NovaFluor Ultra Violet 765 excitation shown as dotted line and emission shown as solid red histogram
3349-358763(in buffer) 5
flow cytometry

Invitrogen NovaFluor Ultra Violet 765 dye (NovaFluor UV 765) has a unique emission spectrum when excited by the 349 nm laser, which fits in a spectral space that is currently not accessible by other spectral dyes. Adding spectrally unique dyes may allow for building out larger panels with minimal loss of resolution.

NovaFluor UV 765 dye should generally be paired with moderately to highly expressed antigens in multicolor panels. The macromolecule-based NovaFluor UV 765 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

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Benefits of NovaFluor UV 765 dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Should be paired with moderately expressed antigens to minimize spillover
  • Spectrally unique and can be used to fill unused detectors in conventional flow cytometry
  • Produces stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor UV 765 dye

Spectral signature of NovaFluor UV 765 dye. Normal human peripheral blood cells stained with anti-CD4 antibody (clone SK3) conjugated to NovaFluor UV 765 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system. 

3-panel scatter plot of human PBMCs with CD3, CD4, CD8, CD45RA, and CD27 staining.
 Click image to enlarge

NovaFluor UV 765 dye is compatible with spectrally adjacent fluorophores, BUV615, BUV661, BUV737, and BUV805, in the same experiment. Human peripheral blood cells (PBMCs) were stained with CD3 Monoclonal Antibody, Brilliant Ultra Violet™ 737 (left), CD4 NovaFluor UV 765 dye, CD8a Monoclonal Antibody, Brilliant Ultra Violet™ 805 (middle), CD45RA Monoclonal Antibody, Brilliant Ultra Violet™ 661, and CD27 Monoclonal Antibody, Brilliant Ultra Violet™ 615 (right). The CD3-positive gate was used for analysis in the middle and right plots. Viable cells were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation. Data was collected on a 5-laser Cytek Aurora Flow Cytometer.

NovaFluor dyes

NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

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Cy™ is a trademark or registered trademark of GE Healthcare.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.