with the vector and constructs selected, it is critical to recover plasmid DNA with maximum yield, purity, and integrity. Choose a method best suited to your workflow from column-based, magnetic bead-based, or automated solutions.
Plasmid DNA Extraction
Plasmid DNA extraction kits for plasmid DNA isolation at the purity and scale you need. Choose from our wide range of high-performing, cost-effective plasmid purification technologies.
PureLink Purification Solutions
PureLink plasmid DNA kits are available in a variety of formats, from molecular biology grade to transfection-grade, to fit your downstream experiments.
GeneJET Plasmid Miniprep Kit
GeneJET plasmid DNA extraction kit for high purity, high yield plasmid DNA isolation. Achieved by using short protocols and suitable for use with common downstream applications.
MagJET Plasmid DNA Kit
MagJET plasmid DNA extraction kits for fast and efficient purification of plasmid DNA. Magnetic bead-based technology utilizes high-capacity paramagnetic particles optimized to isolate nucleic acids with excellent purity and yields.
Kingfisher Duo Prime Purification System
The Kingfisher Duo Prime system automates the purification or isolation of DNA, RNA, proteins, and cells from virtually any source to deliver exceptional flexibility and performance.
Up next: Step 5, Construct analysis ›
Choosing the right tools for your cloning analysis can significantly improve and accelerate results, enabling you to address downstream applications sooner. Select either the Nanodrop or Qubit benchtop instruments for your DNA sample quantification. Discover our innovative E-Gel Power Snap Electrophoresis systems, and explore our precast E-gel options to help accelerate your DNA analysis.
E-Gel Power Snap Electrophoresis Systems
E-Gel Power Snap integrated electrophoresis systems with the ability to run, visualize, and image all on one platform. The three-step workflow is easy and convenient—just load, run, and analyze.
E-Gel Precast Agarose Gels
E-Gel precast agarose gels provide ready-to-use, safe, and convenient DNA electrophoresis analysis without gels to pour, buffers to make, or chemical staining. Available in a variety of well formats, percentages, and throughput for downstream applications.
UltraPure Agarose and Reagents
UltraPure agarose and reagents for your nucleic acid analysis and purification needs. Made from our highest purity biochemicals for maximum reliability and superior performance.
DNA Stains
Versatile gel stains for nucleic acid visualization, sizing, and quantification. Explore the SYBR Safe stain—a safer alternative to ethidium bromide—and our high-sensitivity SYBR Gold nucleic acid stain for exceptional performance.
DNA Ladders
DNA ladders are available in a variety of size ranges, and formats for convenient DNA sizing and quantification. Stable through multiple freeze-thaw cycles and includes loading dyes for easy tracking of your DNA samples.
Thermo Scientific Nucleic Acid Electrophoresis Products
Thermo Scientific brand of electrophoresis products suitable for everyday nucleic acid electrophoresis applications throughout the molecular biology workflow.
NanoDrop Microvolume Spectrophotometer
NanoDrop is a benchtop instrument for convenient sample quantification and purity analysis of DNA, RNA, and proteins. NanoDrop family products allow for the analysis of microvolumes of concentrated samples.
Qubit 4 Fluorometer
The Qubit 4 benchtop fluorometer empowers fast and accurate fluorescence-based DNA, RNA, and protein quantification for difficult-to-process samples.
Featured resources for molecular cloning
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