Cypridina Luciferase Assays & Vectors

We offer luciferase reporter reagents, multiple cloning site vectors, and flash, glow and dual-spectral luciferase assay kits based on the naturally secreted Cypridina luciferase. The Cypridina luciferase is a 61kDa protein from the marine ostracod, Cypridina noctiluca. Cypridina luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output captured using a luminometer can be correlated with the amount of Cypridina luciferase protein produced and used to determine the activity of the promoter driving Cypridina expression.

The Cypridina gene in Thermo Scientific Pierce Cypridina Luc Vectors is modified for expression in mammalian cells and provides optimal performance with the assay kit. The Cypridina glow reagent yields a Cypridina luciferase reaction with glow-type kinetics and is recommended for luminometers without injectors or for applications requiring batch processing of samples. 

Cypridina luciferin, also known as Vargulin, substrate is oxidized by Cypridina luciferase, producing Cypridina oxyluciferin, carbon dioxide and light.