NovaFluor Blue 530 excitation shown as dotted line and emission shown as solid green histogram
1488525/18508527(in buffer) 5
flow cytometry

Invitrogen NovaFluor Blue 530 dye is a bright, green-fluorescent dye with excitation designed for use with the 488 nm laser line. With a similar excitation spectrum and narrower emission spectrum, it is a good alternative to other dyes with similar emission profiles such as FITC and Alexa Fluor 488 dye.

NovaFluor Blue 530 dye should generally be paired with moderately to highly expressed antigens in multicolor panels. The macromolecule-based NovaFluor Blue 725 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Search NovaFluor Blue 530 primary antibodies

Benefits of NovaFluor Blue 530 dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Can serve as a replacement for FITC or Alexa Fluor 488 dye with a cleaner emission spectra
  • Can be used in a flow cytometry panel with NovaFluor Blue 510 and NovaFluor Blue 555 dyes
  • Produces highly stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor Blue 530 dye

Spectral signature of NovaFluor Blue 530 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Blue 530 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
 

2-panel scatter plots of mouse splenocytes, unstained vs CD3e-stained and CD45R-stained vs CD3e-stained

Staining of mouse splenocytes with NovaFluor Blue 530 conjugated antibody. BALB/c mouse splenocytes were unstained (left) or stained with 1 µg of CD45R Monoclonal Antibody, NovaFluor Blue 530 (right). All cells were co-stained with CD3e Monoclonal Antibody, eFluor 450. Total viable cells were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation. Data was acquired on a 5-laser Cytek Aurora system and unmixed with autofluorescence extraction.

2-panel scatter plots of human PBMCs, unstained vs. CD19-stained and HLA-DR-stained vs. CD19-stained
Staining of human peripheral blood cells with NovaFluor Blue 530 conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with HLA-DR Monoclonal Antibody, NovaFluor Blue 530 (right). All cells were co-stained with CD19 Monoclonal Antibody, eFluor 450. Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation. Data was acquired on a 5-laser Cytek Aurora system.

NovaFluor dyes

NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

Additional resources

Cy™ is a trademark or registered trademark of GE Healthcare.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.

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