List of Tables

Chapter 1

• Molecular Probes amine-reactive dyes—Table 1.1
• Active esters and kits for labeling proteins and nucleic acids—Table 1.2
• Molecular Probes kits for protein and nucleic acid labeling—Table1.3
• Alexa Fluor active esters and kits for labeling proteins and nucleic acids—Table 1.4
• Fluorescence quantum yields (QY) and lifetimes (τ) for Alexa Fluor dyes—Table 1.5
• R₀ values for some Alexa Fluor dyes—Table 1.6
• Amine-reactive BODIPY dyes—Table 1.7
• Amine-reactive xanthene derivatives—Table 1.8
• Amine-reactive, orange- and red-fluorescent fluorophores—Table 1.9
• Molecular Probes nonfluorescent quenchers and photosensitizers—Table 1.10
• R₀ values for QSY and dabcyl quenchers—Table 1.11
• Amine-reactive, ultraviolet light–excitable fluorophores for labeling proteins and nucleic acids—Table 1.12
• Amine-reactive, environment-sensitive fluorophores—Table 1.13

Chapter 2

• Molecular Probes thiol-reactive dyes excited with visible light—Table 2.1
• Molecular Probes thiol-reactive dyes excited with ultraviolet light—Table 2.2

Chapter 3

• Molecular Probes azide and alkyne derivatives—Table 3.1
• Molecular Probes hydrazine, hydroxylamine and amine derivatives—Table 3.2

Chapter 4

• Biotinylation and desthiobiotinylation reagents—Table 4.1
• Selected haptenylation reagents and their anti-hapten antibodies—Table 4.2
• Sensitivity of the FluoReporter Biotin Quantitation Assay Kit for biotinylated nucleic acids with varying degrees of biotinylation—Table 4.3

Chapter 5

• Molecular Probes heterobifunctional crosslinkers—Table 5.1
• Properties of six different caging groups—Table 5.2

Chapter 6

• Tyramide Signal Amplification (TSA) Kits—Table 6.1
• Spectral data for B-PE, R-PE and APC—Table 6.2
• Tandem conjugates of R-phycoerythrin (R-PE)—Table 6.3
• Tandem conjugates of allophycocyanin (APC)—Table 6.4
• Molecular Probes yellow-green–fluorescent FluoSpheres beads compared with other commercially available yellow-green–fluorescent microspheres—Table 6.5
• Fluorescein equivalents in Molecular Probes yellow-green–fluorescent FluoSpheres beads—Table 6.6
• Summary of FluoSpheres fluorescent microspheres—Table 6.7
• Summary of biotin-, streptavidin- and NeutrAvidin biotin-binding protein–labeled FluoSpheres microspheres—Table 6.8
• Summary of TransFluoSpheres fluorescent microspheres—Table 6.9
• Extinction coefficients of Qdot nanocrystal streptavidin conjugates at ultraviolet and visible wavelengths—Table 6.10
• Qdot nanocrystal secondary antibody conjugates—Table 6.11
• Qdot nanocrystal anti–human CD antibody conjugates—Table 6.12

Chapter 7

• Summary of Molecular Probes secondary antibody conjugates—Table 7.1
• Molecular Probes goat anti-mouse isotype-specific antibodies—Table 7.2
• Alkaline phosphatase and horseradish peroxidase enzyme conjugates—Table 7.3
• Fluorescent dyes successfully tested with the Image-iT FX signal enhancer—Table 7.4
• NANOGOLD, Alexa Fluor FluoroNanogold and colloidal gold conjugates—Table 7.5
• Binding profiles of protein A and protein G—Table 7.6
• Zenon Antibody Labeling Kits—Table 7.7
• Anti-fluorophore and anti-hapten antibodies—Table 7.8
• Molecular Probes avidin, streptavidin, NeutrAvidin and CaptAvidin conjugates—Table 7.9
• Molecular Probes lectin conjugates—Table 7.10

Chapter 8

• Specialty nucleic acid reagents for molecular biology—Table 8.1
• Cell membrane–impermeant cyanine nucleic acid stains—Table 8.2
• Cell-permeant cyanine nucleic acid stains—Table 8.3
• Properties of classic nucleic acid stains—Table 8.4
• Characteristics of Molecular Probes ChromaTide and aha labeled nucleotides—Table 8.5
• Molecular Probes nucleic acid labeling kits—Table 8.6
• Amine-reactive dyes for nucleic acid sequencing—Table 8.7
• Selection guide for the Qubit and Quant-iT Assay Kits—Table 8.8

Chapter 9

• A comparison of reagents for detecting and quantitating proteins in solution—Table 9.1
• SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9.2
• Molecular Probes fluorescent and luminescent protein blot stains—Table 9.3
• Pro-Q Diamond gel stain reagents and kits—Table 9.4
• Pro-Q Emerald glycoprotein stain kits for gels and for blots—Table 9.5

Chapter 10

• Glycosidase enzymes and their fluorogenic and chromogenic substrates—Table 10.1
• Rhodamine 110–based bis-peptide substrates—Table 10.2
• RediPlate Assay Kits—Table 10.3
• Detection limits of the EnzChek Protease Assay Kits—Table 10.4
• Advantages of the Amplex UltraRed reagent over chromogenic reagents—Table 10.5

Chapter 11

• CellLight reagents and their targeting sequences—Table 11.1
• Spectral characteristics of Molecular Probes actin-selective probes—Table 11.2

Chapter 12

• Molecular Probes fluorescent organelle stains—Table 12.1
• Spectral characteristics of the MitoTracker probes—Table 12.2
• Summary of the LysoTracker and LysoSensor probes—Table 12.3

Chapter 13

• Phospholipids with labeled head groups—Table 13.1
• Spectral properties of some lipid probes—Table 13.2

Chapter 14

• Techniques for loading molecules into the cytoplasm—Table 14.1
• Fluorescence characteristics of NeuroTrace fluorescent Nissl stains—Table 14.2
• Summary of Molecular Probes lipophilic carbocyanine and aminostyryl tracers—Table 14.3
• Molecular Probes dextran conjugates—Table 14.4
• FluoSpheres microspheres for blood flow determination—Table 14.5
• FluoSpheres blood flow and color kits—Table 14.6
• FluoSpheres and TransFluoSpheres microspheres for tracing—Table 14.7
• Molecular Probes europium and platinum luminescent FluoSpheres microspheres—Table 14.8

Chapter 15

• Esterase substrates for cell viability studies—Table 15.1
• Molecular Probes assay kits for cell viability, cell counting and bacterial gram staining—Table 15.2
• Some organisms that have been successfully stained with our LIVE/DEAD BacLight Bacterial Viability Kits—Table 15.3
• Molecular Probes apoptosis assay kits—Table 15.4
• Fluorogenic substrates for caspase activity—Table 15.5
• Fluorescent detection reagents for imaging mitochondria and lysosomes—Table 15.6

Chapter 16

• Fluorescent lipopolysaccharide conjugates—Table 16.1
• Transferrin conjugates—Table 16.2
• BioParticles fluorescent bacteria and yeast—Table 16.3
• Labeled and unlabeled alpha-bungarotoxins—Table 16.4

Chapter 17

• Invitrogen kinase assay platforms—Table 17.1
• Spectroscopic properties of MANT-ATP in aqueous solution (pH 8)—Table 17.2
• Fluorescence-based phospholipase assays—Table 17.3

Chapter 18

• Reactive oxygen species—Table 18.1
• Scavengers of reactive oxygen species (ROS)—Table 18.2
• Tetrazolium salts for detecting redox potential in living cells and tissues—Table 18.3
• Fluorescence response of APF, HPF and H2DCFDA to various reactive oxygen species (ROS)—Table 18.4

Chapter 19

• Summary of Molecular Probes fluorescent Ca2+ indicators—Table 19.1
• Comparison of in vitro and in situ Kd values for various Ca2+ indicators—Table 19.2
• Parallel performance comparison of fluo-3 and fluo-4 on Molecular Devices FLIPR system—Table 19.3
• Coelenterazines and their properties—Table 19.4
• Response of fura-2 and indo-1 to some divalent cations other than Ca²⁺ and Mg²⁺—Table 19.5
• Fluorescent indicators for Zn²⁺—Table 19.6
• Ca2+ affinities of BAPTA chelators—Table 19.7

Chapter 20

• Molecular Probes pH indicator families, in order of decreasing pKa—Table 20.1
• Summary of the pH response of LysoSensor probes—Table 20.2
• Molecular Probes pH indicator dextrans, in order of decreasing pKa—Table 20.3
• Reactive pH indicator dyes—Table 20.4

Chapter 22

• Characteristics and selected applications of Molecular Probes fast-response probes—Table 22.1
• Characteristics and selected applications of Molecular Probes slow-response probes—Table 22.2

Chapter 23

• Spectral characteristics of Molecular Probes dyes—Table 23.1
• Fluorescence excitation sources—Table 23.2
• Contents of FocalCheck fluorescence microscope tests slides—Table 23.3
• Spectroscopic data for components of the Reference Dye Sampler Kit—Table 23.4
• CultureWell cell culture systems and chambered coverslips—Table 23.5
• CoverWell chamber gaskets—Table 23.6
• Press-to-Seal gaskets and Secure-Seal spacers—Table 23.7
• Tools for hybridization experiments—Table 23.8
• Spectral characteristics of Cell Sorting Set-Up Beads—Table 23.9