Pacific Green dye

Pacific Green dye is a bright green-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 411/510 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.

Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm and 551 nm, respectively, facilitating three-color analysis.

We offer Pacific Green dye conjugated to a variety of primary antibodies, secondary antibodies, and streptavidin. In addition, reactive dyes and protein labeling kits are available so that you can produce your own antibody conjugates or probes.

Pacific Green dye dashboard


Initial brightness
 
 
 
 
 
Pacific Green dye is a green fluorophore used primarily for flow cytometry, optimally excited by the 405 nm violet laser line. 
  

Photostability in buffer
 
 
 
 
 
 17405FITC410500 
 
Stain index
Laser line
Common filter set
Excitation max
Emission max		 

Pacific Green dye products

We offer a range of Pacific Green dye products including labeling kits, labeled antibodies, bioconjugates, and more.

Pacific Green™ dye

Fluorescence excitation and emission spectra of Pacific Green dye.

Pacific Green dye reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

Amine

Violet laser–compatible antibody conjugates

Life Technologies offers Pacific Blue, Pacific Green and Pacific Orange dyes as well as Qdot probes for use with the violet laser. In addition to primary and secondary antibody conjugates of these dyes, we’ve developed kits for cell proliferation, immunophenotyping, dead cell discrimination, apoptosis, and cell cycle analysis—all aimed at helping you maximize the data you can get from your violet laser–equipped flow cytometer.

probesonline-may-2013.par.96977.image.320.285.1.pol-may-2013-pacific-green-jpg
Excitation spectra (dotted lines) and emission spectra (solid lines) for Molecular Probes violet laser–excited Pacific Blue dye (blue), Pacific Green dye (green), and Pacific Orange dye (orange). The shaded area represents the emission bandpass filter (522/31) used for detection of the Pacific Green dye.